• Log in with Facebook Log in with Twitter Log In with Google      Sign In    
  • Create Account
  LongeCity
              Advocacy & Research for Unlimited Lifespans

Photo
- - - - -

Glycine mimics life extension by methionine restriction

glycine methionine methionine restriction

  • Please log in to reply
12 replies to this topic

#1 ta5

  • Guest
  • 952 posts
  • 324
  • Location: 

Posted 20 November 2012 - 01:28 AM


' class='bbc_url' title='External link' rel='nofollow external'>http://www.fasebj.org/cgi/content/meeting_abstract/25/1_MeetingAbstracts/528.2']
Dietary glycine supplementation mimics lifespan extension by dietary methionine restriction in Fisher 344 rats
Brind, Malloy, Augie, et al.

Dietary methionine (Met) restriction (MR) extends lifespan in rodents by 30–40% and inhibits growth. Since glycine is the vehicle for hepatic clearance of excess Met via glycine N-methyltransferase (GNMT), we hypothesized that dietary glycine supplementation (GS) might produce biochemical and endocrine changes similar to MR and also extend lifespan. Seven-week-old male Fisher 344 rats were fed diets containing 0.43% Met/2.3% glycine (control fed; CF) or 0.43% Met/4%, 8% or 12% glycine until natural death. In 8% or 12% GS rats, median lifespan increased from 88 weeks (w) to 113 w, and maximum lifespan increased from 91 w to 119 w v CF. Body growth reduction was less dramatic, and not even significant in the 8% GS group. Dose-dependent reductions in several serum markers were also observed. Long-term (50 w) 12% GS resulted in reductions in mean (±SD) fasting glucose (158 ± 13 v 179 ± 46 mg/dL), insulin (0.7 ± 0.4 v 0.8 ± 0.3 ng/mL), IGF-1 (1082 ± 128 v 1407 ± 142 ng/mL) and triglyceride (113 ± 31 v 221 ± 56 mg/dL) levels compared to CF. Adiponectin, which increases with MR, did not change in GS after 12 w on diet. We propose that more efficient Met clearance via GNMT with GS could be reducing chronic Met toxicity due to rogue methylations from chronic excess methylation capacity or oxidative stress from generation of toxic by-products such as formaldehyde. This project received no outside funding.


→ source (external link)




Related posts here and here.
  • like x 2
  • Informative x 1

#2 Galaxyshock

  • Guest
  • 1,438 posts
  • 178
  • Location:Finland

Posted 20 November 2012 - 02:35 AM

Intredasting
  • dislike x 2
  • Enjoying the show x 1

sponsored ad

  • Advert
Click HERE to rent this advertising spot for SUPPLEMENTS (in thread) to support LongeCity (this will replace the google ad above).

#3 Chupo

  • Guest
  • 321 posts
  • 230
  • Location:United States

Posted 17 March 2013 - 06:23 AM

So, it looks like it's more about the ratio of glycine to methionine. Gelatin, bone broths, and tendons are high in glycine and very low in methionine. According to Cronometer, an ounce of gelatin has 5.3 grams of glycine and 0.2 grams of methionine. I know some here are on methioning restricted diets but do any of you try to increase your glycine intake or supplement glycine?

Edited by Chupo, 17 March 2013 - 06:41 AM.


#4 Sillewater

  • Guest
  • 1,076 posts
  • 280
  • Location:Canada
  • NO

Posted 17 March 2013 - 07:20 AM

none of the mice lived very long.
  • like x 1

#5 Turnbuckle

  • Location:USA
  • NO

Posted 17 March 2013 - 12:03 PM

none of the mice lived very long.


Exactly. I pulled this chart from another study where the longest lived control mice lived to 154 weeks, with a mean of 114 weeks.

http://www.sciencedi...031938497004642

Attached Files


Edited by Turnbuckle, 17 March 2013 - 12:26 PM.

  • like x 1

#6 GABAergic

  • Guest
  • 349 posts
  • -102
  • Location:Maine

Posted 08 January 2019 - 09:12 PM

i found this alarming study though where it claims glycine can cause stroke; https://www.ncbi.nlm...pubmed/25833775 although it was combined with glutamic acid, still, its discomforting nevertheless



#7 brosci

  • Guest
  • 269 posts
  • 31
  • Location:USA

Posted 09 January 2019 - 06:59 PM

What strikes me as interesting is the claim here that:

https://examine.com/...ements/glycine/

http://ergo-log.com/...s-stronger.html

https://chrismasterj...nel-discussion/

Glycine is a conditionally essential amino acid in humans because humans are unable to synthesize enough glycine to satisfy metabolic requirements.[4] The average adult human (70 kg; 30-50 years; sedentary) requires nearly 15 grams of glycine per day to synthesize collagen (12 g/d), non-collagen proteins (1 g/d), and other important compounds such as porphyrins (240 mg/d), purines (206 mg/d), creatine (420 mg/d), glutathione (567 mg/d), and bile salts (60 mg/d).[4] However, glycine synthesis is limited to about 2.5 grams per day, suggesting that humans require about 12 grams of dietary glycine to satisfy daily metabolic requirements.[4]

 

"An important key to understanding these results is the fact that glycine, the most necessary amino acid for collagen synthesis, is highly essential so it must necessarily be incorporated into the diet as a nutritional supplement"

 

"For people who have high rates of collagen turnover and poor recovery of glycine, they could run glycine deficits up to 40-60 grams per day."

