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L-Theanine with Caffeine, underrated nootropic


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#1 Ichoose2live

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Posted 01 January 2011 - 10:02 PM


L-Theanine
L-theanine (N-ethyl-L-glutamine) or theanine is a major amino acid uniquely found in green tea. L-theanine has been historically reported as a relaxing agent, prompting scientific research on its pharmacology. Animal neurochemistry studies suggest that L-theanine increases brain serotonin, dopamine, GABA levels and has micromolar affinities for AMPA, Kainate and NMDA receptors. In addition has been shown to exert neuroprotective effects in animal models possibly through its antagonistic effects on group 1 metabotrophic glutamate receptors. Behavioural studies in animals suggest improvement in learning and memory. Overall, L-theanine displays a neuropharmacology suggestive of a possible neuroprotective and cognitive enhancing agent and warrants further investigation in animals and humans. [1]


L-Theanine Nootropic effects alone.
Background/Objectives Ingestion of the non-proteinic amino acid L-theanine (gamma-glutamylethylamide) has been shown to influence oscillatory brain activity in the alpha band (8-14 Hz) in humans during resting electroencephalographic (EEG) recordings and also during cognitive task performance. We have previously shown that ingestion of a 250-mg dose of L-theanine significantly reduced tonic (background) alpha power during a demanding intersensory (auditory-visual) attentional cueing task. Further, cue-related phasic changes in alpha power, indexing the shorter-term anticipatory biasing of attention between modalities, were stronger on L-theanine compared to placebo. This form of cue-contingent phasic alpha activity is also known to index attentional biasing within visual space. Specifically, when a relevant location is pre-cued, anticipatory alpha power increases contralateral to the location to be ignored. Here we investigate whether the effects of L-theanine on tonic and phasic alpha activity, found previously during intersensory attentional deployment, occur also during a visuospatial task. Subjects/Methods 168-channel EEG data were recorded from thirteen neurologically normal individuals while engaged in a highly demanding visuo-spatial attention task. Participants underwent testing on two separate days, ingesting either a 250-mg colorless and tasteless solution of L-theanine mixed with water, or a water-based solution placebo on each day in counterbalanced order. We compared the alpha-band activity when subjects ingested L-Theanine vs. Placebo. Results We found a significant reduction in tonic alpha for the L-theanine treatment compared to placebo, which was accompanied by a shift in scalp topography, indicative of treatment-related changes in the neural generators of oscillatory alpha activity. However, L-theanine did not measurably affect cue-related anticipatory alpha effects. Conclusions This pattern of results implies that L-theanine plays a more general role in attentional processing, facilitating longer-lasting processes responsible for sustaining attention across the timeframe of a difficult task, rather than affecting specific moment-to-moment phasic deployment processes. [2]

Tea is the most widely consumed beverage in the world after water. Tea is known to be a rich source of flavonoid antioxidants. However tea also contains a unique amino acid, L-theanine that may modulate aspects of brain function in humans. Evidence from human electroencephalograph (EEG) studies show that it has a direct effect on the brain (Juneja et al. Trends in Food Science & Tech 1999;10;199-204). L-theanine significantly increases activity in the alpha frequency band which indicates that it relaxes the mind without inducing drowsiness. However, this effect has only been established at higher doses than that typically found in a cup of black tea (approximately 20mg). The aim of the current research was to establish this effect at more realistic dietary levels. EEG was measured in healthy, young participants at baseline and 45, 60, 75, 90 and 105 minutes after ingestion of 50mg L-theanine (n=16) or placebo (n=19). Participants were resting with their eyes closed during EEG recording. There was a greater increase in alpha activity across time in the L-theanine condition (relative to placebo (p+0.05). A second study replicated this effect in participants engaged in passive activity. These data indicate that L-theanine, at realistic dietary levels, has a significant effect on the general state of mental alertness or arousal. Furthermore, alpha activity is known to play an important role in critical aspects of attention, and further research is therefore focussed on understanding the effect of L-theanine on attentional processes. [3]


