16 replies to this topic

[Phoebus]

 

 

Int J Biol Sci 2020; 16(5):849-858. doi:10.7150/ijbs.40886

Research Paper

Improvement of cognitive and motor performance with mitotherapy in aged mice

Zizhen Zhao¹, Zhenyao Yu¹, Yixue Hou¹, Le Zhang², Ailing Fu¹ 

1. School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
2. College of Computer Science, Sichuan University, Chengdu, 610065, China.

 

 

Abstract

Changes in mitochondrial structure and function are mostly responsible for aging and age-related features. Whether healthy mitochondria could prevent aging is, however, unclear. Here we intravenously injected the mitochondria isolated from young mice into aged mice and investigated the mitotherapy on biochemistry metabolism and animal behaviors. The results showed that heterozygous mitochondrial DNA (mtDNA) of both aged and young mouse coexisted in tissues of aged mice after mitochondrial administration, and meanwhile, ATP content in tissues increased while reactive oxygen species (ROS) level reduced. Besides, the mitotherapy significantly improved cognitive and motor performance of aged mice. Our study, at the first report in aged animals, not only provides a useful approach to study mitochondrial function associated with aging, but also a new insight into anti-aging through mitotherapy.

 

 

https://www.ijbs.com/v16p0849.htm

 

full study at link 

[Phoebus]

 

 

Mitochondrial therapy (mitotherapy) is to transfer functional exogenous mitochondria into mitochondria-defective cells for recovery of the cell viability and consequently, prevention of the disease progress.

 

 

I honestly had no idea something like this could be done. 

 

Could you do this in humans? 

 

 

 

The mitochondria were isolated from young mouse liver. 

 

 

would that be possible to do with humans without extreme discomfort? 

Edited by Phoebus, 22 February 2020 - 03:00 PM.

[Fredrik]

Why is this posted under "SUPPLEMENTS" and the "NAD+" section of Longecity?

Edited by Fredrik, 22 February 2020 - 08:41 PM.

[orion22]

wow i wonder how much would that cost billions or $ trillions to replace all mitocondria in a human?

[Phoebus]

wow i wonder how much would that cost billions or $ trillions to replace all mitocondria in a human?

 

 

Why would it cost that much is you could harvest the mito from young people? 

[Fredrik]

This post should be transferred to the biotechnology section of the forum. It has nothing to do with either NAD+ or supplementation.

[Turnbuckle]

This injection experiment suggests that free floating mitochondria in the blood is there for a purpose--to seed cells that have had significant damage to their mitochondria with potentially better mitochondria. When the mito  damage is systemic, however, it is necessary to take aggressive measures to  clean up defective mitochondria. This is where NAD+ comes in, as cycling the NAD+/NADH ratio with mito fusion will set QC mechanisms running, destroying defective mitochondria while expanding the proportion of good mitochondria.

Edited by Turnbuckle, 23 February 2020 - 09:00 AM.

[Engadin]

This post should be transferred to the biotechnology section of the forum. It has nothing to do with either NAD+ or supplementation.

 

 

Done.

[Phoebus]

This injection experiment suggests that free floating mitochondria in the blood is there for a purpose--to seed cells that have had significant damage to their mitochondria with potentially better mitochondria. When the mito  damage is systemic, however, it is necessary to take aggressive measures to  clean up defective mitochondria. This is where NAD+ comes in, as cycling the NAD+/NADH ratio with mito fusion will set QC mechanisms running, destroying defective mitochondria while expanding the proportion of good mitochondria.

 

Right but the question is this - is IV mito therapy far more effective than IV NAD+ at revitalizing the hosts mito? 

[Turnbuckle]

Right but the question is this - is IV mito therapy far more effective than IV NAD+ at revitalizing the hosts mito? 

 

 

If you have very few good mitochondria, then IV would be best. But you will still have to multiply them and delete the bad ones. Otherwise you will only experience a very partial recovery.

[MikeDC]

This is probably one of the reasons young blood rejuvenate old.

[Phoebus]

This is probably one of the reasons young blood rejuvenate old.

  

 

could be, but I was under the impression that ultimately young blood infusions proved worthless in humans? 

[Turnbuckle]

Blood transfusions will transfer not only mitochondria, but also VSELs -- very small embryonic-like stem cells. These circulate in the blood and are apparently small enough to infiltrate any tissue. This paper examined the levels of VSELs in blood from human subjects of various ages. They say they didn't see a statistically significant fall off, but  Fig. 2 certainly shows one. That figure also shows that there is a large range of circulating VSELs depending on the individual, thus the likelihood of getting large amounts from "young blood" depends on the donor.

 

The size of VSELs is just above 7 µm, so their presence will be dependent on the filter used on the donor blood.

Attached Files

•  VSEL.PNG   152.26KB   0 downloads

Edited by Turnbuckle, 24 February 2020 - 12:10 PM.

[MikeDC]

I have heard people with high blood pressure becomes normal after blood infusion and the effects stay for a few months.

[Daniel Cooper]

There is a company (who's name escapes me at that moment - sorry) that is working on generating artificial mitochondria for just this reason.  There's other research out there that demonstrates that decreasing number and quality of mitochondria contribute to aging.  We've had clues in this direction for a while now.

 

 

[smithx]

Daniel Cooper please find the company. I want to invest in them

[Daniel Cooper]

Daniel Cooper please find the company. I want to invest in them

 
I'll keep looking.  Unfortunately when you Google "artificial mitochondria" you get a lot of research on artificial mitochondria (which is very interesting and worth a look) and a fair number of hits on using artificial intelligence to do research on mitochondria.  
 
Trying to remember where I saw the information.  Maybe on Josh Mitteldorf's site?
 
In the mean time - Artificial Mitochondria Transfer: Current Challenges, Advances, and Future Applications