To anyone with access to the full article: how much exercise did the rats do?
Rejuvenation Res. 2007 Dec 10 [Epub ahead of print] Links
Exercise Training Promotes SIRT1 Activity in Aged Rats.Ferrara N,
Rinaldi B, Corbi G, Conti V, Stiuso P, Boccuti S, Rengo G, Rossi F,
Filippelli A.
Department of Health Sciences, Faculty of Medicine, University of
Molise, Campobasso, Italy., S. Maugeri Foundation, Scientific
Institute of Telese Terme, Telese Terme, Italy.
The objective of this study was to determine the effects of aging and
exercise training on SIRT1 activity and to identify a pathway linking
SIRT1 to antioxidant response and cell cycle regulation in rats. SIRT1
is a NAD(+)-dependent deacetylase involved in the oxidative stress
response and aging. The effects of aging and of moderate and prolonged
exercise training in rats are unknown. We measured SIRT1 activity in
heart and adipose tissue of young (6 months old), sedentary old (24
months), and trained old (24 months) rats using an assay kit.
Peroxidative damage was determined by measuring levels of
thiobarbituric reactive substances (TBARS) and the protein-aldehyde
adduct 4-hydroxynonenal (4-HNE). MnSOD, catalase, and FOXO3a levels
were evaluated by Western blot, and GADD45a, cyclin D(2), and FOXO3a
mRNA by RT-PCR. Aging significantly reduced SIRT1 activity in heart,
but not in adipose tissue, increased TBARS and 4-HNE and decreased Mn-
SOD and catalase expression in both heart and adipose tissue. Aging
did not affect FOXO3a protein expression in the heart or FOXO3a mRNA
in adipose tissue. Exercise training significantly increased FOXO3a
protein in the heart and FOXO3a mRNA in adipose tissue of aged rats.
It also significantly increased Mn-SOD and catalase levels in both
heart and adipose tissue. The exercise-induced increase in SIRT1
activity in the heart caused a decrease in cyclin D(2) and an increase
in GADD45a mRNA expression. There was a similar decrease in cyclin
D(2), and no changes in GADD45a mRNA expression in adipose tissue. We
concluded that exercise training, which significantly increases SIRT1
activity, could counteract age-related systems impairment.
PMID: 18069916 [PubMed - as supplied by publisher]
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