LONGECITY

I decided to try my hand at a protocol for this after being inspired by Turnbuckle‘s thread about c60. He said that c60 isn’t good for younger people and specifically mentioned that his protocol isn’t for people under 60, I think that there‘s enough under 60 users here that we need a protocol too.

3-4 Days a Week: Stem Cell/Motochondria Maintenance

1. Niacin 2g

2. D-Ribose 5g

https://www.lifeexte...ifespan/Page-01

I put them separately as both Niagen ingredients get separated in the body anyway and according to Turnbuckle it’s just as effective.

3. Icaarin 500 mg-1g

https://www.ncbi.nlm...les/PMC5789743/

4. Taurine 5g
https://www.ncbi.nlm...les/PMC3423436/

5. TUDCA 500mg-2g

https://www.ncbi.nlm...ubmed/25483094/

2 Days a Week-Senolytics

(protocol courtesy of Turnbuckle but I replaced a vitamin B3 derivative with Niacin.)


1. Apigenin 100mg

2. Resveratrol 200mg

3. Niacin 2g

Let me know what you think and if we can improve this protocol.

+ Ubiquinol?
+ BioPQQ?
+ l-threonine?
Edited by Kentavr, 21 January 2019 - 09:21 PM.

You should look into Alga AFA (Aphanizomenon flos-aquae) it's the strongest stimulant of the stem cells productions, also inhibits the development and formation of tumors. the pigments from its composition are stimulating the production of adult stem cells and the polisacharides from it determines the migration of the cells there where it is needed for the regeneration of the organism. Also make sure your source is not contaminated (so not cheapest you can find usually).

 

https://www.ncbi.nlm...ubmed/20037479 

 

https://juniperpubli...S.ID.555564.pdf

 

not related but worth the post, lol https://www.research...e_immune_system

 

there are more studies floating around, maybe better than the ones I quickly pasted.

 

This is also on my list for a neurogenesis stack, and in that regard, thank you for the link on Taurine. I was already taking Taurine for various reasons but had no idea about what it said in that link, really great to know and it makes sense more now. Also adding it to the neurogenesis stack which will be ready some day. That's another topic, so don't let me hijack the thread.

I have a question, for example, how does the body know/how can we influence where the stem cells are needed more? For example, if say you had candida in your ear and you didn't know and the little bugger kinda started eating tissue and one day you find it was very close to the eardrum already chopping, while my hear is still good with a little bit of tinnitus from years ago (unrelated possibly), if I were to hop on a stack like yours for stem cell maint/production/stimulation, I could still not know for sure if the stem cells would help repair the tissues inside my ear or if it would go towards some minuscule tumour and therefore making it become a big boy which otherwise it wouldn't even survive. Does that make sense? I am just asking because I don't know.

Edited by Bubbles, 21 January 2019 - 10:32 PM.

+ Ubiquinol?
+ BioPQQ?
+ l-threonine?

I would add threonine to the protocol at 5-10 g. Glycine as an alternative makes sense too in my opinion. I read too many questionable reviews about Ubiquinol’s and PQQ’s effectiveness to add them.

You should look into Alga AFA (Aphanizomenon flos-aquae) it's the strongest stimulant of the stem cells productions, also inhibits the development and formation of tumors. the pigments from its composition are stimulating the production of adult stem cells and the polisacharides from it determines the migration of the cells there where it is needed for the regeneration of the organism. Also make sure your source is not contaminated (so not cheapest you can find usually).

https://www.ncbi.nlm...ubmed/20037479

https://juniperpubli...S.ID.555564.pdf

not related but worth the post, lol https://www.research...e_immune_system

there are more studies floating around, maybe better than the ones I quickly pasted.

This is also on my list for a neurogenesis stack, and in that regard, thank you for the link on Taurine. I was already taking Taurine for various reasons but had no idea about what it said in that link, really great to know and it makes sense more now. Also adding it to the neurogenesis stack which will be ready some day. That's another topic, so don't let me hijack the thread.

