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Resveratrol Chem Tests (purity) update


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#91 edward

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Posted 22 February 2008 - 02:12 AM

http://www.pathway2c...eratrol/SEM/VP/

If somebody wants to repost the images on the forum go for it.



As you can tell there are a lot of clumps but they don't look like large cyrstals rather a lot of small particles smashed together. There are a few larger particles and you can see that the surface looks much different.

Very interesting.


Yes I agree very interesting, I wonder if the large particles are really just clumps of the smaller particles which due to their size, possible static charge and maybe moisture content are simply clumped together and will break up or alternatively at the center of these big clumps is a larger particle serving as a "core that is attracting all the small particles kind of like a comet with a solid rock or ice core attracts intra stellar dust and grows in size, yeah yeah extreme analogy but you get the idea.

#92 niner

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Posted 22 February 2008 - 04:57 AM

http://www.pathway2c...eratrol/SEM/VP/

If somebody wants to repost the images on the forum go for it.


As you can tell there are a lot of clumps but they don't look like large cyrstals rather a lot of small particles smashed together. There are a few larger particles and you can see that the surface looks much different.

Very interesting.

Yes I agree very interesting, I wonder if the large particles are really just clumps of the smaller particles which due to their size, possible static charge and maybe moisture content are simply clumped together and will break up or alternatively at the center of these big clumps is a larger particle serving as a "core that is attracting all the small particles kind of like a comet with a solid rock or ice core attracts intra stellar dust and grows in size, yeah yeah extreme analogy but you get the idea.

It looks to me like it was very finely powdered at one time, but then it caked due maybe to the solvent not having been sufficiently removed. Then the cake was repowdered mechanically. The small particles look fluffy, not the hard crystal morphology that we saw from the other samples. Could this be from spray drying?

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#93 Hedgehog

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Posted 22 February 2008 - 05:12 AM

It looks to me like it was very finely powdered at one time, but then it caked due maybe to the solvent not having been sufficiently removed. Then the cake was repowdered mechanically. The small particles look fluffy, not the hard crystal morphology that we saw from the other samples. Could this be from spray drying?


I wouldn't rule out that the crystals didn't grow correctly as in there is an impurity that doesn't allow the crystals to grow very large. I wonder if Revgenetics sample and VP have the same melting point which might clue us into how the crystaline nature. VP sample was also yellowish hinting at an impurity and I really wanted to find one on the HPLC results but I didn't.

I guess I could also try to recrystallize the sample and see what they look like? Humm maybe I will try this tomorrow. Any ideas as to a solvent to use? I was thinking maybe a very non-polar organic solvent? maybe hexane or heptane. If it really is water then the resveratrol should be absorbed into the organic layer. Ideas?

the current sample reminds me of a dried slushy.

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#94 niner

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Posted 22 February 2008 - 05:34 AM

It looks to me like it was very finely powdered at one time, but then it caked due maybe to the solvent not having been sufficiently removed. Then the cake was repowdered mechanically. The small particles look fluffy, not the hard crystal morphology that we saw from the other samples. Could this be from spray drying?


I wouldn't rule out that the crystals didn't grow correctly as in there is an impurity that doesn't allow the crystals to grow very large. I wonder if Revgenetics sample and VP have the same melting point which might clue us into how the crystaline nature. VP sample was also yellowish hinting at an impurity and I really wanted to find one on the HPLC results but I didn't.

I guess I could also try to recrystallize the sample and see what they look like? Humm maybe I will try this tomorrow. Any ideas as to a solvent to use? I was thinking maybe a very non-polar organic solvent? maybe hexane or heptane. If it really is water then the resveratrol should be absorbed into the organic layer. Ideas?

the current sample reminds me of a dried slushy.

People have used EtOAc/hexane (1:1), MeOH, EtOH, EtOH/water... I guess it would depend on what you have on hand, and what you wanted to do with the crystals later. I'd expect recrystalization to yield much larger crystals, and probably take care of the coloration. Activated charcoal might take care of the brownish stuff too.