L-Theanine synergics effects with Caffeine.
The non-proteinic amino acid L-theanine and caffeine, a methylxanthine derivative, are naturally occurring ingredients in tea. The present study investigated the effect of a combination of 97 mg L-theanine and 40 mg caffeine as compared to placebo treatment on cognitive performance, alertness, blood pressure, and heart rate in a sample of young adults (n = 44). Cognitive performance, self-reported mood, blood pressure, and heart rate were measured before L-theanine and caffeine administration (i.e. at baseline) and 20 min and 70 min thereafter. The combination of moderate levels of L-theanine and caffeine significantly improved accuracy during task switching and self-reported alertness (both P < 0.01) and reduced self-reported tiredness (P < 0.05). There were no significant effects on other cognitive tasks, such as visual search, choice reaction times, or mental rotation. The present results suggest that 97 mg of L-theanine in combination with 40 mg of caffeine helps to focus attention during a demanding cognitive task. [4]

L-Theanine is an amino acid found naturally in tea. Despite the common consumption of L-theanine, predominantly in combination with caffeine in the form of tea, only one study to date has examined the cognitive effects of this substance alone, and none have examined its effects when combined with caffeine. The present randomised, placebo-controlled, double-blind, balanced crossover study investigated the acute cognitive and mood effects of L-theanine (250 mg), and caffeine (150 mg), in isolation and in combination. Salivary caffeine levels were co-monitored. L-Theanine increased 'headache' ratings and decreased correct serial seven subtractions. Caffeine led to faster digit vigilance reaction time, improved Rapid Visual Information Processing (RVIP) accuracy and attenuated increases in self-reported 'mental fatigue'. In addition to improving RVIP accuracy and 'mental fatigue' ratings, the combination also led to faster simple reaction time, faster numeric working memory reaction time and improved sentence verification accuracy. 'Headache' and 'tired' ratings were reduced and 'alert' ratings increased. There was also a significant positive caffeine x L-theanine interaction on delayed word recognition reaction time. These results suggest that beverages containing L-theanine and caffeine may have a different pharmacological profile to those containing caffeine alone. [5]

The aim of this study was to compare 50 mg caffeine, with and without 100 mg L-theanine, on cognition and mood in healthy volunteers. The effects of these treatments on word recognition, rapid visual information processing, critical flicker fusion threshold, attention switching and mood were compared to placebo in 27 participants. Performance was measured at baseline and again 60 min and 90 min after each treatment (separated by a 7-day washout). Caffeine improved subjective alertness at 60 min and accuracy on the attention-switching task at 90 min. The L-theanine and caffeine combination improved both speed and accuracy of performance of the attention-switching task at 60 min, and reduced susceptibility to distracting information in the memory task at both 60 min and 90 min. These results replicate previous evidence which suggests that L-theanine and caffeine in combination are beneficial for improving performance on cognitively demanding tasks. [6]

This review summarizes the literature on the association between two dietary components of tea, caffeine and L-theanine, and the psychological outcomes of consumption; it also identifies areas for future research. The studies reviewed suggest that caffeinated tea, when ingested at regular intervals, may maintain alertness, focused attention, and accuracy and may modulate the more acute effects of higher doses of caffeine. These findings concur with the neurochemical effects of L-theanine on the brain. L-theanine may interact with caffeine to enhance performance in terms of attention switching and the ability to ignore distraction; this is likely to be reflective of higher-level cognitive activity and may be sensitive to the detrimental effects of overstimulation. Further research should investigate the interactive effects of caffeine, L-theanine, and task complexity, utilize a range of ecologically valid psychological outcomes, and assess the neuroprotective effects of L-theanine using epidemiological or longer-term intervention studies among individuals at risk of neurodegenerative disease. [7]

