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Resveratrol vs Curcumin? Sirtuins on CD4 and CD8 Human Cells

resveratrol curcumin sirtuins human cells micelle curcumin metacurcumin

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#1 Anthony_Loera

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Posted 21 May 2015 - 05:18 PM


FYI: Hector Valenzuela has asked me to forward any questions you may have on this ongoing study below.

 

Resveratrol vs Curcumin: Is one better than the other? As part of a study currently in process that uses multiple compounds thought to extend life, Curcumin and Resveratrol were compared in immune cells. The following images are taken from the power point slide shown at the American Association of Immunology conference that was held earlier this month.
 
Micronized Resveratrol and MetaCurcumin was provided by RevGenetics for these tests. While I only show Resveratrol and Curcumin here, this is an ongoing study that is also comparing rapamycin, spermidine and nicotinamide mononucleotide (NMN). More data will be provided later as the study gets closer to completion. In the images below we can see the differences in activation of Sirtuins between Resveratrol and Curcumin on immune system cells:
 
 
 
Title: Effects of natural supplementation on human T cell longevity pathways
 
Authors: Nicholas Casarez, Lisa Hom, Sarah Daoudi and Hector Valenzuela
 
Abstract:

Nutraceuticals are natural compounds that contain both nutritional as well as medicinal benefits. Various nutraceuticals (curcumin and resveratrol) obtained from plant extracts have been shown to improve fitness and extend lifespan by various mechanisms. In addition other compounds have also been shown to extend lifespan (rapamycin, spermidine and nicotinamide mononucleotide). Although the end result for these compounds maybe extension of lifespan, comparison of their signaling pathways remains to be determined. The objective of our research was to compare the signaling pathway of these compounds on both normal human T cells and Jurkat cell lines. We used RT-PCR to measure gene expression of NF-Κβ, AMPK, sirtuin 1-7 and autophagy specific genes (ATG5 and MAPILC3B/LC3) for all of these compounds.  In addition, we show a direct correlation between concentrations of treatment and the upregulation of gene expression to the viability of the cells

 

Methods:

Resveratrol, Curcumin and Spermidine were obtained from RevGenetics. The equivalent of 0.05 gm of each compound was dissolved in 10 ml of Ethanol to achieve stock concentrations of 5 x 10-3 gm/mL. Serial dilutions ranging between 10-3, 10-4, 10-5 and 10-6 gm/mL were tested for each compound on our cell cultures. The total volume of the compounds added for each concentration into the experimental cultures represented 0.1% of the final volume. CD4 and CD8 T cells were purified from the PBMC by negative selection, using a C4 and CD8 T cell isolation kit (Miltenyi Biotec) according to the manufacturer's instructions. The T cells were stimulated with CD2/CD3/CD28 antibody-coated beads. The cells (donor’s T cells and/or Jurkat cells) were treated with the compounds and samples taken 24 hours later. Cell samples were taken for telomerase analysis(RT-PCR Millipore kit) and gene expression using RT-PCR (Bio-Rad). Statistical analysis was done using a two tail Student’s t test and significance established with a p value of < 0.05.

 

(GAPDH was used as a housekeeping gene for normalization ie. internal control.)

a184bfed-119c-4291-849a-a70df83e6277.png 3c0314f3-a6ea-464e-93cd-e1d91bf27e74.png

 

Conclusions (so far)...

Curcumin has traditionally been used for centuries by India and China as herbal teas that promote health. Resveratrol is the active ingredient in red wine that is believed to provide cardiovascular and cancer protection. Recent scientific studies have shown that both compounds are anti-inflammatory and may work through similar pathways. Our results, show that both curcumin and resveratrol can modulate sirtuin expression. However, not all extracts versions can stimulate sirtuins and/or telomerase activity to the same degree. Further studies on normal cells should help confirm or change our observations. Nevertheless, we feel that this preliminary study begins to lay the foundation for, not only comparing different forms of curcumin or resveratrol, but also other natural telomerase activators such as spermidine

 

 

For those wondering about the last sentence in the conclusion... yes Cells treated with spermidine increased telomerase activity by 2.3 fold.

 


#2 DeadMeat

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Posted 22 May 2015 - 12:00 AM

Very interesting, some questions:

1) What does micronised resveratrol and MetaCurcumin(curcumin particles with tween 80 surfactant) look like when you dissolve it in ethanol? I assume the resveratrol just dissolves, but is the curcumin still colloidal/micellar or does it form a homogeneous solution?

2) What kind of controls are you using for resveratrol and curcumin? And can you explain why the relative expression of the controls at SIRT3, 4, 5 are so different between the tests with curcumin and resveratrol?

Thanks.



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#3 Anthony_Loera

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Posted 05 June 2015 - 06:07 PM

Very interesting, some questions:

1) What does micronised resveratrol and MetaCurcumin(curcumin particles with tween 80 surfactant) look like when you dissolve it in ethanol? I assume the resveratrol just dissolves, but is the curcumin still colloidal/micellar or does it form a homogeneous solution?

2) What kind of controls are you using for resveratrol and curcumin? And can you explain why the relative expression of the controls at SIRT3, 4, 5 are so different between the tests with curcumin and resveratrol?

Thanks.

 

Per Hector Valenzuela, PhD

 

1) Resveratrol completely dissolves and goes into the solution. Curcumin does present more of a challenge depending on which curcumin we use. Metacurcumin is easier to dissolve since it is already in viscous solution.

 

Now... I did get the answer for question 2...

 

however I was warned that the possibility of "getting scooped" may occur with the information I have been provided, as the project is still active and has not been published yet.

 

Dr V, has asked me to release the full information only to collaborators.

Below is a small part of the answer provided to me:

 

2) We use house-keeping genes to normalize the relative gene expression between the samples. 

 

 

A



#4 Dolph

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Posted 05 June 2015 - 08:35 PM

Do these results allow any practical conclusions?


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#5 Anthony_Loera

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Posted 14 June 2015 - 03:19 AM

Do these results allow any practical conclusions?

 

The preliminary data shows that:

 

Resveratrol (Micronized resveratrol) used in this experiment activated:

 

Sirt1, Sirt2, and Sirt5  (3 Sirtuins) in CD4 Immune White Blood Cells

Sirt4                            (1 Sirtuin) in CD8 Immune White Blood Cells

 

 

While Micelle Curcumin (Metacurcumin) used in this experiment activated:

 

Sirt1, Sirt2, Sirt5, Sirt6, Sirt7 (5 Sirtuins) in CD4 Immune White Blood Cells 

Sirt2, Sirt3                              (2 Sirtuins) in CD8 Immune White Blood Cells

 

If you are a fan of Sirtuins then we can see that the micelle curcumin seems to beat micronized resveratrol in the number of sirtuins activated, although resveratrol could activate Sirt4, which the micelle curcumin did not appear to do.

 

 

While SIRT4 down-regulates insulin secretion in response to amino acids. SIRT4 regulates fatty acid oxidation and mitochondrial gene expression in liver and muscle cells, and is considered a tumor suppressor... but there is little information of resveratrol Sirt4 activation in CD8 immune cells.

 

Regarding Cd4 & CD8 immune cells, it may be best to consider micelle curcumin at this time.

 

A


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#6 pleiotropic

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Posted 31 August 2015 - 04:48 AM

Is there any toxicity studies done for Metacurcumin?







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