#95 ilanso

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Posted 24 February 2008 - 07:34 AM

I'm not a member but I took a look at the Yahoo group. WOH! The fight between Revgeneric and Biotrivia is getting real nasty. On top of that, the name of Sinclair was being taken in vain.


It's happening again in http://www.napavalle...5f774117264.txt :

phytoman wrote on Feb 22, 2008 1:15 PM:
" The compound resveratrol has shown interesting cardioprotective properties in clinical trials. Drs. Anderson and Setia reported in a January paper that of 121 subjects with moderate to severe loss of heart function those patients given biotivia Transmax, an extract of red wine used by researchers, 94 showed marked improvement in cholesterol levels, reduced inflammation and improved circulation. Resveratrol works by activating the human Sirt 1,2,3 genes much like caloric restriction does. Sirtris pharma is developing a synthetic version of transmax which it intends to have on the market in five years. In another study by Dr. Sinclair of Harvard published in the journal Nature in November transmax was shown to increase the life span of obese mammals by 31%. This is an exciting area of research and could lead to some revolutionary new preventative strategies and safer less invasive treatments for heart disease. I Dr. Sinclair's study of obese mice he found that those fed resveratrol lived 31% longer and were free of cardiovascular diseases."



#96 docmaas

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Posted 24 February 2008 - 10:00 AM

I'm not a member but I took a look at the Yahoo group. WOH! The fight between Revgeneric and Biotrivia is getting real nasty. On top of that, the name of Sinclair was being taken in vain.


It's happening again in http://www.napavalle...5f774117264.txt :

phytoman wrote on Feb 22, 2008 1:15 PM:
" The compound resveratrol has shown interesting cardioprotective properties in clinical trials. Drs. Anderson and Setia reported in a January paper that of 121 subjects with moderate to severe loss of heart function those patients given biotivia Transmax, an extract of red wine used by researchers, 94 showed marked improvement in cholesterol levels, reduced inflammation and improved circulation. Resveratrol works by activating the human Sirt 1,2,3 genes much like caloric restriction does. Sirtris pharma is developing a synthetic version of transmax which it intends to have on the market in five years. In another study by Dr. Sinclair of Harvard published in the journal Nature in November transmax was shown to increase the life span of obese mammals by 31%. This is an exciting area of research and could lead to some revolutionary new preventative strategies and safer less invasive treatments for heart disease. I Dr. Sinclair's study of obese mice he found that those fed resveratrol lived 31% longer and were free of cardiovascular diseases."


So is phytoman James Betz? Whoever he is he seems to think or desires to dissemble that transmax is a synonym for resveratrol. If it is James this is just plain dishonest and he is playing with fire if he is continuing to do it. If not phytoman should be informed of his lack of accuracy in his terminology.

Mike

#97 inawe

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Posted 24 February 2008 - 01:08 PM

In the land of France, a child was born under miraculous circumstances. Three genies, Res, Vera and Trol, came bearing diapers, formula and polyphenols. They baptized the child with the name Paradox. The French Paradox gave the neighbors the good news: "you can pig out all you want as long as you chase it down with vino".
At a later day, Biotrivia stumbled upon platinum tablets written in ancient Phoenician. The angel Rosetta appeared and translated it in no time. It had the recipe for mixing some powder with the French Paradox myth and transform it into gold.

#98 Anthony_Loera

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Posted 24 February 2008 - 04:56 PM

I'm not a member but I took a look at the Yahoo group. WOH! The fight between Revgeneric and Biotrivia is getting real nasty. On top of that, the name of Sinclair was being taken in vain.