L-Theanine safety
This study was conducted to evaluate the safety of l-theanine (Suntheanine) when administered as a dietary admixture to male and female Crl:CD (SD)GS BR rats at concentrations providing doses of 0, 1500, 3000 or 4000 mg/kg bw/day for 13 weeks. The study design was consistent with OECD Guideline 408 and USFDA Redbook II (1993) and GLP. There were no consistent, statistically significant treatment-related adverse effects on behavior, morbidity, mortality, body weight, food consumption and efficiency, clinical chemistry, hematology, or urinalysis. There were no consistent treatment-related adverse effects in gross pathology, organ weights or ratios or histopathology. The increased incidence of renal tubular cell adenomas in high-dose females only were not consistent with the characteristics of a renal carcinogen (due to early onset and low number of animals affected) but were more consistent with a genetic predisposition than with direct organ toxicity. The no-observed-adverse-effect-level (NOAEL) was 4000 mg/kg bw/day, the highest dose tested. [8]


L-Theanine Neuroprotection & Antigens
Human gammadelta T cells mediate innate immunity to microbes via T cell receptor-dependent recognition of unprocessed antigens with conserved molecular patterns. These nonpeptide alkylamine antigens are shared by tumor cells, bacteria, parasites, and fungi but also by edible plant products such as tea, apples, mushrooms, and wine. Here we show that priming of gammadelta T cells with alkylamine antigens in vitro results in a memory response to these antigens. Such priming results also in a nonmemory response to whole bacteria and to lipopolysaccharide, characterized by IL-12-dependent secretion of IFN-gamma by gammadelta T cells and by gammadelta T cell proliferation. Drinking tea, which contains l-theanine, a precursor of the nonpeptide antigen ethylamine, primed peripheral blood gammadelta T cells to mediate a memory response on reexposure to ethylamine and to secrete IFN-gamma in response to bacteria. This unique combination of innate immune response and immunologic memory shows that gammadelta T cells can function as a bridge between innate and acquired immunity. In addition, these data provide an explanation for the health benefits of tea. [9]

Several environmental neurotoxins and oxidative stress inducers are known to damage the nervous system and are considered major factors associated with the selective vulnerability of nigral dopaminergic neurons in Parkinson's disease (PD). Gamma-glutamylethylamide (L-theanine), a natural glutamate analog in green tea, has been shown to exert strong anti-ischemic effect. In this study, we investigated the protective effects of L-theanine on neurotoxicity induced by PD-related neurotoxicants, rotenone and dieldrin in cultured human dopaminergic cell line, SH-SY5Y. Our initial experiments revealed that L-theanine (500 microM) attenuated both rotenone- and dieldrin-induced DNA fragmentation and apoptotic death in SH-SY5Y cells. In addition, L-theanine partially prevented both rotenone- and dieldrin-induced heme oxygenase-1 (HO-1) up-regulation. Both rotenone- and dieldrin-induced down-regulation of extracellular signal-regulated kinase1/2 (ERK1/2) phosphorylation was significantly blocked by pretreatment with L-theanine. Furthermore, pretreatment with L-theanine significantly attenuated the down-regulation of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) production in SH-SY5Y cells. These results suggest that L-theanine directly provide neuroprotection against PD-related neurotoxicants and may be clinically useful for preventing PD symptoms. [10]

Amyloid beta (Abeta)-induced neurotoxicity is a major pathological mechanism of Alzheimer disease (AD). In this study, we investigated the inhibitory effect of l-theanine, a component of green tea (Camellia sinensis), on Abeta(1-42)-induced neuronal cell death and memory impairment. Oral treatment of l-theanine (2 and 4 mg/kg) for 5 weeks in the drinking water of mice, followed by injection of Abeta(1-42) (2 microg/mouse, icv), significantly attenuated Abeta(1-42)-induced memory impairment. Furthermore, l-theanine reduced Abeta(1-42) levels and the accompanying Abeta(1-42)-induced neuronal cell death in the cortex and hippocampus of the brain. Moreover, l-theanine inhibited Abeta(1-42)-induced extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase as well as the activity of nuclear factor kappaB (NF-kappaB). l-Theanine also significantly reduced oxidative protein and lipid damage and the elevation of glutathione levels in the brain. These data suggest that the positive effects of l-theanine on memory may be mediated by suppression of ERK/p38 and NF-kappaB as well as the reduction of macromolecular oxidative damage. Thus, l-theanine may be useful in the prevention and treatment of AD. [11]

