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How to measure intracellular inflammation?

inflammation

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#1 InquilineKea

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Posted 09 October 2014 - 11:15 PM


C-reactive protein is usually low-sensitivity, and doesn't say everything about what's going on (especially in the cell).



#2 Darryl

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Posted 10 October 2014 - 06:01 PM

In animal studies, commonly levels of activated transcription factor NF-kB (a master regulator of inflammatory response) are measured using binding affinity of cell protein extracts to NF-κB binding site DNA (electromobility/electrophoretic mobility shift assay). Expression of a wider range of inflammation-related mRNAs can be done with real-time quantitative PCR. Usually the animals are sacrificed for tissue samples.

 

Quantitative expression analysis for inflammation related genes could be done with a clinical gene expression analysis kit using blood samples (kits like this are being tested for clinical diagnosis in other diseases), but I haven't come across a commercially available test like this for a broad range of inflammation markers. As gene expression can vary a lot more during the day and after meals than markers like CRP, I'm not sure an inflammatory gene expression test would have the clinical utility to justify the cost. 

 







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