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Frozen human brain tissue works perfectly when thawed 18 months later (MEDY)

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#1 MaximilianKohler

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Posted 18 May 2024 - 07:50 AM


https://newatlas.com...yopreservation/

 

Effective cryopreservation of human brain tissue and neural organoids (May 2024) https://www.cell.com...2375(24)00121-8

 

 

 

the researchers arrived at the most promising mixture, which they called MEDY, after the four main ingredients: methylcellulose, ethylene glycol, DMSO and Y27632.

 

 

 

Highlights
  • We establish a cryopreservation method (MEDY) for brain organoids and tissues
  • MEDY maintains the structure and functional activity of cortical organoids
  • MEDY can be applied to diverse brain organoids, including patient-derived organoids
Motivation
Brain organoids are useful models for modeling brain diseases. However, long-term culture, high costs, and storage problems significantly limit their application in biomedical studies. Here, we establish a method using methylcellulose, ethylene glycol, DMSO, and Y27632 (termed MEDY) for the cryopreservation of diverse brain organoids or human brain tissue without disrupting the neural cytoarchitecture or functional activity.
Summary
Human brain tissue models and organoids are vital for studying and modeling human neurological disease. However, the high cost of long-term cultured organoids inhibits their wide-ranging application. It is therefore urgent to develop methods for the cryopreservation of brain tissue and organoids. Here, we establish a method using methylcellulose, ethylene glycol, DMSO, and Y27632 (termed MEDY) for the cryopreservation of cortical organoids without disrupting the neural cytoarchitecture or functional activity. MEDY can be applied to multiple brain-region-specific organoids, including the dorsal/ventral forebrain, spinal cord, optic vesicle brain, and epilepsy patient-derived brain organoids. Additionally, MEDY enables the cryopreservation of human brain tissue samples, and pathological features are retained after thawing. Transcriptomic analysis shows that MEDY can protect synaptic function and inhibit the endoplasmic reticulum-mediated apoptosis pathway. MEDY will enable the large-scale and reliable storage of diverse neural organoids and living brain tissue and will facilitate wide-ranging research, medical applications, and drug screening.

 

 



#2 Mind

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Posted 21 May 2024 - 06:18 PM

Sounds great. I would like to see this replicated by other cryonics researchers. I am wondering what the feasibility of testing it out in situ with full mammal brains is?


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