4 replies to this topic

[Kevinsan]

The Thais have RED_GEM. What is it? Apparently can rejuvenate cells. Rumor in the papers is that they tried it on humans. They have similar results on dogs. What are they and how do they work? Seems similar to WNT manipulation to reverse aging in individual cells. Chulu is their top university. Their sponsor is their largest oil company - PTT.

 

https://www.chula.ac...ighlight/59580/

 

Chula Medical Breakthrough! RED-GEM Molecules to Reverse Aging

“RED-GEMs’ ability to reverse aging is the key solution to health problems in an aging society and an income generator for Thailand as well,” said Prof. Dr. Apiwat, a specialist in molecular genetics and epigenetics.

The discovery of Replication independent endogenous DNA double strand breaks  1 (DNA gaps) that are rare in aging cells 3 is the key to our knowledge in rejuvenation.

“Youth DNA Gaps are found where there is DNA methylation — the origin of RED-GEMs which can protect the DNA and prevent genes from aging,” explains Prof. Dr. Apiwat.

“When people grow older, DNA gaps decrease, causing tension across the DNA strands.  The DNA cannot rotate normally and is easily destroyed. We therefore find more DNA lesions or damage in the aging cells. These DNA lesions signal cells to stop dividing normally, and start to age, as well as mutate and become cancerous.”

“RED-GEMs can help increase DNA gaps, remove DNA lesions, and fix the aging of cells,” Prof. Dr. Apiwat explained.

Success! Reverse aging in lab mice

Prof.Dr. Apiwat detailed the RED-GEMs experiments with 3 groups of mice: 7-month-old, 30-month-old, and 3-month-old.

“Researchers dyed aging cells blue. Seven-month-old mice hardly had any aging cells, while older mice (30 months old) were full of aging cells in their livers.  The elderly mice that received RED-GEMs once a week for 2 months, had decreased number of aging cells in their liver,” said Prof. Dr. Apiwat.

“This experiment was not to destroy aging cells but to reverse them into normal functioning cells. It was also found that brain functioning abilities of old mice that received the RED-GEMs was restored as well.”

The experiments also found that diseases found in aging cells, such as senescence-associated proteins, visceral fat, and liver fibrosis also decreased while Youth DNA Gap increased.  This means the cells are rejuvenated.  In addition, RED-GEMs were also tested with mice with burn wounds, and mice with diabetic wounds and yielded great results. The wounds healed faster and the flesh became softer and firmer.

[Mind]

Interesting, however, it would be nice to know what the Red-Gems are.

[smithx]

One of them is defined in this paper:
https://www.research...-2140452/v1.pdf

Apparently they used a plasmid to induce the Box A domain of the protein HMGB1, and are claiming that this domain rejuvenates cells:

 

Both principle mechanism of action and treatment outcome of both Alu-siRNA and box A of HMGB1 expression plasmid are unprecedented. Both molecules protect DNA(9, 17, 54, 77, 78). For example, the DNA of cells pretreated by box A had higher resistance to radiation than untreated cells(9). We classied these two molecules as rejuvenating DNA by genomic stability molecules (REDGEM). Rejuvenating DNA or RED means cells treated with REDGEM are added youth-associated epigenetic marks. Genomic stability molecule or GEM means REDGEM protects DNA from being damaged.

Alu or SINE-siRNA is not promising for future clinical use. In addition to AGO4, the siRNA may incorporate into other AGOs and yield different outcomes among different cell types. In addition, SINE loci distribution and sequences among animal species are distinct. So SINE methylation outcome of each species may be different. Therefore, preclinical evaluation of SINE-siRNA may not be fully equivalent to Alu-siRNA.

 

We showed that Box A rejuvenated cells by producing DNA gaps to increase the durability of old DNA(9). The strengthened DNA eventually reduced endogenous DNA damage and DDR. Consequently, Box A transfected cells’ senescent signaling cascade was limited and as a result cells were rejuvenated (Fig. 6). We currently demonstrated that Box A expression plasmid transfection could rejuvenate senescent cells in two models of aging rats: d-galactose-induced aging and natural-aging rats(9). Box A improved memory and liver function and reduced visceral fat, liver brosis, and senescence-associated proteins. The rejuvenation degree was remarkable because Box A reversed all aging markers to that observed in youth groups(9). These experiments indicated that youth-DNA-gap reduction is the nidus of the aging process and the cellular senescence stage is maintained by youth-DNA-gap reduction(9). Improving memory means Box A is a promising therapeutic approach for senile dementia(9). The reduction of brosis expands the treatment potential of Box A to numerous pathological structures in the extracellular space, such as lung brosis, amyloid in brain, and fat deposits in arteriosclerosis(9). Interestingly, HMGB1 was shown to prevent and ameliorate heart hypertrophy by inhibiting DDR(90, 91). Therefore, producing DNA gaps may be valuable for treating DNA damage- and age-associated diseases or conditions, including major organ failure(9).

 

 

 

 

[Kevinsan]

Here is the explanation. Maybe someone can explain it to me. Two links - a PDF and Youtube.

 

 

https://www.nstda.or...ที่เลือกได้.pdf

Edited by Kevinsan, 28 June 2023 - 04:57 AM.

[smithx]

They used a plasmid to induce the Box A domain of the protein HMGB1, and are claiming that this domain rejuvenates cells by making breaks in DNA, which they are calling "youth DNA gaps". They claim that young cells have more DNA gaps (or breaks) than older cells and by inducing more of them with Box A they rejuvenate the cell.

 

It seems sort of crazy because DNA breaks are not generally thought of as a good thing at all. In fact, quite the reverse. If true it's new to me.

 

That's the summary!