I have a question, for example, how does the body know/how can we influence where the stem cells are needed more? For example, if say you had candida in your ear and you didn't know and the little bugger kinda started eating tissue and one day you find it was very close to the eardrum already chopping, while my hear is still good with a little bit of tinnitus from years ago (unrelated possibly), if I were to hop on a stack like yours for stem cell maint/production/stimulation, I could still not know for sure if the stem cells would help repair the tissues inside my ear or if it would go towards some minuscule tumour and therefore making it become a big boy which otherwise it wouldn't even survive. Does that make sense? I am just asking because I don't know.

Thank you for the links! I’m reading now and I’m thinking that it would make sense to add it. I’m glad that you found the Taurine link useful, I think that it’s a very underrated supplement and crucial for stem cell activation. It also helps with NAD+ absorption. I’m sorry that you have had hearing problems, my understanding is that the body knows on its own to “send help” to areas that it sees as problematic and in need of help in the body. So, if you have a problem in an area, then the body will send help to that area, such as in this case stem cells. I’m not an expert on that part of the body’s many complex mechanisms, but I’m sure based on what I’ve learned that this is how the body seems to operate.

Blueberry Extract seems to be particularly effective at stimulating stem cell growth, and there's considerable synergy when Carnosine, D3 and/or Green Tea Extract are included.

 

https://www.lifeexte...12/awsi/Page-01

 

Melatonin, Resveratrol and low dose lithium have also been shown to increase stem cells.

 

Blueberry Extract seems to be particularly effective at stimulating stem cell growth, and there's considerable synergy when Carnosine, D3 and/or Green Tea Extract are included.

https://www.lifeexte...12/awsi/Page-01

Melatonin, Resveratrol and low dose lithium have also been shown to increase stem cells.

That’s a great find. I think that adding this might help a lot. Specifically Carnosine, Resveratrol and blueberry extract.

Am researching and building and already experimenting my way towards neurogenesis (NGF  & BDNF) and  I realize that there are quite many supplements in common with the ones from the Stem Cells protocol. Which makes me think I may be able to do both actually; they already do happen to some extent already in everybody you know of course, but I'm referring now to the proper protocol we are diving into. 

Am researching and building and already experimenting my way towards neurogenesis (NGF & BDNF) and I realize that there are quite many supplements in common with the ones from the Stem Cells protocol. Which makes me think I may be able to do both actually; they already do happen to some extent already in everybody you know of course, but I'm referring now to the proper protocol we are diving into.

Which protocol are you referring to and which supplements are you recommending?

I decided to try my hand at a protocol for this after being inspired by Turnbuckle‘s thread about c60. He said that c60 isn’t good for younger people and specifically mentioned that his protocol isn’t for people under 60, I think that there‘s enough under 60 users here that we need a protocol too.

3-4 Days a Week: Stem Cell/Motochondria Maintenance

1. Niacin 2g

2. D-Ribose 5g

https://www.lifeexte...ifespan/Page-01

I put them separately as both Niagen ingredients get separated in the body anyway and according to Turnbuckle it’s just as effective.

3. Icaarin 500 mg-1g

https://www.ncbi.nlm...les/PMC5789743/

4. Taurine 5g
https://www.ncbi.nlm...les/PMC3423436/

5. TUDCA 500mg-2g

https://www.ncbi.nlm...ubmed/25483094/

2 Days a Week-Senolytics

(protocol courtesy of Turnbuckle but I replaced a vitamin B3 derivative with Niacin.)


1. Apigenin 100mg

2. Resveratrol 200mg

3. Niacin 2g

Let me know what you think and if we can improve this protocol.

NeilR, how is your experiment going? What drugs did you choose?
Edited by Kentavr, 23 September 2019 - 03:13 PM.

+ Ubiquinol?
+ BioPQQ?
+ l-threonine?

