Located one study on my dosage question that found for mice:
... according to our dose-toxicity study, significant acute liver injury only occurred when the animals were administered a dose greater than 12.957 mg/kg, which is approximately 8 folds higher than the safety daily dose used clinically.
Suggesting the clinical dosage usually used with mice is 1/8 of 12.957 mg/kg = 1.62 mg/kg. The preparation methodology they used being alcohol extraction of the dried root. Just received the 10:1 extract I was looking for and it indicates water & alcohol extraction, which I assume yields a more full spectrum but lower purity extraction than the study extracting with alcohol alone. In the interests of caution I'll use the study number anyway as my upper limit for calculations.
The scaling factor I usually see for mouse to human dosage is 1/12. That brings the usual but well below danger level clinical dosage amount down to 0.135 mg/kg for humans. For me that comes out to 12.25 mg. That's quite a bit lower that the 100 mg I see in some of the combo commercial capsules... perhaps those preparations don't use 10:1 extract but dried root instead? Any thoughts, anyone?
Related to all this, Revgenetics use to market a product Anthony called "MasterGene P16" containing 4mg of Saikosaponin-A with a recommended dosage of 1 capsule every other week. And, if I remember correctly, he mentioned somewhere that it was a 50:1 Bupleurum extraction. Haven't seen it on their web site for years but how he extracted it is probably still his trade secret. But my research-reading suggests the Saikosaponins (A & D) are soluble in dmso. Gonna have to dig a little more into that since the studies that show Bupleurum benefits to liver health identify Saikosaponins as being responsible.
Howard
