LONGECITY

I have to say I don't really understand the new protocol at all.  I can't figure out if people here are doing it correctly or not.  Turnbuckle - who has been a huge inspiration and contributor to this thread - does not really explain anything in the LinkedIn post, unlike his previous protocols.  The graph is unclear to me.  It seems like the 71 days before lactate were useless, but others here seem to have a different interpretation.  I had no problem whatsoever with any of the previous protocols, as they were thoroughly explained.

I'd like to try this protocol if I can be confident in doing it correctly.  Any further clarification by Turnbuckle, or anyone here, would be very helpful.

Thank you!

Agree. The way I understand the graph is that it is a diary in that it is a chronological progression.

 

blue circles: fission

orange circles: fusion

green circles: no treatment days

blue circles with black circles around them: fission with a deviation

black circles: not sure

red circles: lactate with or without AAKG

red circles with black circles around them: like red but with addition of lactate

I've blown up and annotated the Inner Membrane Ratchet diagram, there are a couple of places where I wasn't sure of the content which I have bracketed with ? marks. I'm keen to see if the ratchet works as it could give extra capacity above the baseline.  I'll try that in a couple of months as I'm currently working on a block of 8-10 cycles of the earlier Mito protocol.

ConsumerLab only shows LE 100mg CoQ10 with PQQ. I'm pretty sure LE PQQ is OK but it's a bit pricey. $24.00 for 30 20mg caps on Amazon. That would be 6 days of this protocol at 100mg per dose.

 

ConsumerLab seems to be legit. It is listed on Consumer Reports as one of the labs that checks supplements. You need to be a paying member to see the full reviews, like the companies to avoid. It is $60 per year. I think I might sign up. There are some other supplements I want to check.

I did not renew my subscription to ConsumerLab because they also charge the companies to test there products.  So a company could have an excellent product, but would never be tested by ConsumerLabs if they were not a paying.

Re: post #2254

"So a company could have an excellent product, but would never be tested by ConsumerLabs if they were not a paying."

That is incorrect.

PAMPAGUY, read this webpage at ConsumerLab.com . It is only the "Quality Certification Program" that charges companies a fee. The "Product Reviews" are funded by member fees, etc (see the link). 

 "A company pays a testing fee for its product to be tested in the Quality Certification Program. This in no way guarantees the product will pass, nor does it mean that ConsumerLab.com won't select that product, or other products from that company, for future review." My emphasis.

Presumably the protocol reverses the epigenetic mtDNA damage detailed in this paper:

 https://www.mdpi.com...067/24/19/14858

Has anyone tried the protocol?  I did, but only for 1-2 days using the 150 mg PQQ protocol.  

Is it possible to reorder the updated information on the first page of the thread regarding the protocol? Where should we start for a first cycle? Thank you

Is it possible to reorder the updated information on the first page of the thread regarding the protocol? Where should we start for a first cycle? Thank you

Turnbuckle has a new post that looks at treating multiple sclerosis via removal of mitochondrial epimutations.

Turnbuckle has a new post that looks at treating multiple sclerosis via removal of mitochondrial epimutations.

Good results with 100 mg PQQ and 2g AAKG and 4 days later the same but with also 2g calcium lactate.

Now I'm not as fast as I used to be at 60 and perhaps with other issues, but as a baseline I ran 4x1200m at a 10 min/mi pace. I took the pqq/aakg/Ca lactate the day before a 1600m time trial and was able to do 8:42 minutes. I was running 6:30 10 years ago, but as a recent result I'm very pleased with my time.

Turnbuckle has a new post that looks at treating multiple sclerosis via removal of mitochondrial epimutations.

Worth reading because not only cured MS, but fatique also.  Another experience: A 70 year old female with fatigue and familial tremors took a single dose of 100 mg PQQ and 2 grams of AAKG. Her ability to repeatedly lift a dumbbell increased by more than 80% two hours later, and she noted that her tremors were substantially improved.

Should there AAKG be taken an hour ahead of the PQQ? I can't find info on the specifics for this new strategy.

There is a bit more info in the discussions section here: https://www.linkedin...dischler-snu5f/

There is a bit more info in the discussions section here: https://www.linkedin...dischler-snu5f/

Having looked at this does this now mean that the previous protocol could be replaced with the following?

Fission
5g Glycerol
2g AAKG
100mg PQQ

Fusion
2g lactate
2g AAKG
150mg PQQ

Would this be done on alternate days? Or do we still need to do the original protocol with NAM etc first?

cap3 - according to TB, you can do the treatment in one day.

First thing in AM:

3-5g GMS

4-5 hours later:

2-3g AAKG

120mg PQQ

I don't think you need the AAKG or PPQ for the fission stage.

My comments above do need confirmation, but that's my understanding as of now.

-T

cap3 - according to TB, you can do the treatment in one day.

 

First thing in AM:

3-5g GMS

 

4-5 hours later:

2-3g AAKG

120mg PQQ

 

I don't think you need the AAKG or PPQ for the fission stage.

