8 replies to this topic

[ageless]

Anyone try NADH supplementation? Supposedly has some impressive benefits for energy, dopamine synthesis, etc... Does it counteract the benefits of Benagene, which relies on the NAD+/NADH ratio?
I'm really confused on how it would affect that ratio, either positively or negatively.
Is NADH a promising supplement for those looking for healthy alternatives to modafinil and other energy/productivity aids?

[Brainbox]

A few years ago I took it for some months. I was not impressed at all. And it's fairly expensive.

I've not been looking into it lately, but as far as I know there's little scientific evidence on its efficacy.

[ageless]

Quote

A few years ago I took it for some months. I was not impressed at all. And it's fairly expensive.

I've not been looking into it lately, but as far as I know there's little scientific evidence on its efficacy.

Thanks brainbox. If I try it out I'll let you know what I think. I've read some interesting studies... not alot out there so that's why I'm asking people for their experiences.
Again, thanks.

[krillin]

Quote

Anyone try NADH supplementation? Supposedly has some impressive benefits for energy, dopamine synthesis, etc... Does it counteract the benefits of Benagene, which relies on the NAD+/NADH ratio?
I'm really confused on how it would affect that ratio, either positively or negatively.
Is NADH a promising supplement for those looking for healthy alternatives to modafinil and other energy/productivity aids?

Aubrey de Grey said it wouldn't be a good idea and pointed to this study on extracellular NADH causing lots of extracellular superoxide generation.

Biochem J. 1996 Sep 15;318 ( Pt 3):805-12.
High rates of extracellular superoxide generation by cultured human fibroblasts: involvement of a lipid-metabolizing enzyme.
O'Donnell VB, Azzi A.
Institute of Biochemistry and Molecular Biology, Bern, Switzerland.

    Expression of NADPH oxidase and low superoxide generation (approx. 0.06 nmol/min per 10(6) cells) by cytokine- or ionophore-stimulated human fibroblasts is known. However, we here show that these cells also contain an ectoplasmic enzyme, distinct from NADPH oxidase, which can generate superoxide (2.19 +/- 0.14 nmol/min per 10(6) cells) at levels similar to phorbol ester-stimulated monocytes on exogenous NADH addition. Superoxide generation was temperature-dependent, insensitive to chelation (desferal), and had a K(m) (app)(NADH) of 11.5 microM. Inhibitor studies showed that there was no involvement of NADPH oxidase (diphenylene iodonium, diphenyl iodonium), prostaglandin H synthase (indomethacin), xanthine oxidase (allopurinol), cytochrome P-450 (metyrapone) or mitochondrial respiration (rotenone, antimycin A). NAD+ was a competitive inhibitor, whereas NADPH supported 40% of the rate seen with NADH. No luminescence was observed after the addition of lactate, malate, pyruvate, GSH or L-cysteine. NADH-stimulated superoxide generation was enhanced by the addition of (3-30 microM) arachidonic acid, linoleic acid or (5S)-hydroxyeicosatetraenoic acid [(5S)-HETE] but not palmitic acid, (15S)-hydroperoxyeicosatetraenoic acid [(15S)-HPETE], (15S)-HETE or (12S)-HETE. Several features suggest involvement of an enzyme related to 15-lipoxygenase, and, in support of this, we show superoxide generation and NADH oxidation by recombinant rabbit reticulocyte 15-lipoxygenase. The large amounts of superoxide measured suggest that the fibroblast extracellular enzyme could be a major source of reactive oxygen species after tissue damage.

PMID: 8836123

[Shepard]

Yeah, seems pointless.

[meatwad]

I recall supplementing niacin is similar to supplying NAD+.

I also purchased some NAD+ (cheaper than NADH) and i couldn't recall much difference. Just take niacin ....

[krillin]

Quote

I also purchased some NAD+ (cheaper than NADH) and i couldn't recall much difference.

I wouldn't expect any, since any NAD+ would be reduced to NADH in the bloodstream.

[ageless]

Thanks for the replies... I keep you updated If I try this out. I still don't get this ratio thingy... is NADH bad for anti-aging whereas NAD+ is good, in terms of activating beneficial genes?
Some are saying that they are the same here?
So Ortho, do you get benefit from what you're doing? Is the DLPA safe and effective as it contains both the synthetic or mirror image of the natural form along with the natural L-phenylalanine form? Just doesn't seem like it would be best, but many say it does work well. I just find in most cases, the natural forms of nutrients are safer and more effective and the mirror image forms often toxic, harmful or at best, ineffective.