• Log in with Facebook Log in with Twitter Log In with Google      Sign In    
  • Create Account
  LongeCity
              Advocacy & Research for Unlimited Lifespans

Photo
- - - - -

Nicotinamide riboside protects against liver fibrosis induced by CCl4 via regulating the acetylation of Smads signaling

liver

  • This topic is locked This topic is locked
No replies to this topic

#1 Fredrik

  • Guest
  • 493 posts
  • 89
  • Location:Stockholm, Sweden
  • NO

Posted 01 April 2019 - 09:39 PM


 

Nicotinamide riboside protects against liver fibrosis induced by CCl4 via regulating the acetylation of Smads signaling pathway.

 

Jiang R1, Zhou Y2, Wang S2, Pang N2, Huang Y2, Ye M2, Wan T2, Qiu Y2, Pei L2, Jiang X2, Huang Y3, Yang H3, Ling W2, Li X3, Zhang Z4, Yang L5.

 
Abstract
AIMS:

Increasing nicotinamide adenine dinucleotide (NAD+) by Nicotinamide riboside (NR) provides protective benefits in multiple disorders. However, the role of NR on liver fibrosis is unclear. We performed in vivo and in vitro experiments to test the hepatic protective effects of NR against liver fibrosis and the underlying mechanisms.

 

MATERIALS AND METHODS:

Mice were injected with CCl4 to establish liver fibrosis model. NR was given by gavage to explore the hepatic protection of NR. LX-2 cells were given a TGF-β stimulation ± NR, the activation of LX-2 cells and the acetylation of Smads were analyzed. To further confirm the role of Sirt1 on the protective pathway of NR, we knockdown Sirt1 in LX-2 cells.

 

KEY FINDINGS:

We found NR could prevent liver fibrosis and reverse the existing liver fibrosis. NR inhibited the activation of LX-2 cells induced by TGF-β, activated Sirt1 and deacetylated Smad2/3. Sirt1 knockdown diminished the inhibiting effect of NR on LX-2 cells activation, and increased expressions of acetylated Smads. In conclusion, NR could prevent liver fibrosis via suppressing activation of hepatic stellate cells (HSCs). This protective effect was mediated by regulating the acetylation of Smads signaling pathway.

 

SIGNIFICANCE:

NR protected mice against liver fibrosis induced by CCl4. NR suppressed activation of hepatic stellate cells induced by TGF-β. NR protects liver fibrosis via increasing the activity of Sirt1 and decreasing the expression of P300, resulting in the deacetylation of Smads in stellate cells.

 


Edited by Fredrik, 01 April 2019 - 09:42 PM.

  • Informative x 3





Also tagged with one or more of these keywords: liver

0 user(s) are reading this topic

0 members, 0 guests, 0 anonymous users