As a natural analogue of glutamate, l-theanine is the unique amino acid derivative in green tea. Although its underlining mechanisms are not yet clear, it has been suggested that l-theanine treatment may prove beneficial to patients with neurodegenerative diseases. In this study, we investigated the neuroprotective effect and its mechanism of l-theanine in an in vitro model of Alzheimer's disease by using the human APP (Swedish mutation) transgenic SH-SY5Y cell. Amyloid beta (Abeta) neurotoxicity was triggered by l-glutamate in this cell line. Additionally, l-theanine significantly attenuated l-glutamate-induced apoptosis at similar levels to those seen with the NMDA receptor inhibitor MK-801 in the stably expressing APP Swedish mutation SH-SY5Y cells which over-generated Abeta. Meanwhile, the activation of c-Jun N-terminal kinase and caspase-3 induced by l-glutamate was suppressed by l-theanine. We also found that cells treated with l-theanine showed decreased production of nitric oxide resulting from the down-regulated protein levels of inducible nitric oxide synthase (iNOS) and neuronal nitric oxide synthase (nNOS). These results indicate that the inhibition of the NMDA subtype of glutamate receptors and its related pathways is the crucial point of the neuroprotective effect of l-theanine in the cell model. Thus, our present study supports the notion that l-theanine may provide effective prophylaxis and treatment for Alzheimer's disease. [12]

Edited by Ichoose2live, 01 January 2011 - 10:23 PM.

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#2 trismatic

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Posted 02 January 2011 - 12:19 PM

Nice topic. I've been interested in L-theanine since I started drinking green tea. Strong green tea gives me a very relaxed feeling, I can concentrate better. When I heard green tea has a lot of fluoride I ordered L-theanine powder. It has completely no noticiable effect regardless of the dosage. That could be the lack of caffeine :-)

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#3 nito

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Posted 06 January 2011 - 11:03 AM

what brand of l theanine do you take ichooe2live?

#4 SE102

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Posted 06 January 2011 - 11:36 AM

I do love L-Theanine
Especially with some DMAE-H3

(unless thats dumb)

Edited by SE102, 06 January 2011 - 11:36 AM.


#5 Ichoose2live

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Posted 06 January 2011 - 11:41 AM

what brand of l theanine do you take ichooe2live?


Any brand that is recognized to be Suntheanine. Currently I'm taking NOW L-Theanine.

#6 ^ risetolower v

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Posted 08 January 2011 - 06:47 AM

L-Theanine
L-theanine (N-ethyl-L-glutamine) or theanine is a major amino acid uniquely found in green tea. L-theanine has been historically reported as a relaxing agent, prompting scientific research on its pharmacology. Animal neurochemistry studies suggest that L-theanine increases brain serotonin, dopamine, GABA levels and has micromolar affinities for AMPA, Kainate and NMDA receptors. In addition has been shown to exert neuroprotective effects in animal models possibly through its antagonistic effects on group 1 metabotrophic glutamate receptors. Behavioural studies in animals suggest improvement in learning and memory. Overall, L-theanine displays a neuropharmacology suggestive of a possible neuroprotective and cognitive enhancing agent and warrants further investigation in animals and humans. [1]

L-theanine increases alpha wave activity in the occipital and pariental lobes of the brain, which I'm not sure if it's a good thing. The occipital-pariental area is involved in intelligence so wouldn't you want it to be active, not relaxed?

#7 Ichoose2live

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Posted 25 January 2011 - 01:57 AM

L-Theanine
L-theanine (N-ethyl-L-glutamine) or theanine is a major amino acid uniquely found in green tea. L-theanine has been historically reported as a relaxing agent, prompting scientific research on its pharmacology. Animal neurochemistry studies suggest that L-theanine increases brain serotonin, dopamine, GABA levels and has micromolar affinities for AMPA, Kainate and NMDA receptors. In addition has been shown to exert neuroprotective effects in animal models possibly through its antagonistic effects on group 1 metabotrophic glutamate receptors. Behavioural studies in animals suggest improvement in learning and memory. Overall, L-theanine displays a neuropharmacology suggestive of a possible neuroprotective and cognitive enhancing agent and warrants further investigation in animals and humans. [1]

L-theanine increases alpha wave activity in the occipital and pariental lobes of the brain, which I'm not sure if it's a good thing. The occipital-pariental area is involved in intelligence so wouldn't you want it to be active, not relaxed?


