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Alpha-GPC Cytotoxic in Rat Myocytes

a-gpc alpha-gpc

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#1 ta5

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Posted 15 July 2019 - 04:34 AM

Mol Cell Biochem. 2019 Jul 6. Mol Cell Biochem. 2019 Jul 6.

L-Alpha-glycerylphosphorylcholine can be cytoprotective or cytotoxic in neonatal rat cardiac myocytes: a double-edged sword phenomenon.

L-Alpha-glycerylphosphorylcholine (GPC) is a widely used food supplement. GPC has been shown to exert beneficial effects in several organs; however, the cardiac effects of GPC have yet to be investigated. The aim of the present study was therefore to map out the effects of GPC on cardiac myocytes, with or without ischemia-reperfusion insult. Neonatal rat cardiac myocytes were treated with GPC at 1, 10, 80, and 100 µM concentrations for 15 min, 3 h, or 24 h, respectively. Cell viability by calcein assay and the degree of oxidative stress by DHE (superoxide level) and H2DCF (total ROS accumulation) staining were measured. In separate experiments, cardiomyocytes were pre-treated with the optimal concentration of GPC for 3 h and then cells were exposed to 4 h of simulated ischemia followed by 2 h of reperfusion (SI/R). Cell viability was measured at the end of the SI/R protocol. In normoxic conditions, the 15-min and the 3-h GPC treatment did not affect cell viability, total ROS, and superoxide levels. Under SI/R conditions, the 3-h GPC treatment protected the cardiac myocytes from SI/R-induced cell death and did not alter the level of oxidative stress. The 24-h GPC treatment in normoxic conditions resulted in significant cell death and increased oxidative stress at each concentration. Here we provide the first evidence for the cytoprotective effect of short-term GPC treatment. However, long-term administration of GPC may exert cytotoxicity in a wide concentration range in cardiac myocytes. These results may draw attention to a comprehensive cardiac safety protocol for the testing of GPC.
PMID: 31280435
I was disappointed to see this since I had switched to a-GPC from Citicoline (CDP-Choline) after finding Uridine might do some bad things.

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#2 prunk

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Posted 15 July 2019 - 12:35 PM

Well this sucks, I just love A-GPC

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#3 BasicBiO

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Posted 02 August 2019 - 01:17 AM

Keep in mind this is a test tube study done on this specific tissue line under ischemia-reperfusion insult for an entire 24 hours. I highly doubt your heart tissues would be exposed to a sustained concentration of GPC for 24 hours straight, under these conditions and you'd probably be dead from the ischemia, not the supplement.


I believe CoQ-10 has this same tendency, but I hedge my bets and take it anyway.



Also tagged with one or more of these keywords: a-gpc, alpha-gpc

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