It's happening again in http://www.napavalle...5f774117264.txt :

phytoman wrote on Feb 22, 2008 1:15 PM:
" The compound resveratrol has shown interesting cardioprotective properties in clinical trials. Drs. Anderson and Setia reported in a January paper that of 121 subjects with moderate to severe loss of heart function those patients given biotivia Transmax, an extract of red wine used by researchers, 94 showed marked improvement in cholesterol levels, reduced inflammation and improved circulation. Resveratrol works by activating the human Sirt 1,2,3 genes much like caloric restriction does. Sirtris pharma is developing a synthetic version of transmax which it intends to have on the market in five years. In another study by Dr. Sinclair of Harvard published in the journal Nature in November transmax was shown to increase the life span of obese mammals by 31%. This is an exciting area of research and could lead to some revolutionary new preventative strategies and safer less invasive treatments for heart disease. I Dr. Sinclair's study of obese mice he found that those fed resveratrol lived 31% longer and were free of cardiovascular diseases."


So is phytoman James Betz? Whoever he is he seems to think or desires to dissemble that transmax is a synonym for resveratrol. If it is James this is just plain dishonest and he is playing with fire if he is continuing to do it. If not phytoman should be informed of his lack of accuracy in his terminology.

Mike


Brainbox,
I understand your reluctance regarding certain posts I have made about James regarding stuff like this. How should I address this sort of post, with facts or not at all? I believe last time I answered you erased the post. Maybe you can help us understand what James has mentioned in his personal Im's regarding this situation.

A

#99 edward

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Posted 24 February 2008 - 05:38 PM

In the land of France, a child was born under miraculous circumstances. Three genies, Res, Vera and Trol, came bearing diapers, formula and polyphenols. They baptized the child with the name Paradox. The French Paradox gave the neighbors the good news: "you can pig out all you want as long as you chase it down with vino".
At a later day, Biotrivia stumbled upon platinum tablets written in ancient Phoenician. The angel Rosetta appeared and translated it in no time. It had the recipe for mixing some powder with the French Paradox myth and transform it into gold.


lol

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#100 edward

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Posted 24 February 2008 - 05:41 PM

I'm not a member but I took a look at the Yahoo group. WOH! The fight between Revgeneric and Biotrivia is getting real nasty. On top of that, the name of Sinclair was being taken in vain.


It's happening again in http://www.napavalle...5f774117264.txt :

phytoman wrote on Feb 22, 2008 1:15 PM:
" The compound resveratrol has shown interesting cardioprotective properties in clinical trials. Drs. Anderson and Setia reported in a January paper that of 121 subjects with moderate to severe loss of heart function those patients given biotivia Transmax, an extract of red wine used by researchers, 94 showed marked improvement in cholesterol levels, reduced inflammation and improved circulation. Resveratrol works by activating the human Sirt 1,2,3 genes much like caloric restriction does. Sirtris pharma is developing a synthetic version of transmax which it intends to have on the market in five years. In another study by Dr. Sinclair of Harvard published in the journal Nature in November transmax was shown to increase the life span of obese mammals by 31%. This is an exciting area of research and could lead to some revolutionary new preventative strategies and safer less invasive treatments for heart disease. I Dr. Sinclair's study of obese mice he found that those fed resveratrol lived 31% longer and were free of cardiovascular diseases."


This is just despicable. Who else could it be but someone with a vested interest in Transmax. I guess someone could be really mislead/confused, but how do you think they got that way...

#101 ilanso

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Posted 24 February 2008 - 06:40 PM

I'm not a member but I took a look at the Yahoo group. WOH! The fight between Revgeneric and Biotrivia is getting real nasty. On top of that, the name of Sinclair was being taken in vain.


It's happening again in http://www.napavalle...5f774117264.txt :

phytoman wrote on Feb 22, 2008 1:15 PM:
" The compound resveratrol has shown interesting cardioprotective properties in clinical trials. Drs. Anderson and Setia reported in a January paper that of 121 subjects with moderate to severe loss of heart function those patients given biotivia Transmax, an extract of red wine used by researchers, 94 showed marked improvement in cholesterol levels, reduced inflammation and improved circulation. Resveratrol works by activating the human Sirt 1,2,3 genes much like caloric restriction does. Sirtris pharma is developing a synthetic version of transmax which it intends to have on the market in five years. In another study by Dr. Sinclair of Harvard published in the journal Nature in November transmax was shown to increase the life span of obese mammals by 31%. This is an exciting area of research and could lead to some revolutionary new preventative strategies and safer less invasive treatments for heart disease. I Dr. Sinclair's study of obese mice he found that those fed resveratrol lived 31% longer and were free of cardiovascular diseases."


