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Cellular differentiation process degenerates while aging?

aging theory degeneration cells

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#1 Jose_LER

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Posted 20 August 2014 - 12:34 PM


Anyone has information about this subject?

I mean, if you've read something about aging and a worse cellular differentiation.



#2 tunt01

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Posted 22 August 2014 - 03:36 AM

I had a conference call with a stem scientist in the last year and the person remarked that the regenerative capacity (or proliferative capacity) of stem cells can vary by age of the individual/source tissue.  We were having a discussion about stem cell "quality" and the lack of benchmarking (rate of differentiation/cellular division, ability to heal damaged tissue, etc.).  I think your comment is in keeping with this scientist's remarks, but I do not have a reference for you and I cannot quote the individual directly as the discussion was confidential.



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#3 Jose_LER

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Posted 22 August 2014 - 12:52 PM

I had a conference call with a stem scientist in the last year and the person remarked that the regenerative capacity (or proliferative capacity) of stem cells can vary by age of the individual/source tissue.  We were having a discussion about stem cell "quality" and the lack of benchmarking (rate of differentiation/cellular division, ability to heal damaged tissue, etc.).  I think your comment is in keeping with this scientist's remarks, but I do not have a reference for you and I cannot quote the individual directly as the discussion was confidential.

 

Thanks for the answer.

I was reading some information for years about this subject and I found a possible explanation for the phenomena.

The differentiation mechanism is related with some electrical characteristics of the living system and it could be engineered in some way.

 

I've a theory, only just I've to develop a study to prove if the theory is true or not. I think it's true.



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#4 niner

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Posted 22 August 2014 - 03:12 PM

Stem cells maintain their "stemness" by getting their energy from glycolysis instead of oxphos.  When they differentiate, they need to switch on the oxphos process.  We've seen a few suggestions that stem cell differentiation is aided or encouraged in some way by c60oo, which also appears to enhance mitochondrial function.  I hypothesize that in some cases, the switch from glycolysis to oxphos does not proceed smoothly, and the c60 is providing a bit of a nudge that helps it get going. 



#5 Jose_LER

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Posted 22 August 2014 - 10:10 PM

Stem cells maintain their "stemness" by getting their energy from glycolysis instead of oxphos.  When they differentiate, they need to switch on the oxphos process.  We've seen a few suggestions that stem cell differentiation is aided or encouraged in some way by c60oo, which also appears to enhance mitochondrial function.  I hypothesize that in some cases, the switch from glycolysis to oxphos does not proceed smoothly, and the c60 is providing a bit of a nudge that helps it get going. 

 

So, reading your post is possible that a cell differentation process would work with less efficiency if some energy released from some oxidative process is less efficient.

 

I mean, if a oxidative process is performed with less efficiency then the energy from that oxidative process that is needed to differentiate the cell would be less efficient.



#6 niner

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Posted 22 August 2014 - 10:27 PM

 

Stem cells maintain their "stemness" by getting their energy from glycolysis instead of oxphos.  When they differentiate, they need to switch on the oxphos process.  We've seen a few suggestions that stem cell differentiation is aided or encouraged in some way by c60oo, which also appears to enhance mitochondrial function.  I hypothesize that in some cases, the switch from glycolysis to oxphos does not proceed smoothly, and the c60 is providing a bit of a nudge that helps it get going. 

 

So, reading your post is possible that a cell differentation process would work with less efficiency if some energy released from some oxidative process is less efficient.

 

I mean, if a oxidative process is performed with less efficiency then the energy from that oxidative process that is needed to differentiate the cell would be less efficient.

 

Well, not just any oxidative process, but oxidative phosphorylation specifically.  In other words, mitochondrial respiration.  Also bear in mind that this is just a hypothesis.  We don't as of yet have any solid evidence for it.



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#7 Jose_LER

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Posted 22 August 2014 - 10:47 PM

 

 

Stem cells maintain their "stemness" by getting their energy from glycolysis instead of oxphos.  When they differentiate, they need to switch on the oxphos process.  We've seen a few suggestions that stem cell differentiation is aided or encouraged in some way by c60oo, which also appears to enhance mitochondrial function.  I hypothesize that in some cases, the switch from glycolysis to oxphos does not proceed smoothly, and the c60 is providing a bit of a nudge that helps it get going. 

 

So, reading your post is possible that a cell differentation process would work with less efficiency if some energy released from some oxidative process is less efficient.

 

I mean, if a oxidative process is performed with less efficiency then the energy from that oxidative process that is needed to differentiate the cell would be less efficient.

 

Well, not just any oxidative process, but oxidative phosphorylation specifically.  In other words, mitochondrial respiration.  Also bear in mind that this is just a hypothesis.  We don't as of yet have any solid evidence for it.

