This is the first time I'm posting in this forum, so I apologize if I'm reposting something. I've read through most of these threads but I may of missed something. This topic has certainly been touched on before, but I wanted to get more to the point in this thread.
My first minor point: based on my read of the paper I see no reason that fasting could have been a confounding factor in the life extension part of the experiment documented in the paper. The line regarding fasting the rats overnight only appears in the section titled "Pharmokinetics" in which the researchers document sacrificing those same rats 48 hours later for organ collection. As far as I can tell, this was a totally different experiment. These are not the same rats actually used in the life extension study. The methods we're interested in are in the section entitled "2.3 Chronic toxicity and effects of C60 on survival of rats."
Now, for my next, more important point, a quote from the original paper:
2.3. Chronic toxicity and effects of C60 on survival of rats
The rats were housed three per cage and acclimated for 14 days, before dosing. Three groups of 6 rats (10months old, weighing 465±31 g) were administered daily for one week, then weekly until the end of the second month and then every two weeks until the end of the 7th month, by gavages with 1 ml of water or olive oil or C60 dissolved in olive oil (0.8 mg/ml), respectively.
I read this as the dosing was relatively infrequent most of the time, and also short relative to the lives of the rats. By the end of month 17, no rats were getting any more C60. Nevertheless, we still observed this significant lifespan increase, which is especially promising since C60 is said by the authors to be cleared from the GI tract in 10s of hours.
"Pharmacokinetic studies show that dissolved C60 is absorbed by the gastrointestinal tract and eliminated in a few tens of hours." (from the abstract)
Based on what people have been experimenting with in animals and themselves it seemed like this point about dosing was not well understood, so I just wanted to make it clear to everyone.
Reason for optimism?