Great to get in touch with stem cell enthusiasts and would love to learn more about the subject from amazing LGcitizens.   

One question:

From what I read, injecting stem cells has sort of been norm in repairing ligament inguries. Regular treatment is to inject MSCs and HSCs into joints and wait till they differentiate. Do I get it right?

But to my astonishment, the other day a biology blogger told me the role of MSCs in ligament repairment is NOT to differentiate. Also, according to what he said, "In ligament repairment, MSCs are injected along with HA（hyaluronic acid），instead of HSCs...The real role of MSCs injected into joints is to inhibit inflammation and activate dormant stem cells already existed in joints to differentiate...". Shocking thing to know!!!!

Since I'm not a biology major and all my limited understandings of stem cells come from internet. It's beyond my ability to tell if the blogger is right or wrong. Any pro out there can help me with this?

The answer is meaningful to me 'cause I broke my shoulder joint and am supposed to have an arthroscopy. But my allergic asthma could pose a huge threat to my life under general anesthesia so I'm trying my best to avoid having any type of surgery. IF MSCs's role in ligament repairment, as the blogger said, is  to inhibit inflammation and activate dormant stem cells already existed in joints. I think I have other options to achieve the same goal.

Just want to know if the blogger is right or wrong in the workings of MSCs. No HSCs needed in ligament repairment? Hard to believe. Very different from what I read previously. BUT if he was right, it's goog news to me.

Many thanks in advance. 

Winnie

First I was kind of overwhelmed with the requirements, time and money needed for trying to replicate and/or incorporate the protocol in my own regimen. Then I started to feel it's actually a challenge .

I've therefore started to draft a regimen based on the TRIIM Protocol which I think I might be able to start in a month or so. Currently I'm collecting the necessary substances and tests needed. I've excluded HGH (Omnitrope) due to its lack of easy availability and will instead include some other substances which I think might be beneficial. From a testing point of view there will be no MRI's but some other tests with an emphasis on telomeres.

Because the the modified protocol is kind of ambitious I wonder if you see any obvious mistakes or easy improvements to it.

Below I've added is my first draft.

TRIIM Protocol without HGH and with some additional modifications

HGH has been left out because it seem to be a bit hard to obtain.

Pregnenolone has been included on a hunch.

Astragalus has been included since I'm currently using it (baseline/what I'm using today: once per day in the morning).

Gingko Biloba has been included since I'm currently using it (baseline: 1-3 times per week, usually around lunch).

Besides the substances below I consider the following to be included:

- Training sessions 2-3 times per week (baseline: currently 1 time per week)

- Regular saunas 4-5 times per week in order to decrease heavy metals (baseline: 1 time per half year)

- Transcendental Meditation at least 4-5 times per week around lunch (baseline: non-existent)

- Lower environmental stress (baseline: quite high)

- Low Carb High Fat diet (baseline: I'm currently in ketosis only one week every month or so)

1st week

Baseline tests

• Baseline blood tests: "Healthcheck XL+" with added Zinc, Cortisol, DHEAS, Thyroid tests. All tests during the morning.
• Baseline blood test: myDNAge Epigenetic Aging Clock test, around lunch
• Baseline blood test: RepeatDX 6-Panel assay Telomere length test, around lunch.
• Baseline blood test: LifeLength TAT, around lunch
• Baseline Saliva tests: Cortisol (morning), DHEA (morning)
• Baseline Heavy Metal Urine Test: 17 substances (Aluminum, Antimony, Arsenic, Lead, Cadmium, Chromium, Cobalt, Copper, Nickel, Palladium, Mercury, Iron, Platinum, Silver, Thallium, Zinc and Pewter), morning.
• uBiome Five Site bacterial tests: Gut, Mouth, Nose, Genitals, Skin)

Morning

1 x 25 mg DHEA

1 x 30 mg Pregnenolone

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

Lunch

1x 105 mg Gingko Biloba

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

2nd week

Adjustment tests

Blood

• CRP, Hb, LPK, TPK, MCH, MCHC, MCV, EPK, EVF
• DHEAS
• HbA1c (glycated hemoglobin)
• Glucosis
• C-Peptide

Saliva

• Cortisol (morning), DHEA (morning)