 

My comments above do need confirmation, but that's my understanding as of now.

 

-T

What happened to the lactate to lock it in?

cap3 - according to TB, you can do the treatment in one day.

 

First thing in AM:

3-5g GMS

 

4-5 hours later:

2-3g AAKG

120mg PQQ

 

I don't think you need the AAKG or PPQ for the fission stage.

 

My comments above do need confirmation, but that's my understanding as of now.

 

-T

I saw that Turnbuckle mentions the protocol copied above, except that in the mention I see he refers to 'glycerol', not GMS.  He says, "Glycerol has a short half-life, so using it first thing in the morning can be followed an hour later by up-latching."  T, does Turnbuckle mention elsewhere that it's GMS he's taking as part of this single-day protocol, as opposed to glycerol?  

What happened to the lactate to lock it in?

My apologies - thanks for noticing that!  Just an accidental omission on my part:

First thing in AM:

3-5g GMS

2-3 hours later:

2-3g AAKG

120mg PQQ

Lactate (I take 6 "Sports  Legs" for 2G of Lactate.

I saw that Turnbuckle mentions the protocol copied above, except that in the mention I see he refers to 'glycerol', not GMS.  He says, "Glycerol has a short half-life, so using it first thing in the morning can be followed an hour later by up-latching."  T, does Turnbuckle mention elsewhere that it's GMS he's taking as part of this single-day protocol, as opposed to glycerol?  

GMS = glycerol monostearate

It has been previously discussed that this is the best form for the Stem Cell Protocol.

It's a good question as to whether GMS or straight glycerol is better for this protocol.

I'll see if I can find out.

cap3 - according to TB, you can do the treatment in one day.

 

First thing in AM:

3-5g GMS

 

4-5 hours later:

2-3g AAKG

120mg PQQ

 

I don't think you need the AAKG or PPQ for the fission stage.

 

My comments above do need confirmation, but that's my understanding as of now.

 

-T

GMS would be a fusion stage though. That's why I'm thinking he might mean a glycerol only product like this: https://a.co/d/dqDbFph.

GMS would be a fusion stage though. That's why I'm thinking he might mean a glycerol only product like this: https://a.co/d/dqDbFph.

stephen_b

I agree with you, thanks for continued engagement with this thread.  

I used to take glycerol as an athletic supplement and will be happy to reintroduce it.

Revised 11/05/2024

First thing in AM:

3-5g Pure Glycerol

1-2 hours later:

2-3g AAKG

120mg PQQ

Lactate (I take 6 "Sports  Legs" for 2G of Lactate)

This is still an evolving protocol

Yesterday I took the Beta Latching Protocol of:

5g glycerol

2 hours later:

2g AAKG

120mg PQQ

2g lactate salts from Sport legs

No side effects that I noticed.  I'll update how I feel over time.

Yesterday I took the Beta Latching Protocol of:

 

5g glycerol

 

2 hours later:

 

2g AAKG

120mg PQQ

2g lactate salts from Sport legs

 

No side effects that I noticed.  I'll update how I feel over time.

What brand of glycerol are you taking if you don't mind sharing the info?

What brand of glycerol are you taking if you don't mind sharing the info?

rarefried,

I used Gorilla brand from Amazon.

 

I tried glycerol (3g), waited 2 hours, then AAKG (5 ml)/PQQ (40 mg)/lactate (7.5 mg) a couple of days ago. I didn't notice much subjective benefit and running was not improved. The day after tried N+R (a gram of each), AKG (1 g), PQQ (40 mg) in the late afternoon and then GMS (5 ml)/Mg-stearate (5 ml)/AKG (5 ml)/lactate (7.5 ml) and PQQ (40 mg) the next morning. Lately I've been having trouble running 3 miles but today I ran 7.5 miles and felt stronger. I'm thinking of repeating the latter trial tomorrow but going entirely Mg-stearate, ditching the GMS entirely.

 

You need at least 100-120 mg PQQ.  Turnbuckle said that PQQ was the crucial supplement that made it all work.  High dose required.

 

I tried glycerol (3g), waited 2 hours, then AAKG (5 ml)/PQQ (40 mg)/lactate (7.5 mg) a couple of days ago. I didn't notice much subjective benefit and running was not improved. The day after tried N+R (a gram of each), AKG (1 g), PQQ (40 mg) in the late afternoon and then GMS (5 ml)/Mg-stearate (5 ml)/AKG (5 ml)/lactate (7.5 ml) and PQQ (40 mg) the next morning. Lately I've been having trouble running 3 miles but today I ran 7.5 miles and felt stronger. I'm thinking of repeating the latter trial tomorrow but going entirely Mg-stearate, ditching the GMS entirely.

 

stephen_b:

Thanks for this.  The note from Pampaguy is correct - the theoretical effectiveness of the unlatching protocol requires 120mg PQQ.

That said - I am doing this protocol mainly for the athletic benefits, so i am intrigued by your results.  If you carry on with a few more tests and get similar results, I'll give it a go and see if the results are replicated.