All:
Cross-posting this from the Methuselah Foundation Forums, to stop people from hearing about this elsewhere and running out to take a black-market, potentially fatal drug ...
Calorie restriction is the most effective non-genetic intervention to enhance life span known to date. A major research interest has been the development of therapeutic strategies capable of promoting the beneficial results of this dietary regimen.
In this sense, we propose that compounds that decrease the efficiency of energy conversion, such as mitochondrial uncouplers, can be caloric restriction mimetics.
Treatment of mice with low doses of the protonophore 2,4-dinitrophenol promotes enhanced tissue respiratory rates, improved serological glucose, triglyceride and insulin levels, decrease of reactive oxygen species levels and tissue DNA and protein oxidation, as well as reduced body weight [and occasionally kills people from thermogenic fever -MR].
Importantly, dinitrophenol-treated animals also presented enhanced longevity. [Importantly, this is nonsense -- see below -MR]
Our results demonstrate that mild mitochondrial uncoupling is a highly effective in vivo antioxidant strategy, and describe the first therapeutic intervention capable of effectively reproducing the physiological, metabolic and life span effects of caloric restriction in healthy mammals. (1)
Well, first, lots of other therapeutic interventions have reproduced some of the physiological and metabolic effects of CR; the question is whether a given agent reproduces the important ones (the ones responsible for retarding the aging process). Unfortunately, we still don't know quite what those effects are ...
On the longevity claim: this is just 'the usual nonsense': ALL the animals were short-lived, and those that got DNP lived a little longer, but still not nearly as long as a normal, healthy, well-husbanded, non-genetically-messed-up mouse (which will on av'g live ~900 days) (2). In this study, "Median longevity in the control group was 722 days, versus 771 days in the DNP group, while mean lifespan was 718.8 days in the control and 769.7 days in the treated group. Although the change in life span promoted by DNP was not large, it is statistically significant (p = 0.038). " . And there was no extension of maximum LS at all, even compared to the short-lived controls; extension of max LS is the very essence of the CR life extension effect, which is exactly what marks it as the sole genuine anti-aging intervention validated in mammals to date)
The human analogy would be a study in which you took a bunch of people , put half of them on a dangerous drug for their entire lives, and at the end of the study the average control lived just 67 years (rather than 82, you'd expect from a random sample of the USA) -- but look! People taking the drug lived to be 70! It's a miracle anti-aging drug!!
What happened? I note that "Food intake was also equal in both groups (Fig. 1c), while DNP-treated animals presented significantly lower body mass (Fig. 1d)"; I'm going to GUESS that they just let the mice eat themselves into obesity, and DNP partially corrected for the ensuing metabolic mayhem.
The conclusion is not that DNP is an anti-aging drug; the conclusion is tht DNP can help some sick mice, and these folks need to learn to take better care of them.
-Michael
1. Caldeira da Silva CC, Cerqueira FM, Barbosa LF, Medeiros MH, Kowaltowski AJ.
Mild Mitochondrial Uncoupling in Mice Affects Energy Metabolism, Redox Balance and Longevity.
Aging Cell. 2008 May 23. [Epub ahead of print]
PMID: 18505478
2. Miller RA, Harper JM, Dysko RC, Durkee SJ, Austad SN.
Longer life spans and delayed maturation in wild-derived mice.
Exp Biol Med (Maywood). 2002 Jul;227(7):500-8.
PMID: 12094015