 

 

I see. The things I'm writting here they're an hypothesis too. I could be true too...

Imagine that the oxidative processes emits small electrical impulses that activates the cellular differentiation. An incomplete or imperfect chemical process makes less precise electrical impulses, affecting them to the differentiation.

 



#8 Jose_LER

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Posted 09 October 2014 - 02:54 PM

Stem cells maintain their "stemness" by getting their energy from glycolysis instead of oxphos.  When they differentiate, they need to switch on the oxphos process.  We've seen a few suggestions that stem cell differentiation is aided or encouraged in some way by c60oo, which also appears to enhance mitochondrial function.  I hypothesize that in some cases, the switch from glycolysis to oxphos does not proceed smoothly, and the c60 is providing a bit of a nudge that helps it get going. 

 

You say that Stem Cells maintain their "stemness" by getting their energy from glycolisys.

When they differentiate they need to switch on the oxphos process.

 

Very interesting....

 

Some time ago I thought about an experimental way about selectively cellular differentiation based on a very similar idea.

Even, it could be adapted to a computer software to mimic biological processes and differientiate that cell into a specific one using a device and the software.



#9 Danail Bulgaria

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Posted 09 October 2014 - 04:14 PM

Anyone has information about this subject?

I mean, if you've read something about aging and a worse cellular differentiation.

 

Unfortyunately, the cells, after diffeentiate, stop multiplying. 
 



#10 Jose_LER

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Posted 09 October 2014 - 04:22 PM

 

Anyone has information about this subject?

I mean, if you've read something about aging and a worse cellular differentiation.

 

Unfortyunately, the cells, after diffeentiate, stop multiplying. 
 

 

 

Well, maybe we've to see 2 clear processes. I will call to the first "specialized differentiation" and to the second "replacement differentiation".

 

Specialized differentiation would be the process where a pluripotential cell (a cell that can be specialized into any other different) becomes differentiated into a different cell.

 

Replacement differentiation would be the process where a cell dies and a new cell, exactly the same as the last one, is created.

 

In real cases this process could be mixed since the body tries to create a new cell to replace the old one that has just died, but the body has not enought resources to create exactly the same. Maybe the body creates a cell that is 99.99% the same as the old one, but there is a difference of the 0.01% that is accumulative and while we age that accumulation takes place more and more and we compare a cell from 30 years ago and an actual cell and we see evidential changes.

Why these changes? Because that 0.01% has been acumulating for 30 years and now the difference could be 15% if we compare a cell from 30 years ago with an actual cell.

 

All the percentages are imaginative, I don't know the real percentage.
 



#11 Jose_LER

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Posted 09 October 2014 - 04:47 PM

There is something almost evident, and it's the following...

 

When regeneration processes take place, there are 2 things that are common: the first is that there is a change in the electrical properties of the cells and the second is that there is an activation of different genes. So the common thing is that celullar polarization could activate some specific genes. When the regeneration has finished the electrical properties change and they are in equilibrium. That means that those genes are not active at the end because regeneration has took place and finished. So there would be a relation between electricity and gene activation.

 

Of course, the next question is... how electricity activates/deactivates the genes?

Electricity can vary mainly in polarization and intensity. There are thousands or even more genes.

So the activation/deactivation process is a very specific electrical mechanism. If you combine the maximum combinations of polarization (positive or negative) and intensity, there is not enought combinations to selectively activate the genes because you see that the different genes are much more bigger (in number) than the polarity/intensity combinations. So there would be another different electrical characteristic involved in that process.

 

I think it would be the spectrum? Humans communicate using voice spectrums and signatures. Could be cells use a similar mechanism to communicate between them? If so, it could answer the question about having enought combinations to selectively activate the genes. There are thousands of signatures, while there are very few polarization/intensity combinations.

 

I think intensity is almost constant while polarization, depending the anabolic or catabolic process, there are clearly 2: positive and negative.



#12 Danail Bulgaria

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Posted 09 October 2014 - 04:50 PM

I don't know. Science is getting more and more radical. I will not be surprised, if this also happens to be true.



#13 Jose_LER

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Posted 09 October 2014 - 05:11 PM

I don't know. Science is getting more and more radical. I will not be surprised, if this also happens to be true.

 

If I don't remember bad.. there is some information that says that sunlight radiation activates some gene(s) that produces skin cancer.



#14 Danail Bulgaria

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Posted 09 October 2014 - 05:14 PM

 

I don't know. Science is getting more and more radical. I will not be surprised, if this also happens to be true.

 

If I don't remember bad.. there is some information that says that sunlight radiation activates some gene(s) that produces skin cancer.