Morning

1 x 25 mg DHEA

1 x 30 mg Pregnenolone

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

Lunch

1x 105 mg Gingko Biloba

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

3rd week

Morning

2 x 25 mg DHEA (dose change to 50 mg if adjustment test results support this)

1 x 100 mg Pregnenolone (dose change to 100 mg if adjustment test results support this)

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

Lunch

1 x 500 mg Glucophage (Metformin)

1x 105 mg Gingko Biloba

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

4th week

Adjustment tests

Blood

• CRP, Hb, LPK, TPK, MCH, MCHC, MCV, EPK, EVF
• DHEAS
• HbA1c (Glycated hemoglobin)
• Glucosis
• C-Peptide

Saliva

• Cortisol (morning), DHEA (morning)

Morning

2 x 25 mg DHEA (dose change if adjustment test results support this)

1 x 100 mg Pregnenolone (dose change if adjustment test results support this)

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

Lunch

1 x 500 mg Glucophage (Metformin)

1x 105 mg Gingko Biloba

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

5th to 52nd week

Morning

1 x 25 mg DHEA

1 x 30 mg Pregnenolone

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

Lunch

1 x 500 mg Glucophage (Metformin)

1 x 105 mg Gingko Biloba

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

Week 53

Tests for results

• Blood tests: Healthcheck XL+ with added Zinc, Cortisol, DHEAS, Thyroid tests. All tests during the morning.
• Blood test: myDNAge Epigenetic Aging Clock test, around lunch.
• Blood tests: RepeatDX 6-Panel assay Telomere length test, around lunch.
• Blood test: LifeLength TAT, around lunch.
• Saliva tests: Cortisol (morning), DHEA (morning)
• Heavy Metal Urine Test: 17 substances  (Aluminum, Antimony, Arsenic, Lead, Cadmium, Chromium, Cobalt, Copper, Nickel, Palladium, Mercury, Iron, Platinum, Silver, Thallium, Zinc and Pewter), morning.
• uBiome Five Site bacterial tests: Gut, Mouth, Nose, Genitals, Skin

So, I have health problems. I have Autoimmune problems with Hashimoto's, Alopecia, Eczema. I also tested positive for Bartonella and Epstein Barr Virus recently (negative for Lyme though).

I'm a 31 year old male, and I have been sick for over 5 years, since early 2014. My main problems are fatigue, inability to handle stress, anxiety, and brain fog. Also, I tend to have a tired but wired feeling, and haven't been sleeping well lately.

I have tried a bunch of supplements, and take many now (I have listed the supplements I take at this google sites website: https://sites.google.com/view/hashimotosbartonellasupplement). I have used the website "selfdecode.com", to upload my 23andMe results, and get personalized recommendations based on my genetics. I came across a SelfDecode blog article that talked about brain histamine levels, related to the gene HMNT (specifically the SNP "rs1050891"), and the enzyme histamine-N-methyltransferase, which breaks down histamine in the brain. This is the article:  https://www.selfdecode.com/blog/article/brain-histamine-hnmt-13. 

My result for the SNP was homozygous "AA", which is the risk allele, and causes less breakdown of histamine by the enzyme, so can increase brain histamine. The blog article said that theanine and SAM-e can help with this. I already take theanine, and like its effects, although they are quite subtle at this point, as I've been taking theanine for about 9 months. But the article said that since SAM-e is a methyl donor and the enzyme HNMT inactivates histamine by methylating it, SAM-e may help HNMT work better to decrease brain histamine. 

So I ordered a bottle of SAM-e. I took one capsule of 200mg of SAM-e (from the brand Pure Encapsulations) yesterday and today. I can definitely feels the effects from it, seemingly about 30 minutes after taking it. The first night I took it, I felt pretty sleepy right after taking it, like a comfortable and relieving type of sleepy feeling. This feeling doesn't happen much anymore, and I welcome it. But I also felt a bit loopy and out of it from taking the SAM-e. The second day was similar. The second day I took the SAM-e in the afternoon (compared to the evening on the first day). I got comfortably relaxed after taking it, but then I went to work, which is DoorDash delivery currently. I noticed that while the SAM-e made me less anxious while waiting for the food in the restaurant, and I might have felt more comfortable talking to the other people waiting with me, I felt a little loopy, and like I had lost some where-with-all and alertness. I had lost some of that inner dialogue that makes me feel in control and alert, but can also contribute to feelings of anxiety. So I was in a bit of a daze. 

