10 replies to this topic

[zoolander]

Comp Biochem Physiol B Biochem Mol Biol. 2007 Jan 31;

    Protective effect of Withania somnifera (Solanaceae) on collagen glycation and cross-linking.
        * Babu PV,
        * Gokulakrishnan A,
        * Dhandayuthabani R,
        * Ameethkhan D,
        * Kumar CV,
        * Ahamed MI.

    Department of Biochemistry, Islamiah College, Vaniyambadi 635 752, Tamil Nadu, India.

    Modification of collagen such as non-enzymatic glycation and cross-linking plays an important role in diabetic complications and age-related diseases. We evaluate the effect of Withania somnifera on glucose-mediated collagen glycation and cross-linking in vitro. Extent of glycation, viscosity, collagen-linked fluorescence and pepsin solubility were assessed in different experimental procedures to investigate the effect of W. somnifera. Tail tendons obtained from rats (Rattus norvegicus) weighing 250-275 g were incubated with 50 mM glucose and 100 mg of metformin or Withania root powder or ethanolic extract of Withania under physiological conditions of temperature and pH for 30 days. Formation of advanced glycation end products (AGE) was measured by fluorescent method whereas the cross-linking of collagen was assessed by pepsin digestion and viscosity measurements. Tendon collagen incubated with glucose showed an increase in glycation, AGE and cross-linking of collagen. The collagen incubated with W. somnifera and metformin ameliorates these modifications. The ethanolic extract of Withania showed more prominent effect than Withania root powder. The activity of ethanolic extract of Withania is comparable to metformin, a known antiglycating agent. In conclusion, Withania could have therapeutic role in the prevention of glycation induced pathogenesis in diabetes mellitus and aging.

    PMID: 17329138 [PubMed - as supplied by publisher]

[health_nutty]

Zoo, have you seen any in vivo studies?

[Karomesis]

interesting link. thanks Zoo. [glasses]

[DukeNukem]

Ashwagandha is what is being referred to in this article?

[zoolander]

Yes. Ashwagandha.

[xanadu]

Tail tendons from rats weighing 250 to 275 grams? That's almost 10 oz, a whole rat might weigh that much and this is just the tendon from the tail? Man, I'd hate to come up against the rat that came from in a dark alley!

All the more reason to take ashwaganda which I do. Of course there is a gap between in vivo and in vitro. I wonder how much you have to take to get those effects?

[wayside]

I think its

Tail tendons from (rats weighing weighing 250 to 275 grams),

not

(Tail tendons weighing 250 to 275 grams) from rats.

Either that, or the total weight of all the tendons from all the rats weighed 250-275 grams, its a little ambiguous.

[health_nutty]

Lots of supplements are anti-glycating in vitro. Any reason to believe that Ashwaganda will be more effective in vivo than the others? I'm asking because I'm genuinely curious.

Green tea:
http://www.ncbi.nlm....l=pubmed_docsum

Pomegranate:
http://www.ncbi.nlm....anite glycation

Ginseng:
http://www.ncbi.nlm....l=pubmed_docsum

Garlic:
http://www.ncbi.nlm....l=pubmed_docsum

[maxwatt]

Comp Biochem Physiol B Biochem Mol Biol. 2007 Jan 31;

    Protective effect of Withania somnifera (Solanaceae) on collagen glycation and cross-linking.
   

I cannot access this; perhaps if I upgraded my membership?

[opales]

Comp Biochem Physiol B Biochem Mol Biol. 2007 Jan 31;

    Protective effect of Withania somnifera (Solanaceae) on collagen glycation and cross-linking.
   

I cannot access this; perhaps if I upgraded my membership?

Yes it's in full member forum.

[zoolander]

Yes it's in full member forum.

Yes. With lots of other goodies