Alpha wave helps with Creativity. Creativity
I also find better thoughts processing when combined with Piracetam.
I asume that L-Theanine is Synergic with Piracetam because of the micromolar affinities on AMPA, NMDA and Kainate receptors. Piracetam also increase Alpha-wave. EEG Alpha-wave PIR EEG effects in the alpha- and beta-frequency ranges during the acute and subchronic administration of piracetam

#8 longevitynow

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Posted 25 January 2011 - 03:18 AM

I love theanine. As most capsules are 100 mgs, I never noticed much, even taking 2. Then I started experimenting with it in either higher doses (400-1000 mgs)and/or using it sublingually. I find it can be quite relaxing without sedating people. A friend had a job interview and was both anxious in general and especially anxious about the job interview. She took 1000 mgs before the interview, felt totally relaxed and totally sharp at the same time, and got the job.

#9 caruga

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Posted 25 January 2011 - 12:25 PM

It's the reverse for me. Anything above 150mg does nothing for me. At about 100mg I become more exuberent off of it. Interestingly, I only receive any calming effects if I took phenibut the previous day, upon which it becomes quite sedating, otherwise I experience no calming (or anxiolytic) effects.

#10 Ichoose2live

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Posted 21 February 2011 - 04:33 AM

I want to update my experience with L-Theanine. I use L-Theanine 30 minutes before going to bed. The effects are as I wanted.

- No sweat at night.

- No nightmares, which includes no throbbing so it's a big plus for the heart.

- Better facility to wake up the next day.

That's about it. I do not use it during the day because it makes me think less and with physical exercise it makes me mentally empty on all aspects (emotions, cognition, thoughts, etc.).

#11 Not2BUnlimited

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Posted 08 February 2012 - 08:14 PM

My first positive experience with L-Theanine was Gateraid Focus (the one with Tiger). I did half and half green tea and Gatoraid and the result was remarkable. Then Tiger got in trouble and my new favorite elixir was off the shelves. I contacted Gatoraid, but they couldn't tell me their source of LTheanine or how much was in the drink.

I've tried many (at least 3) brands of L-Theanine including Suntheanine and have never replicated the results I got from that drink.

Edited by Not2BUnlimited, 08 February 2012 - 08:33 PM.


#12 hippocampus

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Posted 08 February 2012 - 11:12 PM

How to reverse tolerance from theanine?

#13 earcaraxe

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Posted 09 February 2012 - 12:01 AM

I don't know that I'd call it "underrated" In fact, over on reddit at /r/nootropics everyone is talking about L-Theanine + Caffeine in combination. Kind of the current rage/trend whatever.

#14 panhedonic

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Posted 19 June 2012 - 05:52 PM

Just one observation regarding caffeine content in different types of tea:

"A Department of Nutritional Services report provides the following ranges of caffeine content for a cup of tea made with loose leaves:

Black Tea: 23 - 110 mg
Oolong Tea: 12 - 55 mg
Green Tea: 8 - 36 mg
White Tea: 6 – 25 mg
"

Green tea is surprisingly low. And white even lower! that explains why my white tea ingestion wasn't all that exciting.

#15 skypower

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Posted 19 June 2012 - 08:06 PM

Caffeine is probably the only substance which has so strong effect on me. I don't see any difference when I add theanine (up to 1000mg).

#16 panhedonic

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Posted 19 June 2012 - 08:37 PM

I have to say, I haven't had any chance to detect the effect of theanine myself, but I'm not giving up. Will try higher doses.