 

 

The ultra-violet light actually damages the genome. It is supposed, that this is the way for it to trigger cancer.
 



#15 Jose_LER

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Posted 09 October 2014 - 05:18 PM

 

 

I don't know. Science is getting more and more radical. I will not be surprised, if this also happens to be true.

 

If I don't remember bad.. there is some information that says that sunlight radiation activates some gene(s) that produces skin cancer.

 

 

The ultra-violet light actually damages the genome. It is supposed, that this is the way for it to trigger cancer.
 

 

 

If biophoton are related with the UV spectrum, then it's possibly to modulate the bio-oxidative processes from external sources. That gives as a result a possibility to regulate some biochemical and bioenergetic processes... Interesting.

 

But I think that is only a small part of the whole. There should be more, a living system is more complex than that.
 



#16 Danail Bulgaria

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Posted 09 October 2014 - 05:37 PM

 

If biophoton are related with the UV spectrum, then it's possibly to modulate the bio-oxidative processes from external sources.

 

The UV spectrum influence the oxidative processes - it creates free radicals.



#17 corb

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Posted 09 October 2014 - 08:54 PM

Cellular differentiation process degenerates while aging?

Ok I'm not super versed in the subject but I'll tell you in short what I know.

Now, we know for sure a number of types of damage happen with age :

>mutations to your DNA

#certain regions in your DNA controll the work of cellular organelle's like the ribosomes

#the ribosomes fabricate the proteins needed for cellular communication

#so damage to the rDNA will result in impeded cellular communication

#you need that communication for cellular differentiation

 

>mutation to mDNA

#mitochondia can grow and mutate and impede the workings of cells themselves

 

>the extracellular matrix gets filled with junk molecules which probaly impedes communication to some degree as well

 

And so on. Any damage that happens to the cell (and probably the extra cellular matrix) impedes differentiation.

 

You'd be interested in the research that goes on into stem cells because a lot of that is centered around making the stem cells differentiate.


Edited by corb, 09 October 2014 - 08:57 PM.


#18 Logic

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Posted 10 October 2014 - 10:00 AM

This is quite a can of worms you are opening here and you are likely to be figuratively tarred and feathered on this forum unless you have done extensive research and have all your ducks I a row.

The hugely popular thread 'Lostfalco's Extensive Nootropic Experiments' is largely about using IR light at the right frequencies to do good things for mental function.
Then you have the Rife machines that just wont die and a couple of other 'out there' inventions.
http://www.rexresearch.com/

I have also seen some papers on PubMed about potential (voltage differences) etc.
Good luck

#19 corb

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Posted 10 October 2014 - 12:43 PM

This is quite a can of worms you are opening here and you are likely to be figuratively tarred and feathered on this forum unless you have done extensive research and have all your ducks I a row.

 

Electric shock can be and is used on cell cultures in labs for different purposes. It's really not that much OUT THERE. It's the effects that people expect to get out of it that are out there, not the process in itself.

 

 Electroporation, or electropermeabilization, is a significant increase in the electrical conductivity and permeability of the cell plasma membrane caused by an externally applied electrical field. It is usually used in molecular biology as a way of introducing some substance into a cell, such as loading it with a molecular probe, a drug that can change the cell's function, or a piece of coding DNA.[1]

 

There are lots of papers on pubmed about how cells react to different electrical simulations, when it's something as simple as this you have to bet on that someone tried it before, it's really not that fringe when it can be done in half an hour with cheap materials in any university lab.



#20 corb

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Posted 10 October 2014 - 04:00 PM

I realized I was mostly answering Jose and it's too late to edit the post so a little addendum, am about light affecting cells, I know they use lasers in genetic engineering, so unless for some reason they are using a method that can risk the outcome of millions of dollars of r&d on purpose, I'd think scientist are sure at this point that light cannot directly affect the cell in anyway besides the already observed ones.

Basically it's another topic that's been researched.



#21 mpe

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Posted 10 October 2014 - 11:31 PM

I'm only a layman, my biology education is my high school education and the internet, having said that.

There are many theories of aging and most just don't work and will result in wasting millions to billions of dollars and many valuable years in failed research in attempting to slow, stop and reverse aging.

So far I have only read one theory which appears to explain aging in a comprehensive way, and it will upset the majority of this forums members as it was not proposed by Aubrey de Grey.

Its the theory of Programmed Aging and its explained very well by Harold Katcher Phd in the attached pdf.

 

 

Mike

 

 

 

 

 

Attached Files



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#22 scottknl

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Posted 11 October 2014 - 01:43 AM

I've attended a lecture on muscle stem cell differentiation and aging and some great work has been done in this line by Irina Conboy from UC Berkley.

 

 

 







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