Reverse-expression of aging-associated mol...
alc
30 Dec 2016
This is a study that hint at what path are taking groups like George Church's, Calico, etc.
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Abstract
Molecular changes during aging have been studied to understand the mechanism of aging progress.
Herein, changes in miRNA expression in the whole blood of mice were studied to systemically
reverse aging and propose as non-invasive biomarkers. Through next generation sequencing analysis,
we selected 27 differentially expressed miRNAs during aging. The most recognized function involved
was liver steatosis, a type of non-alcoholic fatty liver disease (NAFLD). Among 27 miRNAs,
six were predicted to be involved in NAFLD, miR-16-5p, miR-17-5p, miR-21a-5p, miR-30c-5p, miR-103-3p,
and miR-130a-3p; alterations in their blood and liver levels were confirmed by quantitative real-time
polymerase chain reaction. The expression of the genes associated in the network of these miRNAs,
Bcl2, Ppara, E2f1, E2f2, Akt, Ccnd1, and Smad2/3, was also altered in the liver of aged mice.
Following transfection of these miRNAs into 18-month-old mice, levels of miR-21a-5p, miR-103-3p,
and miR-30c-5p increased, and their related genes exhibited a reversed expression in the liver.
Expression of Mre11a, p16INK4a, and Mtor, reported to be aging-associated molecules, was
also reversed in the livers of miRNA-transfected mice. These miRNAs could be non-invasive
biomarkers for aging, and might induce a reverse-regulation of aging-associated pathways.
This study provides preliminary data on reverse-aging, which could be applied further
for treatments of adult diseases.
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