The high concentration resveratrol formulation (150 mM SRT501 in 40% Captisol (w/v) on a 500 mL scale) was prepared as follows. A 40% Captisol (w/v) was prepared by placing 560 g of Captisol (CyDex, Lot # CY-04A-05006) into a 4 L beaker. A large stir bar was added to the beaker that was then placed on a large stir plate. In-house Milli-Q water was added to bring the volume in the beaker to ˜1.3 L. The solution was mixed vigorously for 1 hour until clear. At times, a spatula was used to free the stir bar and dislodge undissolved material from the sides of the vessel. The solution was transferred to a 2 L graduated cylinder. Approximately 100 mL of water was used to rinse the beaker and was added to the graduated cylinder, bringing the final volume to 1.4 L. The solution was mixed thoroughly until clear.
Captisol = Cyclodextrin
You can buy parts of this on my website. I'm not selling resveratrol only the pieces to make their formulation.
http://www.baresveratrol.com (will soon be transferred over to Vital Prime)
Thier formulation works well I have done a plasma test. However, it takes a lot of stirring to actually get the molecules to complex (even then I probably only get 40-60% to complex). So if you want to try to make it fill free to get the ingrediants. This formulation will allow you to get a quick and very fast plasma spike. I personally haven't had any problems with this formulation I have one report of a upset stomach.
As a full discloser Vital Prime is taking my other two formulations and I'm only acting as a consultant. On a personal note my personal endeavors have turned me back onto the Hedgehog pathway (the reason for my Screen name). I have found a naturally occurring inhibitor of this pathway and it targets the pathway at the best location possible! I already have a few labs stating they want the compound ASAP to start Vivo models. To make a long story short if this compound really inhibits it as stated in a very recent paper we will probably have one of the world's top naturally occurring anti-cancer compounds....
Hopefully this will be a major break through. I should have about 3 data sets from three different labs on this compound by the end of summer. Wish me LUCK 
I haven't been this x-cited about the Hh pathway in a VERY long time if you can't tell. BTW one of these labs works with preclinical animals models w/ GenentechThe high concentration resveratrol solution was prepared by mixing 600 mL of 40% Captisol in a 1 L beaker with 24.0 g resveratrol and stirring vigorously (the target concentration would be 175 mM resveratrol in 40% Captisol (w/v) if all the material dissolved). The solution was stirred for 35 minutes, followed by sonication for 1 hour. The solution was stirred for an additional 50 minutes, followed by sonication for an additional hour. At this point, solid material was still present in the sample. An aliquot was removed for analysis. The pH was measured at 4.76 and the concentration was measured by HPLC to be 55 mM resveratrol. Because the solution appeared cloudy at this time, three portions were separated to try alternate mixing methods. Approximately 200 mL was transferred to a 500 mL volumetric flask and was sonicated for 45 min, approximately 300 mL was vigorously mixed with an overhead mixer for 90 minutes, and approximately 100 mL was homogenized for 25 minutes. None of the three portions appeared clear. A sample of the homogenized solution was removed for concentration analysis and had a concentration of 74 mM resveratrol. The portions were combined and were stirred at ambient temperature overnight protected from light. The following morning, using an aseptic technique in the laminar flow hood, the solution was sterile filtered via a Nalgene 90 mm filter unit (Cat# 167-0020; 0.02 PES membrane). The concentration was again measured and determined to be 168 mM resveratrol in 40% Captisol.
The control vehicle (40% Captisol (w/v)) was also prepared as follows. 700 mL of 40% Captisol was transferred into a 1 L beaker. The solution was sonicated for a total of 2 hours. The solution was then stored at ambient temperature overnight. Using an aseptic technique in the laminar flow hood, the solution was sterile filtered via a Nalgene 90 mm filter unit (Cat# 167-0020; 0.02 PES membrane).
Edited by Hedgehog, 19 April 2008 - 12:29 AM.