If I look on my cronometer report, I usually average around 6g/d while eating what seems like a decent amount of meat and foods that may contain some glycine.  Even if I added 2 tablespoons of gelatin per day, I'd be below the minimum intake. By these metrics... gylcine inadequancy would seemingly be among the most common nutrient deficiencies. Further, it doesn't seem like an inadequate intake leads to an acute disease in the short term, but a lifetime of it seems to accelerate aging and the onset of some chronic disease or overall frailty.

 

https://www.ncbi.nlm...les/PMC5855430/

https://www.ncbi.nlm...les/PMC5350494/

 


Edited by brosci, 09 January 2019 - 07:02 PM.

  • Informative x 3
  • Agree x 1

#8 smithx

  • Guest
  • 1,433 posts
  • 451

Posted 10 January 2019 - 09:38 PM

i found this alarming study though where it claims glycine can cause stroke; https://www.ncbi.nlm...pubmed/25833775 although it was combined with glutamic acid, still, its discomforting nevertheless

 

Good find, but you misinterpreted it. That study found that glycine increased and glutamic acid decreased the risk of stroke.

 

Here's the last paragraph of the discussion in the study:

 

In conclusion, glutamic acid intake was associated with a
decreased risk of mortality from total stroke in women, and
glycine intake was associated with an increased risk of mortality
from ischemic stroke in men without a history of hypertension
.
Because neither animal nor vegetable protein was related to the
risk of stoke mortality, it is tempting to consider the possibility
that the type of amino acid rather than the broad category of
protein (vegetable or animal) has implications for stroke
development. If our findings are true, they would also motivate
clinical studies investigating the role of dietary amino acids in
stroke control. Replication of the results is needed in other
cohort studies

 


Edited by smithx, 10 January 2019 - 09:40 PM.


#9 GABAergic

  • Guest
  • 349 posts
  • -102
  • Location:Maine

Posted 12 January 2019 - 08:48 PM

glycine can increase the risk of stroke. i dont think i misunderstood this part. thats not something to be taken lightly. is there a real good reason for one to take glycine for anything considering over time it can increase risk of stroke?


  • Needs references x 2
  • unsure x 1

#10 smithx

  • Guest
  • 1,433 posts
  • 451

Posted 12 January 2019 - 10:48 PM

According to the study you cited, more glycine in the diet alone increases stroke risk, not combined with glutamic acid.

 

More glutamic acid in the diet alone decreased the risk of stroke.

 

glycine can increase the risk of stroke. i dont think i misunderstood this part. thats not something to be taken lightly. is there a real good reason for one to take glycine for anything considering over time it can increase risk of stroke?

 



#11 Phoebus

  • Guest
  • 851 posts
  • 237
  • Location:Upper Midwest, US

Posted 20 March 2019 - 10:04 PM

 

Epigenetic regulation refers to changes, such as the addition of chemical structures or proteins, which alter the physical structure of the DNA, resulting in genes turning on or off. Unlike mutations, these changes do not affect the DNA sequence itself. If this theory is correct, then genetically reprogramming the cells to an embryonic stem cell-like state would remove any epigenetic changes associated with the mitochondrial DNA. In order to test this theory, the researchers reprogrammed human fibroblast cell lines derived from young and elderly people to an embryonic stem cell-like state. These cells were then turned back into fibroblasts and their mitochondrial respiratory function examined. Incredibly, the age-associated defects had been reversed -- all of the fibroblasts had respiration rates comparable to those of the fetal fibroblast cell line, irrespective of whether they were derived from young or elderly people. This indicates that the aging process in the mitochondrion is controlled by epigenetic regulation, not by mutations.

The researchers then looked for genes that might be controlled epigenetically resulting in these age-associated mitochondrial defects. Two genes that regulate glycine production in mitochondria, CGAT and SHMT2, were found. The researchers showed that by changing the regulation of these genes, they could induce defects or restore mitochondrial function in the fibroblast cell lines. In a compelling finding, the addition of glycine for 10 days to the culture medium of the 97 year old fibroblast cell line restored its respiratory function. This suggests that glycine treatment can reverse the age-associated respiration defects in the elderly human fibroblasts.

https://www.scienced...50526085138.htm

 

Glycine regulating genes were able to restore very old cells to act like young cells again. 


  • Informative x 2

#12 Daniel Cooper

  • Member, Moderator
  • 2,627 posts
  • 629
  • Location:USA

Posted 21 March 2019 - 01:24 PM

Just as an aside, how does one "mimic life extension"?

 

Does it make you feel like you're living longer?

 

 

 


Edited by Daniel Cooper, 21 March 2019 - 01:24 PM.

  • Cheerful x 2

sponsored ad

  • Advert
Click HERE to rent this advertising spot for SUPPLEMENTS (in thread) to support LongeCity (this will replace the google ad above).

#13 Phoebus

  • Guest
  • 851 posts
  • 237
  • Location:Upper Midwest, US

Posted 21 March 2019 - 03:40 PM

Just as an aside, how does one "mimic life extension"?

 

Does it make you feel like you're living longer?

 

I know you are joking but the reason for the use of 'mimic' is that this happened in a petri dish, not in vivo







Also tagged with one or more of these keywords: glycine, methionine, methionine restriction

1 user(s) are reading this topic

0 members, 1 guests, 0 anonymous users