I have a big bush of excellent culinary rosemary in my garden. Would anyone care to hazard a guess as what would be an adequate dose of minced fresh rosemary to obtain the antiglycative effects described in the abstract below?
J. Agric. Food Chem., ASAP Article 10.1021/jf0631833 S0021-8561(06)03183-9
Web Release Date: March 27, 2007
Copyright © 2007 American Chemical Society
Low-Density Lipoprotein, Collagen, and Thrombin Models Reveal that Rosemarinus officinalis L. Exhibits Potent Antiglycative Effects
Chiu-Lan Hsieh, Chiung-Huei Peng, Charng-Cherng Chyau, Yuh-Charn Lin, Hui-Er Wang, and Robert Y. Peng*
Department of Food and Nutrition, Research Institute of Biotechnology, and Division of Basic Medical Sciences, Hung-Kuang University, No. 34 Chung-Chie Road, Shalu County, Taichung Hsien, Taiwan 43302
Received for review November 5, 2006. Revised manuscript received February 23, 2007. Accepted February 28, 2007.
Abstract:
Using the low-density lipoprotein (LDL), collagen, and thrombin models, we report here that the rosemary extracts (REs), either the aqueous (REw) or the acetonic (REA), all possessed many antiglycation-related features, and the effective concentrations required were as follows: 0.1 mg/mL for suppressing the relative electrophoretic mobility, 1.3 g/mL for anticonjugated diene induction, 0.5 mg/mL for inhibition of thiobarbituric acid reactive substances production, 0.1 mg/mL for AGEs (advanced glycation end products) formation, 0.1 mg/mL to block glucose incorporation, and 0.05 mg/mL as an effective anti-antithrombin III. Using high-performance liquid chromatography/mass spectrometry, we identified five major constituents among eight major peaks, including rosmarinic acid, carnosol, 12-methoxycarnosic acid, carnosic acid, and methyl carnosate. In the LDL model, REA was proven to be more efficient than REw; yet, the reverse is true for the collagen and the thrombin III models, the reason of which was ascribed to the higher lipid-soluble antioxidant content (such as rosmarinic acid, carnosol, carnosic acid, 12-methoxycarnosic acid and methyl carnosate) in REA than in REw and the different surface lipid characteristics between LDL and collagen; although to act as anti-AGEs, both extracts were comparable. To assist the evidence, a larger 2,2-diphenyl-1-picrylhydrazyl radical scavenging capability with less total polyphenolic content was found in REA. We conclude that rosemary is an excellent multifunctional therapeutic herb; by looking at its potential potent antiglycative bioactivity, it may become a good adjuvant medicine for the prevention and treatment of diabetic, cardiovascular, and other neurodegenerative diseases.
Keywords: Rosemary; anti-thrombin; collagen; low-density lipoprotein; glycation
Edited by steelheader, 08 April 2007 - 08:33 PM.
