10 replies to this topic

[stefan_001]

A thread focused solely on discussion about new or recent research reports and results into and/or achieved with NR.

Reason: threads opened around single research reports do not attract enough discussion and therefore quickly dissapear from the radar.

Request: Please share your NR research findings and express your views about the reports

 

Here some reports published in the last couple months:

Nicotinamide riboside, a form of vitamin B3, protects against excitotoxicity-induced axonal degeneration

http://www.fasebj.or...0221RR.abstract

"Taken together, our findings demonstrate that NR is a better neuroprotective agent than NAD+ in excitotoxicity-induced AxD and that axonal protection involves defending intracellular NAD+ homeostasis."

 

Nicotinamide Riboside and the Aortic Response to Angiotensin II Infusion in Mice

http://ir.lib.uwo.ca/etd/4788/

"I demonstrate that orally administered NR can protect the aorta from damage imposed by Ang II."

 

Hepatic NAD~+ deficiency as a therapeutic target for NAFLD in aging

http://www.cnki.com....BS201610142.htm

"Orally administration of nicotinamide riboside,a natural NAD+precursor,completely corrected these NAFLD phenotypes induced by NAD+deficiency alone or HFD,whereas adenovirusmediated SIRT1 overexpression only partially rescued these phenotypes."

 

The therapeutic role of NAD+ modulation in fatty liver disease

https://infoscience....h/record/229863

In this study we evaluated the potential of Nicotinamide Riboside (NR) as a therapeutic agent against the development of NAFLD. First, we observed a positive correlation between gene sets for β-oxidation and genes involved in NAD+ biosynthesis in two distinct data sets of healthy human liver samples supporting the function of NAD+ as a coenzyme in key metabolic processes. Then we showed that NAD+ repletion using NR, an NAD+ booster, attenuated mitochondrial dysfunction and prevented and reversed fatty liver development in a several mouse models of NAFLD.

 

[stefan_001]

Positive research around liver health keeps coming and coming, I like that as a healthy liver has a whole body positive impact.

[Michael]

Oh, please let's not do that. That's how the old [Curated] thread became an unmanageable clusterfuck with ten people talking about four different subjects at a time, people resurrecting discussions from 5 pages back, being unable to find anything with or without the search, etc. Individual theme or study threads make for clean and navigable discussion.

[stefan_001]

Oh, please let's not do that. That's how the old [Curated] thread became an unmanageable clusterfuck with ten people talking about four different subjects at a time, people resurrecting discussions from 5 pages back, being unable to find anything with or without the search, etc. Individual theme or study threads make for clean and navigable discussion.

 

@Michael please look through the first page of the opened threads, do you see anything sticking around research into NR? They will vanish, even harder to find then before because with the old [curated] thread you at least knew where to look. For example you opened this one (well about NMN but same issue):

http://www.longecity...mito-regulator/

 

with cut out context that took you time. But zero reactions so probably in a week it will have dissapeared from sight forever. You have a better suggestion? I miss the science focus on NR. Perhaps there is a way in the middle where the research outcomes are segmented:

- research outcomes wrt neuroprotection

- research outcomes wrt heart

- research outcomes wrt liver and other organs

- etc

 

but also for this I am afraid there are not enough contributors

Edited by stefan_001, 21 September 2017 - 09:06 PM.

[stefan_001]

Fresh NMN study, almost NR :-)

 

NAD replenishment with nicotinamide mononucleotide protects blood–brain barrier integrity and attenuates delayed tissue plasminogen activator-induced haemorrhagic transformation after cerebral ischaemia

http://onlinelibrary.../bph.13979/full

 

BBB Blood-Brain Barrier permeability is often cited in aging and Alzheimer's Disease

Edited by stefan_001, 21 September 2017 - 09:37 PM.

[stefan_001]

The role of nicotinamide riboside kinases in mammalian NAD⁺ metabolism

https://infoscience....h/record/229935

Using NRK1 liver-specific KO mice, we demonstrate that NRK1 is required to maintain hepatic NAD+ levels and mitochondrial function upon high-fat feeding........Moreover, it has unveiled a critical role of NRK1 for the metabolism of diverse forms of Vitamin B3 and highlights how circulating NR is key to preserve metabolic health upon dietary challenges.

 

I have asked a free copy, anybody already access to this report? sounds like there is good info on the NR metabolism in there which is heavily debated in this forum

Edited by stefan_001, 21 September 2017 - 09:52 PM.

[stefan_001]

From the earlier post #1 chinese study, an important part, this is done in Wild Type mice:

we  further  investigated whether  NR  can  prevent  diet induced  NAFLD in  WT  mice.  NR  treatment  for  4  weeks successfully increased NAD level in liver tissues in HFD fed mice (Supplemental figure 9), and potently  blocked  the  HFD induced  increases of  body  weight  (Fig. 7A) and reduced  the marked  increases  of  hepatic  triglyceride  and  cholesterol  levels  (Fig. 7B).  NR  treatment also reduced liver weight and the ratio of liver weight to body weight (Fig. 7C). Oil red O staining confirmed the decline of hepatic lipid content by NR (Fig. 7D). In addition, SMA staining showed NR treatment significantly lowered the hepatic fibrosis (Fig. 7E). The enhancements of  TNF-and  IL-1 production  in  liver  tissue  were  also  treated  by  NR  treatment (Fig. 7F). These results demonstrate that NR treatment prevents diet induced NAFLD pathogens in WT mice.

