38 replies to this topic

[sensei]

I would caution making statements regarding the elimination half-life of fisetin in humans.

The available pharmacokinetics data of a biphasic half-life of .9 and roughly 3 hours was measured in MICE not HUMANS. It may or may not be similar.

Caution is also warranted in that it has an IC80 of 100umol for the CYP3A4 enzyme.

https://www.research..._fig6_312187277

[FlorianReicht]

I'll give this a try. Adding some DMSO to the mixture for better absorption.

[nhenderson]

How about cocoa powder as a source of PCC1? given this

 

http://phenol-explor.../polyphenol/157

 

Also, two more questions 

1. Any thoughts about Life Extension's "Synolytic Activator" compound, just upping the dose?
2. Would dissolving the original protocol in full fat yogurt overnight be useful?

[Fafner55]

 

How about cocoa powder as a source of PCC1? given this

 

http://phenol-explor.../polyphenol/157

 

Also, two more questions 

1. Any thoughts about Life Extension's "Synolytic Activator" compound, just upping the dose?
2. Would dissolving the original protocol in full fat yogurt overnight be useful?

 

 

Great finds!

In my protocol I assumed that the PCC1 content of grape seed extract was 3%, based on

“Polyphenolic Composition and Antioxidant Activities of Grape Seed Extract” (2008) https://www.tandfonl...42910701584260 

while the reference cited by the phenol-explorer website give a similar result (2.63%).

“Levels of flavan-3-ols in French wines” (1999) https://doi.org/10.1...021/jf9810564  

 

If the 2.63% figure is used, the GSE dose would need to be increased from 4000 mg to 4300 mg. 

 

Cocoa powder could be a good substitute for GSE, but I'm skeptical of the 23% to 26% PCC1 content that http://phenol-explor.../polyphenol/157 reports. The original paper states a 2.58% recovery in Table 1. “Analyses of polyphenols in cacao liquor, cocoa, and chocolate by normal-phase and reversed-phase HPLC” (2020) https://academic.oup...2?login=false  

 

As for Life Extension's "Synolytic Activator", it is advertised as a "Proprietary Blend". Life Extension's website does not offer any quantitative measures of the effectiveness of Synolytic Activator. It only gives testimonials. That's marketing, not science. In contrast, the detail and support I put in the protocol document gives readers what they need to make fact-based decisions.

[FrankEd]

Fafner55, if you don´t mind, which brand of Fisetin and GSE you used in your protocol?

[Blueflash]

I've tried the first 3 days half dose. The first day I felt some fatigue, mild flu feeling. The second day at half dose I felt nothing. Today I did the full dose and again I felt the fatigue. Thanks for putting this together. Had been wanting to try something similar from the mayo clinic. 

[Fafner55]

I've tried the first 3 days half dose. The first day I felt some fatigue, mild flu feeling. The second day at half dose I felt nothing. Today I did the full dose and again I felt the fatigue. Thanks for putting this together. Had been wanting to try something similar from the mayo clinic. 

 

I also experienced mild flu-like feelings when first-time removing senescent cells: minor aches and a low grade fever. Now I only experience slight fatigue.

[Woody42]

I just mixed 4G of GSE with 2G of Fisetin in 30 ml of olive oil with 10 ml of lecithin and 100 ml of water.

Man did it tast bad !   Anyone have any further thoughts about just using cocoa powder instead of the

GSE perhaps it would be easier to swallow.

 

[Fafner55]

GSE powder tastes terrible. Try buying capsules.