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Peptide profiling of Internet-obtained Cerebrolysin using high performance liquid chromatography

peptide cerebrolysin hplc

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#1 Metagene

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Posted 31 May 2015 - 12:47 PM


I can't wait to take a peek at this baby!

Cerebrolysin, a parenteral peptide preparation produced by controlled digestion of porcine brain proteins, is an approved nootropic medicine in some countries. However, it is also easily and globally available on the Internet. Nevertheless, until now, its exact chemical composition was unknown. Using high performance liquid chromatography (HPLC) coupled to ion trap and ultra high performance liquid chromatography (UHPLC) coupled to quadrupole-ion mobility-time-of-flight mass spectrometry (Q-IM-TOF MS), combined with UniProt pig protein database search and PEAKS de novo sequencing, we identified 638 unique peptides in an Internet-obtained Cerebrolysin sample. The main components in this sample originate from tubulin alpha- and beta-chain, actin, and myelin basic protein. No fragments of known neurotrophic factors like glial cell-derived neurotrophic factor (GDNF), neurotrophin nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and ciliary neurotrophic factor (CNTF) were found, suggesting that the activities reported in the literature are likely the result of new, hitherto unknown cryptic peptides with nootropic properties. Copyright © 2015 John Wiley & Sons, Ltd.


http://www.ncbi.nlm....ubmed/26017115/

Edited by Metagene, 31 May 2015 - 12:48 PM.

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#2 Fenix_

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Posted 31 May 2015 - 08:42 PM

Can you get the full article? I wonder if cerebrolysin has any effect on myelination and/or glial cells.


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#3 Metagene

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Posted 01 June 2015 - 04:30 PM

Can you get the full article? I wonder if cerebrolysin has any effect on myelination and/or glial cells.


No not thru Libgen.

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#4 Metagene

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Posted 11 August 2015 - 01:03 AM

Gatcha!

https://drive.google...dF9PYUFPOWFlNkE
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