Hi All,
I'm trying to design an experiment of my res plasma concentration. I have access to HPLC instruments but wanted to see what other people thought about my experimental design.
First I we sort of already know that Res has very low bioavailability from other animal and human published studies.
1st off typical bioavailability calculations use a formula that requires an intravenous application of the drug and an oral administration. Well I'm not going to do IV infusion of Res. Sorry…
So I thought if I make a linear standard curve of Res then hopefully my plasma concentration will fit some where on that curve. If it does I can extrapolate how much actually got into my blood stream.
I would be following this method.
Determination of trans-Resveratrol in Plasma by
HPLC
Anal. Chem. 1999, 71, 747-750
My idea is the Piperine has showed to facilitate bioavailability of other compounds but I can't find any data for Piperine and Res?
On a fasting stomach I would take 10mg of Bioperine wait for 30mins for it to inhibit the degradation pathways of res. After 30mins or T=0 for Res administration I would take a sample of my blood as a blank for the HPLC run. This will insure there are no interfering peaks. I will attempt to take samples at T=10,20,30,45,60,120min. I need to have some blood left J
Hopefully I will get an absorbance that will fit on the linear curve. Also the time points will allow use to so if Bioperine stably inhibits the degradation of res.
Your thoughts are more then welcome.