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Implanting an immortalized bone marrow AND a thymus to replace old cells by immortalized young cells

htert bone marrow stem cells thymus immune system telomerase immortalized cells senescence cells replacement implanting bone marrow yamanaka factors

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#1 VicenteSilvaNeyra

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Posted 24 September 2020 - 10:15 PM


I am working on algorithms to let computers help us to achieve indefinite life extension. 
I have come out with the next idea and I would like to meet people interested in collaborating with myself and other people to explore different approaches to accomplish rejuvenation and immortality. 
 

Immortalized human cells can be created with complementary DNA (cDNA) to code for the hTERT protein to induce telomerase activity (along with other methods)

 

Simonsen, JL, Rosada, C, Serakinci, N, et al have extended the lifespan proliferation of human bone marrow.

"The transduced cells have now undergone more than 260 population doublings (PD) and continue to proliferate, whereas control cells underwent senescence-associated proliferation arrest after 26 PD"

 

On the other hand, there are several approaches for thymus regeneration (Valentin P. Shichkin & Mariastefania Antica 2020) and artificial thymic organoids (reference below)

 

What would be the challenges of implanting an immortalized bone marrow AND a thymus in animals?

 

I think that by doing this, the immune system would get rid of senescence cells to be replaced by new immortalized cells created in the immortalized bone marrow. In this way, the longer Hayflick limit of immortalized cells would keep regenerated organs and tissue healthier for longer, including the thymus itself, which in turns would continue killing senescence cells to extend youth and lifespan of the whole body.

 

What if we partially express Yamanaka factors (along with other factors) to rejuvenate cells first and then express telomerase activity to make them both younger and “immortal”?

 

When we get old, both bone marrow and thymus stop working, then, by replacing old cells with younger cells with higher telomerase activity might lead to both rejuvenation and indefinite lifespan. 

 

Is anybody already working on a similar approach?

What happens when immortalized cells are put inside an old animal?

What is the risk of doing this?

What is missing to get this done?

 

The procedure of implanting a bone marrow would be done once, because it would generate immortal cells that could replace the old cells of the body, whereas injecting vectors would only work partially. 

 

In case you are interested, what would be the best days and times for having a quick chat on skype to discuss this and other ideas?

 

Vicente

 

Skype:    

fider_69@hotmail.com

 

LinkedIn

https://www.linkedin...neyra-67990118/

 

References:
 

Simonsen, JL, Rosada, C, Serakinci, N, et al. Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells. Nat Biotechnol. 2002;20(6):592–596

https://pubmed.ncbi....h.gov/12042863/

 

Valentin P. Shichkin & Mariastefania Antica. Thymus Regeneration and Future Challenges

file:///C:/Users/Mi%20Pc/Downloads/Shichkin-Antica2020_Article_ThymusRegenerationAndFutureCha.pdf

 

Artificial thymic organoids represent a reliable tool to study T-cell differentiation in patients with severe T-cell lymphopenia

https://ashpublicati...sent-a-reliable

Attached Files



#2 VicenteSilvaNeyra

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Posted 04 October 2020 - 03:25 PM

Employing vectors to produce mutations using CRISPr has a low impact on the human body. There is scientific literature about the small percentage of cells that get benefit from this technique.

Another possibility is creating a bone marrow which generates immortalized stem cells inside a body, which might mean that the new cells with higher telomerase activity will be replacing senescence cells with the help of the immune system. 

 

Old people have lost both the thymus and the production of stem cells. 

 

If we live a maximum lifespan of 120 years with a Hayflick limit of 40-60 divisions, then if the cells of our organs, tissue and blood can divide around 200 times, it might mean that the whole body would age at a very slow rate. 

 

These are some of the experiments that could be carried out:

- Create several clones of a single mouse embryo.

- Half of the embryos are immortalized through hTERT mutation, tumor suppressors genes, any other method or a combination of them. 

- The other half of the mice are genetically modified to age faster.

- When the second set gets old, they receive the bone marrow of the mice that have a higher Hayflick limit. Because all mice are clones, then the immune system should accept the immortalized bone marrow.

 

Questions:

- Will the old mice get younger and extend their lifespan?

- Is the thymus regenerated when there is a source of stem cells?

- What would happen if both thymus and bone marrow from immortalized mice are implanted in the older ones? would they be rejuvenated?

- Instead of immortalized mice, would it be possible to produce embryos and mice without a brain and nervous system? so that the immortalized set of mice provides the older clones with a source of bone marrow, thymus and other immortalized organs?.

- Is it possible to create human bodies without brains? so these brainless clones of people are made of immortalized cells and their bone marrow can be implanted in the old people who need them? 

- Which of these experiments have been already carried out?

 

I would appreciate it if we collaborate by sharing relevant information that positively gets us closer to the goal of immortality and rejuvenation.

 

Thanks for appreciating life and health more than anything else.

 

Vicente

silvaneyra (at) gmail . com



#3 VicenteSilvaNeyra

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Posted 11 October 2020 - 08:51 PM

How can we minimize the risk of stem cell infusion?

 

What is the main cause of the risk involved in stem cell infusion?

 

How can we make the whole process more efficient?

 

Anyone interested in splitting the work of doing research?

 

Possibly, we can build a team and make progress on this subject.

 

Risk according to the FDA:

https://www.fda.gov/consumers/consumer-updates/fda-warns-about-stem-cell-therapies

 

Some possible side effects:

  • Cancer
  • Administration site reactions,
  • The ability of cells to move from placement sites and change into inappropriate cell types or multiply,
  • Failure of cells to work as expected, and
  • The growth of tumors.
  • Serious infections
  • Blindness


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Also tagged with one or more of these keywords: htert, bone marrow, stem cells, thymus, immune system, telomerase, immortalized cells, senescence cells replacement, implanting bone marrow, yamanaka factors

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