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Successful preservation of neural cells


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#1 JonesGuy

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Posted 07 November 2003 - 02:43 AM


Well, I'm a cryobiology student, so this is right up my alley. I've known this was possible, but I didn't realise it was possible yet. The process needs quite a bit of fine-tuning, but ehn, that's what grants are for.

PS: I recommend www.onelook.com for your dictionary needs.

from the journal Cryobiology Volume 47, Issue 2 , October 2003, Pages 179-183

Cryopreservation of human brain tissue allowing timely production of viable adult human brain cells for autologous transplantation

Abstract
Background. Autologous transplantation is an attractive approach to treat some neurological diseases. A major obstacle is the capacity to produce cells for transplantation at the appropriate time. We describe a cryopreservation procedure for adult human brain tissue allowing the generation of cells in vitro.

Methods. Neurological resections were dissected to separate white and grey matter. Fractions were frozen in a specific cryopreservation medium containing a selected serum and stored in liquid nitrogen. Tissue was thawed, cells were mechanically dissociated, expanded in culture and characterized by immunochemistry.

Results. Adult human brain tissue cryopreserved for up to two years was successfully used to generate brain cells that could be maintained in culture for up to 100 days. Cells expressed a variety of neuroectodermal markers including GFAP, S100, and neurofilament.

Conclusion. A successful procedure for cryopreservation of adult human brain tissue has been established that might facilitate future autologous transplantation strategies.

#2 kevin

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Posted 07 November 2003 - 03:02 AM

well well..

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