 

Other interesting comments:

- By contrast,the expression of NAMPT was significantly lower in aged liver, implying the NAD+ salvage biosynthesis   is   compromised   in   aged   human   liver.

- In our experiments, NAD+ levels not only declines with aging, but also decreases under HFD condition.

Edited by stefan_001, 22 September 2017 - 08:58 AM.

[Michael]

From the earlier post #1 chinese study, an important part, this is done in Wild Type mice:

we  further  investigated whether  NR  can  prevent  diet induced  NAFLD in  WT  mice.  NR  treatment  for  4  weeks successfully increased NAD level in liver tissues in HFD fed mice (Supplemental figure 9), and potently  blocked  the  HFD induced  increases of  body  weight  (Fig. 7A) and reduced  the marked  increases  of  hepatic  triglyceride  and  cholesterol  levels  (Fig. 7B).  NR  treatment also reduced liver weight ... lowered the hepatic fibrosis ... [and] enhancements of  TNF-α and  IL-1-β  production  in  liver  tissue  were  also  treated  by  NR  treatment (Fig. 7F). These results demonstrate that NR treatment prevents diet induced NAFLD pathogens in WT mice.

 

I'm guessing that the reason you're highlighting this is because you think that in describing these as "WT mice," they're saying that they are mice free of the NNT mutation that confounds all (?) the megadose animal NR research. Unfortunately, this too was in NNT-mutation-bearing C57BL/6J mice: "Male C57BL/6J mice were purchased from Sino-British SIPPR/BK Lab Animal Ltd. (Shanghai, China)." Rightly or wrongly, C57BL/6J mice are considered wild-type, both because the mutation was only discovered relatively recently, and because the mutation is not grossly disabling in the way that engineered mutant mice usually are.

[stefan_001]

From the earlier post #1 chinese study, an important part, this is done in Wild Type mice:
we further investigated whether NR can prevent diet induced NAFLD in WT mice. NR treatment for 4 weeks successfully increased NAD level in liver tissues in HFD fed mice (Supplemental figure 9), and potently blocked the HFD induced increases of body weight (Fig. 7A) and reduced the marked increases of hepatic triglyceride and cholesterol levels (Fig. 7B). NR treatment also reduced liver weight ... lowered the hepatic fibrosis ... [and] enhancements of TNF-α and IL-1-β production in liver tissue were also treated by NR treatment (Fig. 7F). These results demonstrate that NR treatment prevents diet induced NAFLD pathogens in WT mice.

I'm guessing that the reason you're highlighting this is because you think that in describing these as "WT mice," they're saying that they are mice free of the NNT mutation that confounds all (?) the megadose animal NR research. Unfortunately, this too was in NNT-mutation-bearing C57BL/6J mice: "Male C57BL/6J mice were purchased from Sino-British SIPPR/BK Lab Animal Ltd. (Shanghai, China)." Rightly or wrongly, C57BL/6J mice are considered wild-type, both because the mutation was only discovered relatively recently, and because the mutation is not grossly disabling in the way that engineered mutant mice usually are.

Indeed and thats unfortunate. Well noted. I find it somewhat puzzling that research wpuld leave such possible influence on there conclusions. Perhaps there is simply not a better model mouse available.

Edited by stefan_001, 22 September 2017 - 04:26 PM.

[Nate-2004]

Oh, please let's not do that. That's how the old [Curated] thread became an unmanageable clusterfuck with ten people talking about four different subjects at a time, people resurrecting discussions from 5 pages back, being unable to find anything with or without the search, etc. Individual theme or study threads make for clean and navigable discussion.

 

Off topic I know, but the reason for that is this old fashioned message board format. There are much newer ways to organize threads in such a way that they do not become unmanageable and impossible to keep up with. Reddit and Disqus have much better organization. Not sure where else to talk about this but I think we should all move to Reddit or something else.

Edited by Nate-2004, 06 October 2017 - 02:32 AM.

[Heisok]

Oh, please let's not do that. That's how the old [Curated] thread became an unmanageable clusterfuck with ten people talking about four different subjects at a time, people resurrecting discussions from 5 pages back, being unable to find anything with or without the search, etc. Individual theme or study threads make for clean and navigable discussion.

 

Search

 

caliban thanks for the explanation. Maybe I am the exception, but I like the straight chronologic order of posts within a topic.

 

One has to use a multi step process to search specific threads.

 

Example:

 

1.I remember something about hypertrophy in the manipulating mitochondrial dynamics thread.

2. I go to the first page of the thread.

3. I cut the URL address from the browser, which in this case is: http://www.longecity...drial-dynamics/

4. In the Google search, I type hypertrophy site: and then append the address. hypertrophy site:http://www.longecity...drial-dynamics/

5. hypertrophy site:http://www.longecity...drial-dynamics/

6. Search results might say that some results were omitted, but just click repeat with those results.

 

It is a pain, but some topics get huge.