This is just despicable. Who else could it be but someone with a vested interest in Transmax. I guess someone could be really mislead/confused, but how do you think they got that way...


Could he have tried to deliberately disinform to the extent that transmax is an extract of red wine, when its label clearly states Polygonum C.?

#102 niner

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Posted 25 February 2008 - 04:52 AM

Brainbox,
I understand your reluctance regarding certain posts I have made about James regarding stuff like this. How should I address this sort of post, with facts or not at all? I believe last time I answered you erased the post. Maybe you can help us understand what James has mentioned in his personal Im's regarding this situation.

Anthony, not to speak for Brainbox but I for one appreciate your input regarding this situation, which I find frankly psychopathic. However, a lot of people want to avoid "flamewars" between vendors in the halls of imminst. Some set the bar for what is a flamewar a lot lower than I do, but that's ok, it's just how forums are. If it's any consolation, I think everyone is on the same page about how appallingly sleazy this is.

#103 inawe

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Posted 25 February 2008 - 03:33 PM

Some low lifes are perpetuating long disproved myths for
financial gain. That's why it's very important for well respected
researchers to speak out and set the record straight. In a rear
interview prof. Johan Auwerx cathegorically stated: "There is no
thread of evidence that the so called French paradox is due to the
favorite French pastime of chewing Polygonum Cuspidatum".

#104 Anthony_Loera

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Posted 25 February 2008 - 04:12 PM

Brainbox,
I understand your reluctance regarding certain posts I have made about James regarding stuff like this. How should I address this sort of post, with facts or not at all? I believe last time I answered you erased the post. Maybe you can help us understand what James has mentioned in his personal Im's regarding this situation.

Anthony, not to speak for Brainbox but I for one appreciate your input regarding this situation, which I find frankly psychopathic. However, a lot of people want to avoid "flamewars" between vendors in the halls of imminst. Some set the bar for what is a flamewar a lot lower than I do, but that's ok, it's just how forums are. If it's any consolation, I think everyone is on the same page about how appallingly sleazy this is.


Psychopathic? ouch.. maybe a bit harsh. Specific 'marketing' may not fall under that term, my nephew who appears to suffer from this issue (and drives me and my son nuts) really doesn't care how he gets attention.

A

#105 niner

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Posted 26 February 2008 - 05:20 AM

Brainbox,
I understand your reluctance regarding certain posts I have made about James regarding stuff like this. How should I address this sort of post, with facts or not at all? I believe last time I answered you erased the post. Maybe you can help us understand what James has mentioned in his personal Im's regarding this situation.

Anthony, not to speak for Brainbox but I for one appreciate your input regarding this situation, which I find frankly psychopathic. However, a lot of people want to avoid "flamewars" between vendors in the halls of imminst. Some set the bar for what is a flamewar a lot lower than I do, but that's ok, it's just how forums are. If it's any consolation, I think everyone is on the same page about how appallingly sleazy this is.


Psychopathic? ouch.. maybe a bit harsh. Specific 'marketing' may not fall under that term, my nephew who appears to suffer from this issue (and drives me and my son nuts) really doesn't care how he gets attention.

A

Read this fascinating portrait of psychopathy. It was kind of an eye opener for me. Most of you probably know at least one psychopath. They're not all Hannibal Lecter or Jeffrey Dahmer. Some are very charming. Also very successful.

#106 Anthony_Loera

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Posted 26 February 2008 - 03:10 PM

Hehehe...

I love this line...

For those who can't be bothered with such expensive frills, we present the PCL-DI: an alternative, PCL-inspired test guaranteed to appear scientific.