#17 MangekyōPeter

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Posted 22 June 2012 - 10:02 AM

I have to say, I haven't had any chance to detect the effect of theanine myself, but I'm not giving up. Will try higher doses.


same here, no matter if i switch brands or try different dosages, it just gives me brain fog if anything.. wouldn't call relaxator-ish at all..
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#18 Galaxyshock

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Posted 26 June 2012 - 12:24 PM

Theanine's primary effect is on GABA. If your brain has sufficient GABA activity the effects may be too subtle to notice and a high dose may just end up sedating/fogging. When the GABA activity is compromised because of some type of stress the theanine's effects can be really noticeable. Caffeine reduces GABA activity and this is part of the reason why theanine combines so well - healthy "upper-downer".
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#19 panhedonic

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Posted 26 June 2012 - 12:32 PM

Thanks, Galaxyshock. Now I understand. I'll reserve it for the mid-afternoon double-espresso, or for stressful times.

#20 darejz00

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Posted 08 August 2013 - 02:11 PM

What a clear and informative write-up, thank you! Do you know if the smaller caffeine doses of 50mg would cause tolerance or withdrawal issues if taken daily, say 5 days out of the week?

#21 chris106

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Posted 09 August 2013 - 10:18 AM

When I first tried L-Theanine a few months ago it was just unpleasantly sedating for me. So I wrote it off quite quickly.

Good thing i still kept my batch though. Cause as I later found out, it combines not only very well with Caffeine but with Modafinil as well!

I don't take Modafinil without L-Theanine anymore. It does a nice job in taking of the edge and even delaying tolerance for a few days. The same goes for Ashwagandha BTW - which makes sense, since it's a GABAergic, too.

I'll probably try cycling Modafinil + Ashwagandha with Caffeine + L-Theanine in the future.

Edited by chris106, 09 August 2013 - 10:19 AM.


#22 chris106

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Posted 09 August 2013 - 10:22 AM

Caffeine is probably the only substance which has so strong effect on me. I don't see any difference when I add theanine (up to 1000mg).


Maybe you should try Ashwagandha instead - it's GABAergic effects are moderately stronger than L-Theanine's, if I'm not mistaken.

#23 Sholrak

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Posted 09 August 2013 - 03:46 PM

Nice thread. Would be wise to take a cup of coffee and a cup of green tea? I have not L-theanine powders or capsules but I do own a bag of green tea.

Would be too much caffeine from the coffee plus the green tea? How much mg of each one caffeine and l-theanine is correct?

#24 GreenTeaFocus

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Posted 08 November 2013 - 12:00 AM

This post set the ground work for my business, which is awesome. I created a business called Green Tea Focus which sells the L-Theanine (200mg) + Caffeine (100mg) supplement in a singular capsule. Its little bit more convenient than making it yourself and decently priced. Check us out at greenteafocus.com!

#25 Godof Smallthings

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Posted 08 November 2013 - 03:21 AM

Nice thread. Would be wise to take a cup of coffee and a cup of green tea? I have not L-theanine powders or capsules but I do own a bag of green tea.

Would be too much caffeine from the coffee plus the green tea? How much mg of each one caffeine and l-theanine is correct?


The mix of l-theanine and caffeine in green tea itself is already very good. I don't think you need to add any coffee. But if you want to boost the effects of the green tea, I find that taking it with ginkgo biloba, piracetam and fish oil is a perfect combination.

#26 Neal Cullum

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Posted 05 December 2013 - 04:58 PM

Nice thread. Would be wise to take a cup of coffee and a cup of green tea? I have not L-theanine powders or capsules but I do own a bag of green tea.

Would be too much caffeine from the coffee plus the green tea? How much mg of each one caffeine and l-theanine is correct?


The mix of l-theanine and caffeine in green tea itself is already very good. I don't think you need to add any coffee. But if you want to boost the effects of the green tea, I find that taking it with ginkgo biloba, piracetam and fish oil is a perfect combination.


What's your experiences with taking ginkgo, puracetam and fish oil with green tea?

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#27 jroseland

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Posted 19 January 2021 - 10:05 AM

I frequently receive emails and Facebook messages asking me something to the effect of...
 
"What is the best Nootropic increasing social confidence?"
 
Near the top of my list of exotic molecules to achieve that is L-Theanine. in about 30 minutes it consistently brings me a measure of tranquility to manage social situations with grace and gravitas. As the active ingredient in green tea, it's an exceptionally safe nutraceutical, with virtually no downside.
 
6eba23a5547ce767.jpg
 





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