Edited by Dream Big, 18 November 2021 - 08:18 AM.
New cryopreservation method is much better
#1
Posted 18 November 2021 - 08:15 AM
#2
Posted 27 November 2021 - 02:33 PM
Here is the full paper. One thing I noticed was the article linked above stated that the cell survival increased a ton, but the paper mentions nothing about any such testing or in what way they survived (cloning? eating fish food (if used a fish?)?). Perhaps the article was hyping it. However the paper does mention the model, and that may be of great importance, even if there was no such real experiment yet.
They are on vacation until mid December, so we may need to wait to ask questions by email.
#3
Posted 30 November 2021 - 12:26 PM
Oh, I guess I forgot to upload the paper (probably due to the 2nd needed click it requires to attach it). Here:
Attached Files
#4
Posted 01 December 2021 - 11:35 PM
Interesting. Thanks for sharing.
I didn't read the whole paper, but it seems to be saying that the model predicts better cryopreservation, but this method has not been tested in real tissues yet. Is that right?
#5
Posted 02 December 2021 - 09:08 AM
I took another look at the article in my opening post, and see now some parts that mention ex. "earlier research", ah. Let me paste below the middle of the article that is all you need to read and I'll highlight some areas too to pay attention to. Then below I'll summarize what this probably means and answer your question:
“The problem is that these chemicals can cause osmotic damage due to water crossing cell membranes and causing the cells to burst,” Higgins said. “They can also kill cells due to toxicity. So in designing the best vitrification method, the trick is choosing the best path between normal physiological conditions and a final vitrified state – i.e., high CPA concentration and liquid nitrogen temperature.”
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