I actually added Threonine 2 g to the first part of the protocol recently. Also every 5 months I will stop to take Endoluten and Epitslon

NeilR, how is your experiment going? What drugs did you choose?

So far still doing this with Threonine added. I also added Alpha Lipoic acid and daily Hyaluronic Acid orally 1 g. So far no changes, though I’m told that I look younger than my age, but I was typically told this even before I started anti-aging. I haven’t yet done any testing to measure my telomeres and epigenetic age since starting this protocol.


Do you have any ideas on how to improve this?

NeilR, how is your experiment going? What drugs did you choose?

In an older post in Turnbuckle’s topic, you said to combine Endoluten with Vladonix. Why is that better than Endoluten by itself? Also would Везуген help stem cells?

In an older post in Turnbuckle’s topic, you said to combine Endoluten with Vladonix. Why is that better than Endoluten by itself? Also would Везуген help stem cells?

Yes, I talked about 2 years ago with the company's management at the exhibition, as well as with the company's specialists in their official office.

The recommendations for taking Endoluten are as follows:

1. The best time of admission: at 10:00 (established experimentally, according to customer reviews). If Endoluten is taken at 10:00, then this drug acts most potently.

2. The best way to use is to take 1/3 capsule per day at 10:00. When asked why 1/3 of the capsule, it was reported that in this way Endoluten peptides are used most effectively.

The reason why this method is more effective: a small number of cells in the pineal gland (in humans, it is the size of a rice grain). The use of 1/3 capsule per day allows you to use the drug most rationally.

When I asked why the company cannot release a drug with a peptide concentration 3 times lower in the capsule, they said that they would not do this, since all the checks would have to be re-run, which was not beneficial.

3. The peptide is more advantageous to take on an empty stomach, but the benefit is very small: when taken with food, the peptide will be destroyed only 7% of the total amount of the main peptide, which is not critical (calculated data). So it’s a little more profitable to take the peptide on an empty stomach.

4. Any peptides begin to work better when combined with any peptides of the circulatory system (the function of the circulatory system is enhanced, and the peptides reach the desired organ faster and more efficiently).
Edited by Kentavr, 25 September 2019 - 04:07 PM.

Now for the drug ВЕЗУГЕН:

1. ВЕЗУГЕН is a synthetic peptide that works in the vascular system.

People who believe that the largest organ of our body is the skin are very mistaken. The largest organ of our body is the vascular endothelium (!) It has a very large inner surface, and this internal organ forms a large number of cells.

Since the vascular endothelium consists of a large number of cells, for its effective stimulation it is necessary to take at least 2 capsules of ВЕЗУГЕН daily.

Reception time - morning (possible at 10:00). In no case should you take peptides in the evening (!) This is dangerous! Peptides in the evening can greatly increase blood pressure.

In the evening, you can only take Vladonix (and it is necessary, it is best at 19:00, this time is more effective), but it is an exception case.
Edited by Kentavr, 25 September 2019 - 04:32 PM.

My personal experience with ВЕЗУГЕН:

1. After taking the drug, 2 capsules per day for 30 days, my skin turned pink and they began to tell me that I looked younger (at that time I was 35 years old).

2. At the first intake, there may be colic in the abdomen (one of the effects of the drug, since it has a powerful effect on cells associated with vascular endothelium - keep this information in mind).

A repeated course of taking the drug after 4 months caused me severe colic in the abdomen. I took a break for several days and reduced my intake to 1 capsule per day.

VESUGEN and stem cells:

This peptide proved to be a powerful stimulator of vascular endothelial stem cells - 3 (!) Times in vitro (I consulted with the company's top management, and I was informed that it also increases the number of bone marrow stem cells). This may help to understand why my skin began to look younger and fresher.

