27 replies to this topic

[TheFountain]

I remember our discussion a few years ago about Autophagy and Trehalose.

 

Anybody taking it for this and what are the noticeable effects and what should one be aware of when supplementing Trehalose? 

[pleiotropic]

Should be no noticeable effects in humans when taken orally. More than 99.95% converts to glucose during digestion.   Only mice can take it orally.  Rats also convert it to glucose like humans.

 

Human treatment requires IV for it to act as any kind of useful therapy, i.e. autophagy.

 

See this PDF presentation in relation to IV administration in humans: http://phx.corporate...FR5cGU9MQ==&t=1

 

Maybe sublingual is an option?  I don't know if there is any data on that though.

Edited by pleiotropic, 12 November 2015 - 07:53 AM.

[Logic]

The below was found during a search to find a way of getting Trehalose into one's system without it being broken down by Trehalase.
Sadly blocking Threhalase in the gut will cause stomach upsets?

So it looks like the only practical way of taking Trehalose is by injection?

Glycosidase inhibitors: update and perspectives on practical use

http://glycob.oxford.../13/10/93R.full

[Logic]

First Human Use of High Dose IV Trehalose: Safety, Tolerability and Pharmacokinetic Results from the Oculopharyngeal Muscular Dystrophy (OPMD) Therapy Trial (P7.068)

http://www.neurology...pplement/P7.068

 

BioBlast Pharma Announces Preclinical and Clinical Pharmacokinetic Results With Cabaletta, Interim Safety Results From Phase 2/3 HOPEMD Study in OPMD - See more at: http://globenewswire...D-Study-in-OPMD

http://globenewswire...dy-in-OPMD.html

 

[zorba990]

Topical?
http://www.ncbi.nlm....pubmed/23884023

Maybe with DMSO?

"Topical administration of non-steroidal anti-inflammatory drugs (NSAIDs) is generally considered safer than oral administration, although the former can occasionally induce cutaneous irritation. We hypothesized that the cutaneous irritation by topical NSAIDs might be suppressed by trehalose, which has protective effects on biological membranes. Using the three-dimensional cultured human skin model, Living Skin Equivalent-high, we found that cutaneous damage due to NSAIDs was reduced by concomitant use of trehalose and that this effect of trehalose was reinforced by co-lyophilization of NSAIDs with trehalose. The anti-inflammatory effect of co-lyophilized NSAIDs with trehalose was comparable to that seen with NSAIDs alone in a rat model. Our results suggest that co-lyophilization of NSAIDs with trehalose might be a novel procedure that can help prevent NSAIDs-induced skin irritation."

Dmso also causes skin irritation in undiluted form, so maybe this is a win-win dmso+trehalose !

Edited by zorba990, 12 November 2015 - 04:27 PM.

[TheFountain]

So is there any benefit at all of using trehalose as a sugar substitute? 

[Turnbuckle]

We will eventually get a clue from this study--Oral Trehalose Therapy to Reverse Arterial Aging in Middle-Aged and Older Adults, which recently ended but has not yet been published.

 

 

Recent preclinical findings from our lab indicate that drinking water supplemented with trehalose reduces large elastic artery stiffness, restores NO- mediated EDD, reduces arterial oxidative stress, and reverses age-associated declines in autophagy markers in the aorta of old C57/BL6 mice. However, the effect of trehalose supplementation on vascular function in MA/O adults is entirely unknown. The goal of the present application is to translate these preclinical observations to humans. 

 

 

 

Seems to me that taking a large enough dose on an empty stomach would likely overwhelm any enzyme system.

[TheFountain]

We will eventually get a clue from this study--Oral Trehalose Therapy to Reverse Arterial Aging in Middle-Aged and Older Adults, which recently ended but has not yet been published.

 

 

Recent preclinical findings from our lab indicate that drinking water supplemented with trehalose reduces large elastic artery stiffness, restores NO- mediated EDD, reduces arterial oxidative stress, and reverses age-associated declines in autophagy markers in the aorta of old C57/BL6 mice. However, the effect of trehalose supplementation on vascular function in MA/O adults is entirely unknown. The goal of the present application is to translate these preclinical observations to humans. 

 

 

 

Seems to me that taking a large enough dose on an empty stomach would likely overwhelm any enzyme system.

Can you go into more detail on how this would effect us long term?

 

I am fairly sure the discussion we had on trehalose a few years ago was based on a large amount of references too. 

[Mian Ali Ismail]

When will  the results of oral trehaolse results be released ?

 

[adamh]

I tried it a few years ago, started a thread on it. Never noticed any benefit and quit using it but I took it orally. Would sublingual work? I'm not too fond of doing it via dmso because it brings along any contaminants too.

[Mian Ali Ismail]

Has anyone tried it Intraveneously

Has anyone tried it Intraveneously

[Logic]

Has anyone tried it Intraveneously

 

IIRC the only time positive results were seen, was with intravenous dosing.

[Skyguy2005]

Felt nothing at all with trehalose. This is odd because almost every famous supplement (resveratrol, etc.) makes me feel something. Glucosamine, for example, is very distinctive (in a good way), while trehalose... no..., perhaps its a bioavailability thing. 

[normalizing]

how does (distinctevely in a good way) glucosamine make you feel?

[Mian Ali Ismail]

SKYGUY are you taking trehalose Orally ?

[Skyguy2005]

how does (distinctevely in a good way) glucosamine make you feel?

 

Naturally, it's hard to describe. Firm skin, actually I've noticed some pretty extraordinary things with supplements - Ginkgo Biloba turned my wavy hair into Einstein crazy curls and wildness (this was after about 7 months of 5-6x120mg or so a day I was taking for potential nootropic effect).  

