14 replies to this topic
#1
Mondey
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Posted 19 May 2012 - 11:42 AM
Selfmade microglia
To start with the microglia replacement therapy we need lots of cells and they must resemble the original microglia. In the pictures you see microglia isolated from mice (left) or self-made microglia derived from bone marrow (in vitro differentiated).
In both cases they have basically the same morphology. In case of the self-made microglia we only have more...
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#2
Mondey
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Posted 18 May 2012 - 08:04 PM
Microglia Stem Cells: Community update
In 2011, the Longecity community raised money to support a research project on microglia stem cell therapy.  Over the next few days, we will be reporting interim results in this blog.
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#3
Mondey
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Posted 20 May 2012 - 10:37 PM
Brain histology - microglia
To visualize microglia and amyloid plaques in vivo, we established different staining protocols (including histology and immunohistology) to later evaluate microglia number after transplantation and also amyloid load. Top left is showing an immuno staining for amyloid and top right is an overview for microglia in the...
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#4
Mondey
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Posted 19 May 2012 - 12:02 PM
Charaterisation of microglia
We used several markers to prove that what looks like microglia really are microglia. We used so called surface markers (CDs) including CD11b and CD45. These markers are also used to distinguish between what people all macrophages and what people think microglia are (there are a lot of discussions about the identity of microglia). The cells in the red...
View the full blog post
#5
Mondey
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Posted 25 June 2012 - 09:52 AM
Transplantations
Hi everybody,
more news from the project. After months of delays to get the animal experiment approval we now transplanted our Alzheimer mice with microglia cells. You see here one of your group members preparing the different animal groups (with and without cells). I hope that all will be going well and I will report back once the animals have been...
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#6
Mind
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Posted 28 October 2012 - 01:04 PM
Thanks for keeping
everyone up to date on the research Mondey. Looks like you are getting close to the point where we will have some quantifiable data on amyloid.
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#7
Mondey
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Posted 08 October 2012 - 10:02 PM
Some transplantations done - more to come
Hi again,
so we have started with our transplantations and some of our animals have been analysed. You see a picture showing our transplanted microglia in green and the amyloid in red. You can notice that the microglia are around/ or in the vessels. We do know from our PCR data that we get quite some microglia into the brain but it seems from these pictures...
View the full blog post
#8
Mondey
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Posted 08 October 2012 - 10:14 PM
Amyloid load
As for the effect the transplanted microglia have on the pathology: I do not see a big difference in the amount of amyloid in the Alzheimer mouse brain without cells (A) and with microglia transplanted (B). So we have to wait until we get all brains analysed by sterology for the exact amyloid quantification.
Greetings,
Dey
View the full blog post
#9
Mondey
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Posted 19 May 2012 - 11:42 AM
Selfmade microglia
To start with the microglia replacement therapy we need lots of cells and they must resemble the original microglia. In the pictures you see microglia isolated from mice (left) or self-made microglia derived from bone marrow (in vitro differentiated).
In both cases they have basically the same morphology. In case of the self-made microglia we only have more...
View the full blog post
#10
Mondey
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Posted 18 May 2012 - 08:04 PM
Microglia Stem Cells: Community update
In 2011, the Longecity community raised money to support a research project on microglia stem cell therapy.  Over the next few days, we will be reporting interim results in this blog.
View the full blog post
#11
Mondey
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Posted 08 October 2012 - 10:02 PM
Some transplantations done - more to come
Hi again,
so we have started with our transplantations and some of our animals have been analysed. You see a picture showing our transplanted microglia in green and the amyloid in red. You can notice that the microglia are around/ or in the vessels. We do know from our PCR data that we get quite some microglia into the brain but it seems from these pictures...
View the full blog post
#12
Mondey
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69 posts
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Posted 20 May 2012 - 10:37 PM
Brain histology - microglia
To visualize microglia and amyloid plaques in vivo, we established different staining protocols (including histology and immunohistology) to later evaluate microglia number after transplantation and also amyloid load. Top left is showing an immuno staining for amyloid and top right is an overview for microglia in the...
View the full blog post
#13
Mondey
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Posted 19 May 2012 - 12:02 PM
Charaterisation of microglia
We used several markers to prove that what looks like microglia really are microglia. We used so called surface markers (CDs) including CD11b and CD45. These markers are also used to distinguish between what people all macrophages and what people think microglia are (there are a lot of discussions about the identity of microglia). The cells in the red...
View the full blog post
#14
Mondey
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Posted 18 May 2012 - 08:25 PM
Background
As a reminder WHY we are doing this:
What are microglia:
Microglia comprise about 10% of the cell population of the brain and form the main first immune defense of the CNS. They are phagocytic, cytotoxic, present antigens and they can promote repair after injury (Simard 2007).
Microglia in the brain display normally a quiescent state in which...
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#15
Mondey
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Posted 08 October 2012 - 10:14 PM
Amyloid load
As for the effect the transplanted microglia have on the pathology: I do not see a big difference in the amount of amyloid in the Alzheimer mouse brain without cells (A) and with microglia transplanted (B). So we have to wait until we get all brains analysed by sterology for the exact amyloid quantification.
Greetings,
Dey
View the full blog post
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