Usually I just feel really stressed, and like my brain is inflamed, so this relatively comfortable loopiness is still not completely hated by me, but I'm wondering why I feel like this, since many people report feeling more alert and have more energy from SAM-e. I know that SAM-e helps increase neurotransmitters like acetylcholine, dopamine, serotonin, norepinephrine and melatonin. So maybe it is activating serotonin or melatonin more for me?

Or maybe it is decreasing brain histamine, and the loopiness is a temporary side-effect?

I've also heard that SAM-e can cause mania in people who are bipolar. I'm not bipolar, but was wondering if there was any relation here, with my experience with SAM-e.

Anyway, I'm writing and asking this because I can't really tell if these effects from SAM-e are good or bad, worrisome or not. I may try it again tomorrow, I'm not sure. Or maybe next time I'll just take it right before I go to bed, and see how I feel in the morning.

So, I have health problems. I have Autoimmune problems with Hashimoto's, Alopecia, Eczema. I also tested positive for Bartonella and Epstein Barr Virus recently (negative for Lyme though).

I'm a 31 year old male, and I have been sick for over 5 years, since early 2014. My main problems are fatigue, inability to handle stress, anxiety, and brain fog. Also, I tend to have a tired but wired feeling, and haven't been sleeping well lately.

I have tried a bunch of supplements, and take many now (I have listed the supplements I take at this google sites website: https://sites.google.com/view/hashimotosbartonellasupplement). I have used the website "selfdecode.com", to upload my 23andMe results, and get personalized recommendations based on my genetics. I came across a SelfDecode blog article that talked about brain histamine levels, related to the gene HMNT (specifically the SNP "rs1050891"), and the enzyme histamine-N-methyltransferase, which breaks down histamine in the brain. This is the article:  https://www.selfdecode.com/blog/article/brain-histamine-hnmt-13. 

My result for the SNP was homozygous "AA", which is the risk allele, and causes less breakdown of histamine by the enzyme, so can increase brain histamine. The blog article said that theanine and SAM-e can help with this. I already take theanine, and like its effects, although they are quite subtle at this point, as I've been taking theanine for about 9 months. But the article said that since SAM-e is a methyl donor and the enzyme HNMT inactivates histamine by methylating it, SAM-e may help HNMT work better to decrease brain histamine. 

So I ordered a bottle of SAM-e. I took one capsule of 200mg of SAM-e (from the brand Pure Encapsulations) yesterday and today. I can definitely feels the effects from it, seemingly about 30 minutes after taking it. The first night I took it, I felt pretty sleepy right after taking it, like a comfortable and relieving type of sleepy feeling. This feeling doesn't happen much anymore, and I welcome it. But I also felt a bit loopy and out of it from taking the SAM-e. The second day was similar. The second day I took the SAM-e in the afternoon (compared to the evening on the first day). I got comfortably relaxed after taking it, but then I went to work, which is DoorDash delivery currently. I noticed that while the SAM-e made me less anxious while waiting for the food in the restaurant, and I might have felt more comfortable talking to the other people waiting with me, I felt a little loopy, and like I had lost some where-with-all and alertness. I had lost some of that inner dialogue that makes me feel in control and alert, but can also contribute to feelings of anxiety. So I was in a bit of a daze. 

Usually I just feel really stressed, and like my brain is inflamed, so this relatively comfortable loopiness is still not completely hated by me, but I'm wondering why I feel like this, since many people report feeling more alert and have more energy from SAM-e. I know that SAM-e helps increase neurotransmitters like acetylcholine, dopamine, serotonin, norepinephrine and melatonin. So maybe it is activating serotonin or melatonin more for me?

Or maybe it is decreasing brain histamine, and the loopiness is a temporary side-effect?

I've also heard that SAM-e can cause mania in people who are bipolar. I'm not bipolar, but was wondering if there was any relation here, with my experience with SAM-e.

Anyway, I'm writing and asking this because I can't really tell if these effects from SAM-e are good or bad, worrisome or not. I may try it again tomorrow, I'm not sure. Or maybe next time I'll just take it right before I go to bed, and see how I feel in the morning.