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Microglia Stem Cell Research Reports


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#1 caliban

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Posted 08 August 2011 - 09:25 PM


Attached File  mikisucess.png   19.34KB   0 downloads

Update June 2011:

After months of fundraising we are now delighted to announce that the project has started! Through many donations large and small, the community has raised sufficient funds to initiate the project. Last month, we passed the 80% mark and knew that full success would only be a matter of time.

Then, something amazing happened: though promoting this effort, a far sighted investor has stepped forward who can see the potential in developing this research project. The angel has been in touch with Mondey and committed a substantial contribution towards a research arm that is closely aligned to the project LongeCity is funding.

Thus, we have achieved something amazing together: every dollar donated to this life extension research project has not only been doubled through internal funds but multiplied manifold! Few people, especially those with very limited personal means, who want to invest in life extension would find such effective opportunities to make a real difference!
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#2 caliban

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Posted 08 August 2011 - 09:35 PM

Mondey has recently confirmed that the funding has been received and, in accordance with our funding conditions send me a few preliminary pictures from her lab. I'll leave her to comment further on these and more importantly the research progress when she gets a chance to stop by.


A 'bench' - surely not for sitting?
Attached File  slide0007_image010.jpg   8.76KB   10 downloads

Lots of cells in a vial:
Attached File  slide0007_image009.jpg   355.84KB   15 downloads

Lots of cells in the fridge:
Attached File  slide0007_image008.jpg   101.87KB   12 downloads

Just a few cells?
Attached File  slide0009_image014.jpg   12.86KB   10 downloads
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#3 AgeVivo

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Posted 08 August 2011 - 09:35 PM

Posted ImagePosted ImagePosted ImagePosted ImagePosted Image

#4 Mondey

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Posted 09 August 2011 - 11:59 AM

Dear community,
I wanted to thank everybody involved in organizing the science competition, to all of you who donated money and for all the comments, ideas, questions and general support.
We have started the project (money transfers between organizations and administration is slow ) and hope to make it an interesting project for all of you. I will keep you posted with regular up-dates on our progress and hope that you will learn with us and participate in the development of the project.

Thanks for your help and greetings Dey

PS: more pictures will be comming soon. The mice are ordered and in the moment we are differentiating the cells and establishing the immuno stainings.
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#5 Mondey

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Posted 29 August 2011 - 06:15 PM

Hi,
here are some stainings of our first transplantations:

1) Slice staining of a section of the brain with Major Histocompatibility Complex-II (MHC-II)
antibody which is marker for macrophage and dendritic cells.

Attached File  Slide1.JPG   12.16KB   0 downloads

2) Slice staining of a section of the brain with ionized calcium binding adaptor molecule 1
(Iba-1) antibody which is specifically expressed in macrophage/microglia and it is upregulated
during their activation.

Attached File  Slide2.JPG   13.22KB   0 downloads

3) Staining of slice of brain section with amyloid precursor protein (APP) antibody. Proteolysis
of APP results in the beta-amyloid which is the main component of the amyloid plaques in
the brain of Alzheimer patients.

Attached File  Slide3.JPG   11.37KB   1 downloads

4) APP/PS1 mouse in which bone marrow-derived microglia cells are transplanted. These main contain human mutant amyloid and presenelin. They get the first symptoms after around ~ 6-9 months.

Attached File  Slide4.JPG   21.79KB   1 downloads


More to come.


If anybody has experience in this kind of research and would like to exchange protocols or antibodies I would be very happy.

Greetings,
Dey
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#6 AgeVivo

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Posted 25 December 2011 - 09:32 PM

suggestion: This project should be highlighted at the frontpage and in the main pages, with a 3 line description (a little like whay was done for the laser project)
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#7 brokenportal

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Posted 09 March 2012 - 04:58 AM

This topic is currently in "Donor Hall of Fame". It should be in the list of science projects though shouldnt it? http://www.longecity...mmunityscience/

What is the latest news on this project?

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#8 Mind

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Posted 08 April 2012 - 02:12 PM

Just in case people did not see this promoted elsewhere: Here is a recent podcast with the lead researcher: http://www.longecity...andra-stolzing/




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