Link to the article in the journal:

Pay attention to the chart.
H-Lys-Glu-Asp-OH (ВЕЗУГЕН)

http://gerontology.esrae.ru/2-15

Yes, I talked about 2 years ago with the company's management at the exhibition, as well as with the company's specialists in their official office.

The recommendations for taking Endoluten are as follows:

1. The best time of admission: at 10:00 (established experimentally, according to customer reviews). If Endoluten is taken at 10:00, then this drug acts most potently.

2. The best way to use is to take 1/3 capsule per day at 10:00. When asked why 1/3 of the capsule, it was reported that in this way Endoluten peptides are used most effectively.

The reason why this method is more effective: a small number of cells in the pineal gland (in humans, it is the size of a rice grain). The use of 1/3 capsule per day allows you to use the drug most rationally.

When I asked why the company cannot release a drug with a peptide concentration 3 times lower in the capsule, they said that they would not do this, since all the checks would have to be re-run, which was not beneficial.

3. The peptide is more advantageous to take on an empty stomach, but the benefit is very small: when taken with food, the peptide will be destroyed only 7% of the total amount of the main peptide, which is not critical (calculated data). So it’s a little more profitable to take the peptide on an empty stomach.

4. Any peptides begin to work better when combined with any peptides of the circulatory system (the function of the circulatory system is enhanced, and the peptides reach the desired organ faster and more efficiently).

Thank you for such a thorough and important explanation! Do you think that the best thing to do is to take 1/3 of a pill per day until all 20 pills are done and then take a break for a few months as the package recommends?

VESUGEN and stem cells:

This peptide proved to be a powerful stimulator of vascular endothelial stem cells - 3 (!) Times in vitro (I consulted with the company's top management, and I was informed that it also increases the number of bone marrow stem cells). This may help to understand why my skin began to look younger and fresher.

Sounds as if VESUGEN is very effective at making you look younger and at improving stem cell production in the skin. What is your current dosage?

Link to the article in the journal:

Pay attention to the chart.
H-Lys-Glu-Asp-OH (ВЕЗУГЕН)

http://gerontology.esrae.ru/2-15

Kentavr, what’s your Endoluten schedule? Do you take every three days with no break for months?

You should look into Alga AFA (Aphanizomenon flos-aquae) it's the strongest stimulant of the stem cells productions, also inhibits the development and formation of tumors. the pigments from its composition are stimulating the production of adult stem cells and the polisacharides from it determines the migration of the cells there where it is needed for the regeneration of the organism. Also make sure your source is not contaminated (so not cheapest you can find usually).

https://www.ncbi.nlm...ubmed/20037479

https://juniperpubli...S.ID.555564.pdf

not related but worth the post, lol https://www.research...e_immune_system

there are more studies floating around, maybe better than the ones I quickly pasted.

This is also on my list for a neurogenesis stack, and in that regard, thank you for the link on Taurine. I was already taking Taurine for various reasons but had no idea about what it said in that link, really great to know and it makes sense more now. Also adding it to the neurogenesis stack which will be ready some day. That's another topic, so don't let me hijack the thread.

I have a question, for example, how does the body know/how can we influence where the stem cells are needed more? For example, if say you had candida in your ear and you didn't know and the little bugger kinda started eating tissue and one day you find it was very close to the eardrum already chopping, while my hear is still good with a little bit of tinnitus from years ago (unrelated possibly), if I were to hop on a stack like yours for stem cell maint/production/stimulation, I could still not know for sure if the stem cells would help repair the tissues inside my ear or if it would go towards some minuscule tumour and therefore making it become a big boy which otherwise it wouldn't even survive. Does that make sense? I am just asking because I don't know.