Thats it, my nephew is a psychopath... :)
A

#107 lokiboy2

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Posted 21 February 2009 - 06:09 AM

Want to see a real war?

Sign out of ImmInst and then log on again to this page

See Resveratrol Banner above and four RES Ads below....



I know absolutely nothing about chemistry, but please allow me to put my two cents in from 1st hand experience with pomegranate extract and resveratrol. I began taking resveratrol and pomegranate extract 4 months ago and notice a significant decrease in the psoriatic lesions on the trunk and lower parts of my body. Unfortunately I ran out of resveratrol and didn't make the connection that the combination was probably the result in the increased anti-inflammatory effect. Recently I purchased Transmax and I've been taking that and pomegranate extract for the last 3 days now. I hope it's not an imposition, but I'd really like to get your opinion on the combined effect of both of the supposed ant-inflammatories? Are there any studies which have confirmed my experience with the combination? Any help you can give this novice would be appreciated greatly. Anyone out there? please help. I'm stumbling around in the dark here trying to turn this disease around.

#108 lokiboy2

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Posted 21 February 2009 - 06:11 AM

Want to see a real war?

Sign out of ImmInst and then log on again to this page

See Resveratrol Banner above and four RES Ads below....



I know absolutely nothing about chemistry, but please allow me to put my two cents in from 1st hand experience with pomegranate extract and resveratrol. I began taking resveratrol and pomegranate extract 4 months ago and notice a significant decrease in the psoriatic lesions on the trunk and lower parts of my body. Unfortunately I ran out of resveratrol and didn't make the connection that the combination was probably the result in the increased anti-inflammatory effect. Recently I purchased Transmax and I've been taking that and pomegranate extract for the last 3 days now. I hope it's not an imposition, but I'd really like to get your opinion on the combined effect of both of the supposed ant-inflammatories? Are there any studies which have confirmed my experience with the combination? Any help you can give this novice would be appreciated greatly. Anyone out there? please help. I'm stumbling around in the dark here trying to turn this disease around.

#109 gloria yin

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Posted 16 March 2009 - 07:11 AM

dear sir:

thank you for you useful imformation .you HPLC photographe can SEM picture.
Can you show me the RESV HPLC analytical method ?and have you bought any other resv 99% or 98%from china vendor?
Thank you!

gloria


On Friday I put the samples on GC and HPLC. I watched the Vitalprime labs GC analysis for the first 10mins and I didn't see any residue solvents. Total run time is 30mins.

I took pic of the appearance test

http://www.pathway2c...om/resveratrol/

Update:

Relative Strengths Finished

http://www.pathway2c...ve_strength.xls

GC Results (tested for Ethanol, Methanol, Acetone, Dichloromethane), RT = Retention Time

1) All samples potentially had a minor EtOH peak. The retention time wasn't exact but pretty close (RT standard 2.963, samples 2.974-2.988) 2) All samples contained a larger peak at 4.28 but my standard didn't contain anything close enought to that RT.

Uniformity Results

Product

% of Label Claim Result

Trans-Max(1)

91.3%

Trans-Max(2)

102.8%

R500

112.7%

Bioforte(1)

103.8%

Bioforte(2)

113.9%

Images of uniformity chromatographs

http://www.pathway2c...rol/Res_PDF.pdf



SEM IMAGES Resveratrol.

For high quality images vist http://www.pathway2c...sveratrol/SEM/

Posted Image

Rev 99 50X

Posted Image

Trans-Max 50x

Posted Image

Rev99 100X

Posted Image

Trans-Max 100x

Posted Image

Rev 99 250X

Posted Image

Trans-Max 250X

Posted Image

Rev99 1000X

Posted Image

Trans-Max 1000X



#110 Hedgehog

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Posted 17 March 2009 - 04:25 PM

dear sir:

thank you for you useful imformation .you HPLC photographe can SEM picture.
Can you show me the RESV HPLC ?and have you bought any other resv 99% or 98%from china vendor?
Thank you!

gloria


I got the HPLC method from a published paper and is the same method that Sirtris pharma uses. No i haven't bought any from a the China vendor

#111 michae4

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Posted 17 March 2009 - 09:33 PM

I got the HPLC method from a published paper and is the same method that Sirtris pharma uses.