 

I rate glucosamine in my top 3. The other 2 would be Ginkgo + Reishi. Ginkgo, Reishi, Resveratrol, Green Tea, Turmeric, Valerian, all feel similar (physically uplifting). Strongest is definitely Ginkgo Biloba, It's actually enough that I'd spoon maybe a gram of raw leaf powder or even less into a pint of water I can instantly tell if it's there. 

 

Glucosamine makes my skin get a really "firm" feeling. I also think it makes my lips slightly redder. Is Ginkgo Biloba nootropic? Shrug. Is Glucosamine joint healthy? Shrug. They just feel really good! 

[Skyguy2005]

SKYGUY are you taking trehalose Orally ?

 

I did yes, orally, although I stopped bothering eventually, due to a strong feeling that is was doing bugger all. 

 

Main reason I brought up glucosamine is because it's related vaguely. 

Edited by Skyguy2005, 22 May 2016 - 08:30 PM.

[normalizing]

skyguy you can actually feel resveratrol? that one is very difficult to actually feel!

[RWhigham]

The oral trehalose trial results have been published http://www.impactagi...ull/100962.html

[normalizing]

very strange we showed again and again its not active orally

[Mian Ali Ismail]

Yes thts wht I thought it isnt absorbed orally.Probably the high dosage bypasses some of the conversion or it could be the trial standards might be low.

[adamh]

If sublingual works then that is an easy way to get benefits. Its a little pricey to use as a sugar substitute, I never add sugar anyway but as a supplement if all you need is a spoonful held in the mouth every day, its cheap.

[Skyguy2005]

skyguy you can actually feel resveratrol? that one is very difficult to actually feel!

 

Yes, I could feel amost any herbal type thing (Ginkgo Biloba, Resveratrol, Quercetin, Curcumin, Glucosamine, Green Tea, Ginseng, Valerian Root etc.) 

 

I could probably recognise Ginkgo Biloba extract by sniffing the bottle (I have something of a Ginkgo obsession). Maaybe Red Reishi too. Broadly speaking all the above things I listed have a similar feeling, Glucosamine is a bit different (makes sense). 

Edited by Skyguy2005, 31 May 2016 - 08:18 PM.

[Logic]

Novel autophagy inducers lentztrehaloses A, B and C

Trehalose has widespread use as a sweetener, humectant and stabilizer, and is now attracting attention as a promising candidate for the treatment of neurodegenerative diseases as it is an autophagy inducer and chemical chaperone. However, the bioavailability of trehalose is low because it is digested by the hydrolyzing enzyme trehalase, expressed in the intestine and kidney. Enzyme-stable analogs of trehalose would potentially solve this problem. We have previously reported an enzyme-stable analog of trehalose, lentztrehalose, and herein report two new analogs. The original lentztrehalose has been renamed lentztrehalose A and the analogs named lentztrehaloses B and C. Lentztrehalose B is a di-dehydroxylated analog and lentztrehalose C is a cyclized analog of lentztrehalose A. All the lentztrehaloses are only minimally hydrolyzed by mammalian trehalase. The production of the lentztrehaloses is high in rather dry conditions and low in wet conditions. Lentztrehalose B shows a moderate antioxidative activity. These facts suggest that the lentztrehaloses are produced as humectants or protectants for the producer microorganism under severe environmental conditions. All the lentztrehaloses induce autophagy in human cancer cells at a comparable level to trehalose. Considering the enzyme-stability, these lentztrehaloses can be regarded as promising new drug candidates for the treatment of neurodegenerative diseases and other autophagy-related diseases, such as diabetes, arteriosclerosis, cancer and heart disease.

 

http://www.nature.co.../ja201523a.html

http://www.longecity...ism-discovered/

[normalizing]

ok so lets keep an eye on those when they become available on the market cheers

[normalizing]

uhm actually trehalose is bad news; http://www.post-gaze...es/201801030272 it encourages the spread of two superbugs according to this article :/

[Daniel Cooper]

That article is jumping to a hell of a conclusion.  If they are looking at superbugs in U.S. hospitals, they may move proved that some strains like to eat trehalos - I don't doubt that you will find many different strains that have a fondness for one sugar over another, but finding that a bug likes to consume trehalose in vitro is far from proving that trehalose has any role whatsoever in spreading superbugs in U.S. hospitals.  Trehalose is very rare in the U.S. food supply.  I know, I've looked for products sweetened with trehalose and basically you just can't find them in this country.  A vanishingly small percentage of patients will be consuming any amount of trehalose.  So whatever is fueling superbugs in U.S. hospitals, it isn't trehalose.  

 

Now, trehalose is much more commonly used as a sweetener in Japan and to a somewhat lesser extent in Australia.   Are those countries seeing a dramatic rise in these strains of superbugs?  Not that I've seen reported. 

 

If you have these strains of bugs in your gut, you have a problem no matter what sweetener you're consuming. 

 

BTW - trehalose is almost entirely broken down in the gut as the studies in this thread amply demonstrate.  IV trehalose will obviously achieve significant blood plasma concentrations, but most of us aren't equipped to administer an IV to ourselves.  What we need is liposomal trehalose to get it though the gut intact.   I'm talking with a manufacturer of liposomal supplements.  My goal is to convince them that they should carry liposomal trehalose as a standard product. Barring that, they have offered that they will make it to order with a 4L minimum.  That's much less attractive to me as I don't want the hassle of managing a group buy, but I'm interested in this product enough for my own use that I may go that route as a last resort.

 

 

 

[normalizing]

yeah, the way i see it, the only sure proven way to create superbugs is basically overuse of antibiotics and thats it! im really not worried about any of the other things like trehalose honestly. i just saw this article and find it amusing and decided to share