Great post, thanks for this. Strange that I've never heard of StemEnhance and yet it has quite a few positive animal studies in pubmed. For example this one in mice with liver cirrhosis:

https://www.ncbi.nlm...ubmed/25857836/

This study is the first to evaluate the beneficial effect of StemEnhance administration in a thioacetamide-induced mouse model of liver fibrosis. StemEnhance augmented the number of peripheral CD34-positive cells, reduced hepatic fibrosis, improved histopathological changes, and induced endogenous liver proliferation. In addition, VEGF expression was up-regulated, while TNF-α expression was down-regulated in thioacetamide-induced fibrotic livers after StemEnhance intake

This probably answers your question as to how do stem cells know where to go. By increasing the number in circulation you'd increase the chance of them dealing with any current problem.

The only question in my mind is would doing this regularly reduce your store of stem cells long term. So might be an idea to cycle this with Turnbuckle's protocol. I might also add this supplement to my statin-epitalon-sartan cycle so I can increase the stem cell numbers even further before I let them differentiate normally.
Edited by QuestforLife, 12 October 2019 - 07:01 AM.

Has anyone gone through with any of these protocols for extended periods of time?  If so, any noticeable benefits or side effects?

 

Also, I don't see any mention of stearic acid, which was a key component of the C60 protocol in order to promote self-renewal over differentiation.  It seems to me that the same idea should apply here, even if we use blueberry/D3/Vesugen/icariin/AFA/TUDCA/etc. instead of C60 for the proliferation aspect.

Also, I don't see any mention of stearic acid, which was a key component of the C60 protocol in order to promote self-renewal over differentiation.  It seems to me that the same idea should apply here, even if we use blueberry/D3/Vesugen/icariin/AFA/TUDCA/etc. instead of C60 for the proliferation aspect.

Or sulforaphane, also said to promote mitochondrial fusion and, in addition, to be able to cross the blood-brain barrier.

 

Figure 3 of this paper suggests that peak concentration in plasma is reached 2 hours after ingestion.

https://www.ncbi.nlm...les/PMC7070302/

 

The authors state "Our intervention peaked slightly later (~2hrs), than that of Fahey (~1 hr), likely due to our use of a capsule rather than liquid..."

Edited by JamesPaul, 27 January 2021 - 03:39 AM.

Thank you for the links! I’m reading now and I’m thinking that it would make sense to add it. I’m glad that you found the Taurine link useful, I think that it’s a very underrated supplement and crucial for stem cell activation. It also helps with NAD+ absorption. I’m sorry that you have had hearing problems, my understanding is that the body knows on its own to “send help” to areas that it sees as problematic and in need of help in the body. So, if you have a problem in an area, then the body will send help to that area, such as in this case stem cells. I’m not an expert on that part of the body’s many complex mechanisms, but I’m sure based on what I’ve learned that this is how the body seems to operate.

 

NeilR,

 

How are you doing? What are the results?

I would re-evaluate the Taurine.

Taurine supplementation has been shown to downregulates GABA. This can lead to excitatory issues in the brain.

"The brains of mice chronically treated with taurine have decreased levels of GABA(A)β subunits and increased expression of GAD and GABA, which contributes to hyperexcitability. This down regulation of GABA(A)receptor subunit expression and function may be due to a sustained interaction of taurine with GABA(A)receptors."

:text=The%20brains%20of%20mice%20chronically,with%20GABA(A)receptors' class='bbc_url' title='External link' rel='nofollow external'>https://pubmed.ncbi....ABA(A)receptors.

Great post, thanks for this. Strange that I've never heard of StemEnhance and yet it has quite a few positive animal studies in pubmed. For example this one in mice with liver cirrhosis:

https://www.ncbi.nlm...ubmed/25857836/

This probably answers your question as to how do stem cells know where to go. By increasing the number in circulation you'd increase the chance of them dealing with any current problem.

The only question in my mind is would doing this regularly reduce your store of stem cells long term. So might be an idea to cycle this with Turnbuckle's protocol. I might also add this supplement to my statin-epitalon-sartan cycle so I can increase the stem cell numbers even further before I let them differentiate normally.

Did you ever try the StemEnhance supplement? 

I've been thinking about it recently, and I'd love to know how it worked for you