9 replies to this topic

[stefan_001]

This detailed study seems to have gone under the radar:

Nicotinamide riboside regulates inflammation and mitochondrial markers in AML12 hepatocytes

https://synapse.kore...nrp.2019.13.1.3

This study suggests the potential role of NR in inflammation regulation and mitochondrial biogenesis in a model of hepatocyte steatosis. As inflammation and mitochondrial dysfunction are involved in the pathophysiology in NAFLD, our study has potential clinical and public health implications if the findings are confirmed in human clinical trials. In conclusion, our findings demonstrate that NR treatment attenuates hepatic inflammation, and induces mitochondrial biogenesis in mouse hepatocytes. These findings suggest the therapeutic value of NR in hepatic inflammation and impaired mitochondrial biogenesis.

 

 

 

[Phoebus]

wow, NR actually did quite a lot of good things in that study, anti cancer ant inflammatory,  Sirt1 and Sirt3 upregulated etc

 

 

 

 Exposure to NR had no effect on cell viability or morphology. Gene expression of sirtuin 1 (Sirt1) and Sirt3 was significantly upregulated by NR in PA-treated hepatocytes. However, Sirt1 activities were increased in hepatocytes treated with low-dose NR. Hepatic pro-inflammatory markers including tumor necrosis factor-alpha and interleukin-6 were decreased in NR-treated cells. NR upregulated anti-inflammatory molecule adiponectin, and, tended to down-regulate hepatokine fetuin-A in PA-treated hepatocytes, suggesting its inverse regulation on these cytokines. NR increased levels of mitochondrial markers including peroxisome proliferator-activated receptor γ coactivator-1α, carnitine palmitoyltransferase 1, uncoupling protein 2, transcription factor A, mitochondrial and mitochondrial DNA in PA-treated hepatocytes.

 

[Hebbeh]

I doubt in vitro mouse cells in a petri dish carries over to a human in vivo model in any meaningful way.  I wouldn't draw any real life conclusions from this type of test tube experiment.

[stefan_001]

I doubt in vitro mouse cells in a petri dish carries over to a human in vivo model in any meaningful way.  I wouldn't draw any real life conclusions from this type of test tube experiment.

 

not just petri dish:

https://www.cell.com...4)00392-4?cc=y=

[Hebbeh]

not just petri dish:

https://www.cell.com...4)00392-4?cc=y=

 

This is a different study and off topic to the thread and quoted comment.  Regardless, your 2nd study in the quote is still on not only mice but "genetically engineered" (read genetically FU) mice which were genetically manipulated to provide a desired result.  Even under the best of circumstances using wild type mice, rarely if ever do results transfer over to a real life human model.  Mice just do not make a relevant test subject...unless you are designing for mice.  I doubt you can find any type of mice studies which have ever translated over to the human model.  Unfortunately, this is only good news...if you are a mouse with an intentionally FU liver.

[stefan_001]

This is a different study and off topic to the thread and quoted comment.  Regardless, your 2nd study in the quote is still on not only mice but "genetically engineered" (read genetically FU) mice which were genetically manipulated to provide a desired result.  Even under the best of circumstances using wild type mice, rarely if ever do results transfer over to a real life human model.  Mice just do not make a relevant test subject...unless you are designing for mice.  I doubt you can find any type of mice studies which have ever translated over to the human model.  Unfortunately, this is only good news...if you are a mouse with an intentionally FU liver.

 

Long time huh since you opened disucssions about those petri dish studies yourself....must have been a big hangover the resveratrol hype but I would recommend to nevertheless keep a bit open mind when research get published:

https://www.longecit...mmune-function/

Resveratrol boosts immune function
Started by Hebbeh, Sep 18 2013 02:32 AM

....The findings were made in laboratory cell cultures.........

 

Edited by stefan_001, 23 February 2019 - 07:33 PM.

[Hebbeh]

Long time huh since you opened disucssions about those petri dish studies yourself....must have been a big hangover the resveratrol hype but I would recommend to nevertheless keep a bit open mind when research get published:

https://www.longecit...mmune-function/

Resveratrol boosts immune function
Started by Hebbeh, Sep 18 2013 02:32 AM

....The findings were made in laboratory cell cultures.........

 

Obvious if you don't have a valid argument then attack the messenger….with a quoted study from 6 years ago no less.  And for the record, the quoted study at least wasn't in mice and I never made any self promoting (stock pumping) claims in regard to the study.  Touche though.

 

Edit: What would impress me would be a reference to any mouse study that has ever led to any medical break through.

Edited by Hebbeh, 23 February 2019 - 07:44 PM.

[stefan_001]

Edit: What would impress me would be a reference to any mouse study that has ever led to any medical break through.

 

Well for example cancer immunotherapies. The PD-1 blocking anti-cancer effect which I think its one of the real breakthrough for certain type of cancers and has saved many peoples lives.

Attached Files

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Edited by stefan_001, 23 February 2019 - 08:43 PM.

[Hebbeh]

Well for example cancer immunotherapies. The PD-1 blocking anti-cancer effect which I think its one of the real breakthrough for certain type of cancers and has saved many peoples lives.

 

Good point.  Congrats, well done.

[stefan_001]

Good point. Congrats, well done.

Thank you, appreciated. Have a good weekend.