Would you mind sharing the name/authors of this paper?

#112 Hedgehog

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Posted 17 March 2009 - 10:25 PM

I got the HPLC method from a published paper and is the same method that Sirtris pharma uses.


Would you mind sharing the name/authors of this paper?



Is is for the blood plasma analysis..... Or go to pubmed and the keywords I have used are "HPLC Resveratrol plasma"



http://cebp.aacrjour.../full/16/6/1246


Sample Preparation
After defrosting, plasma and urine samples were extracted with acidified methanol [5% methanol with 2% acetic acid (pH 4), equal volume for plasma, twice the volume for urine]. The extractant was centrifuged (9, 000 x g, 15 min) and the supernatant was analyzed. Fecal matter was homogenized (5-mL water containing 2% phosphoric acid) and the homogenate was centrifuged (4°C, 2,900 x g, 15 min). Analyte was isolated from the supernatant using solid-phase extraction (Oasis HLB, Waters; volume, 3 mL; packing weight, 30 mg). Solid-phase cartridges were primed (1-mL acidified methanol followed by 1-mL water), washed (1 -mL acidified methanol), and analyte was eluted with methanol. While urine extractant and column eluents obtained from fecal extracts were injected onto the high-performance liquid chromatography column directly, plasma extractant was dried under nitrogen and reconstituted in mobile phase before high-performance liquid chromatography analysis. Resveratrol content in stool was related to dry feces weight, which was obtained by drying (100°C) stool to weight constancy (24 h).

High-Performance Liquid Chromatography Analysis
Resveratrol and its metabolites were extracted and separated using a gradient UV-high-performance liquid chromatography system (Waters Breeze) as described before (29). Separation was achieved on a Waters Atlantis C18 column (4.6 x 150 mm, 3 µm; Waters) in combination with a Waters Atlantic C18 guard column (4.6 x 20 mm, 5 µm). Column oven temperature was 35°C (flow rate, 1 mL/min). The gradient elution system (A: 5 mmol/L ammonium acetate; B: 98% methanol, both with 2% propan-2-ol) was as follows with respect to B: 0 to 7 min 20%, 7 to 16 min 50%, 16 to 18 min 55%, 18 to 23 min 95%, then reequilibration to 0% B for 6 min before the next injection. Injection volume was 100 µL. The retention time of resveratrol was 18.6 min; its lower limit of detection was 5 ng/mL. This method has been validated for resveratrol in terms of interday and intraday variability, recovery, accuracy, and precision (29). As resveratrol metabolites were not available in sufficient quantities for method development, their quantities were calculated based on the assumption that recovery characteristics and relationship between peak area ratios and concentrations were the same as those for resveratrol. Metabolite concentrations are therefore described as "resveratrol equivalents." Authentic resveratrol 3-sulfate was provided by Royalmount Pharma. The identity of resveratrol and its metabolites was confirmed using an Agilent 1100 series high-performance liquid chromatography with in-line Applied Biosystems/MDS SCIEX API 2000 ion spray triple quadrupole mass spectrometer (Applied Biosystems) using the same chromatographic conditions as described above.

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#113 felice

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Posted 16 May 2009 - 08:17 AM

I got the HPLC method from a published paper and is the same method that Sirtris pharma uses. No i haven't bought any from a the China vendor


Dear Sir,
Could you tell us the name of the China vendor(s) (even better their contact info)?
I live in Taiwan and my best friend is in China. Both of his parents work at
a Chinese pharmaceutical company and I've asked them to look for some quality
sources I can buy from long term. Thanks~

Felice

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#114 2tender

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Posted 16 May 2009 - 11:13 PM

Felice, in case your are interested there is a quality vendor in the ad at the top of this page. They test every batch and I think they ship worldwide.




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