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[brian1965]

I thought the group might find this interesting:

"Scientists have moved one step closer to RNA editing, which could be the next stage of CRISPR"

https://techcrunch.c...tage-of-crispr/

Main scientific references:
https://www.cell.com...8674(18)31173-5
https://www.nature.c...ticles/Nbt.4192

From the Cell article (Vol 175, Issue 1, pp 212-223.E17, Sep 20, 2018):

Highlights

• Structures of the smallest type VI CRISPR effector in guide and target-bound states
• Mechanistic insights into guide RNA and target RNA recognition
• Insights into apo Cas13d structural dynamics through cryo-EM and HDX-MS
• Rational engineering of Cas13d for minimal coding sequence

Summary

CRISPR-Cas endonucleases directed against foreign nucleic acids mediate prokaryoticadaptive immunity and have been tailored for broad genetic engineering applications.Type VI-D CRISPR systems contain the smallest known family of single effector Casenzymes, and their signature Cas13d ribonuclease employs guide RNAs to cleave matchingtarget RNAs. To understand the molecular basis for Cas13d function and explain itscompact molecular architecture, we resolved cryoelectron microscopy structures ofCas13d-guide RNA binary complex and Cas13d-guide-target RNA ternary complex to 3.4and 3.3 Å resolution, respectively. Furthermore, a 6.5 Å reconstruction of apo Cas13dcombined with hydrogen-deuterium exchange revealed conformational dynamics that haveimplications for RNA scanning. These structures, together with biochemical and cellularcharacterization, provide insights into its RNA-guided, RNA-targeting mechanism anddelineate a blueprint for the rational design of improved transcriptome engineeringtechnologies.