21 replies to this topic

[youandme]

http://www.imminst.o...ity-t33963.html
Thought it would be better to ask this in the Res forum !

JAK inhibitors currently hold promise as anti cancer and anti inflammatory compounds..a JAK2 inhibitor is in Phase 2 for RA

Emodin is also a potent JAK2 inhibitor. Also Resveratrol has been stated to regulate JAK.

The 2 may perhaps be synergistic against Auto-Inflammation...and in any case Resveratrol is the only source of Emodin I can find !!

So please can anyone suggest a quality supplier of high content Emodin Resveratrol Supplement ?!

I realise less pure Res is normally frowned upon however Emodin has some interesting abilites worth pursuing for me personally.

Thanks

[Anthony_Loera]

You do know a high amount of Emodin, will make you run to the bathroom, right?

The whole issue about emodin, is that high amounts will produce diarrhea. That is the reason most folks want the lowest amount amount of emodin in their resveratrol supplements.



A

[2tender]

High Emodin content products can be tolerated for a brief period, without the GI tract stimulation, in some people. Once the sensitivity develops though, it becomes instant upon ingestion. Some people have no problem with it whatsoever, and some people prefer it for that reason, as evidenced by the fact there are still high emodin content products available. I would say that any product thats half pure would meet your needs. There is a list here somewhere.

[maxwatt]

Rheum emodi is a plant source of emodin. So is buckthorn bark. These can be found in some laxatives. For instance, this one contains buckthorn bark.

If you want to use the Rheum emodi plant, capsules containing a 5:1 powder extract is available at Chinese Herbs Direct. However I cannot tell you the precise amount of emodin these pills contain, nor can I personally vouch for their quality though I've no reason to doubt it.

Vitacost NSI band used to have a 20% resveratrol product that also was labeled as 20% emodin. I don't know if it is still available. But chronic use of emodin may lead to ulceration of the colon, and may even cause colon cancer.

If you are looking for low dose, Japanese knotweed is the main source of resveratrol and can be found growing wild in every state but Hawaii. A tea can be made from the dried leaves and sliced and dried roots, which will contain some emodin and resveratrol. Steeping it with alcohol (i.e., vodka) would increase the content, though it will still be low. The tea is said by some herbalists to be a mild laxative, and to contribute to health.

[youandme]

Thanks for your post Maxwatt

Over here and Europe 'Rhubarb' is the name of the edible plant containing emodin.

You mentioned that emodin is carcinogenic for the colon...I did a search came back with a study showing anti cancer properties for the human colon..so not sure about that ?! all the studies I can find so far indicate it has anti cancer properties.
http://cat.inist.fr/...cpsidt=20322491

The Effect of Emodin on VEGF Receptors in Human Colon Cancer Cells

Im happy to try emodin with Res..as a trial.
Of course dosage is what one can cope with...Im working on the principle of any amount of JAK2 inhibition in me is perhaps better than it is at the moment.

So 50% res brands with some emodin is perhaps where I would start.

Rheum emodi is a plant source of emodin. So is buckthorn bark. These can be found in some laxatives. For instance, this one contains buckthorn bark.

If you want to use the Rheum emodi plant, capsules containing a 5:1 powder extract is available at Chinese Herbs Direct. However I cannot tell you the precise amount of emodin these pills contain, nor can I personally vouch for their quality though I've no reason to doubt it.

Vitacost NSI band used to have a 20% resveratrol product that also was labeled as 20% emodin. I don't know if it is still available. But chronic use of emodin may lead to ulceration of the colon, and may even cause colon cancer.

If you are looking for low dose, Japanese knotweed is the main source of resveratrol and can be found growing wild in every state but Hawaii. A tea can be made from the dried leaves and sliced and dried roots, which will contain some emodin and resveratrol. Steeping it with alcohol (i.e., vodka) would increase the content, though it will still be low. The tea is said by some herbalists to be a mild laxative, and to contribute to health.

[youandme]

High Emodin content products can be tolerated for a brief period, without the GI tract stimulation, in some people. Once the sensitivity develops though, it becomes instant upon ingestion. Some people have no problem with it whatsoever, and some people prefer it for that reason, as evidenced by the fact there are still high emodin content products available. I would say that any product thats half pure would meet your needs. There is a list here somewhere.

Thanks for you post 2tender

I realised that they have been selling this for ages with emodin..so it cant be so much of a problem.
At least I can try.
Perhaps I would be lucky enough to not have the sensitivity issue.


Im not sure of the list you talking about ?

Cheers

[youandme]

You do know a high amount of Emodin, will make you run to the bathroom, right?

The whole issue about emodin, is that high amounts will produce diarrhea. That is the reason most folks want the lowest amount amount of emodin in their resveratrol supplements.



A

Thanks for that !

Emodin has some very interesting properties other than the D side effect.
I just want to try and see if it helps me with Autoimmunity and perhaps more.
Res seems to have mixed reviews for Autoimmunity so far..so 50% res Im happy to try if it has quite a bit of Emodin in the mix.
Res Purists need not read this thread

And of course one bonus 50% res is cheaper.
Its worth a try for me.

Just need a reputable brand where I can trust that the ingredients are what they claim to be in the container.



Also of course ..If anyone knows of another JAK Inhibtor in food or suuplements please do let me know


Cheers

[2tender]

High Emodin content products can be tolerated for a brief period, without the GI tract stimulation, in some people. Once the sensitivity develops though, it becomes instant upon ingestion. Some people have no problem with it whatsoever, and some people prefer it for that reason, as evidenced by the fact there are still high emodin content products available. I would say that any product thats half pure would meet your needs. There is a list here somewhere.

Thanks for you post 2tender

I realised that they have been selling this for ages with emodin..so it cant be so much of a problem.
At least I can try.
Perhaps I would be lucky enough to not have the sensitivity issue.


Im not sure of the list you talking about ?

Cheers

I would'nt take high Emodin content for very long, or every day it can contribute to GI erosion, you will see, its self-limiting in use. The list is in the vendors section, green bar at the top of the page, you really shouldnt click on it.

[2tender]

Sorry, its under the green box at the top of the page in Pinned: Suppliers list

[youandme]

I would'nt take high Emodin content for very long, or every day it can contribute to GI erosion, you will see, its self-limiting in use. The list is in the vendors section, green bar at the top of the page, you really shouldnt click on it.

Thanks for the warnings..I hope they are on the packaging that the resveratrol comes with.
At least I know before I start.

I would probably take the Res/Emodin mix once a week to start...and see how we go...dosage will be as it says on the packaging..gotta start somewhere.

I shouldnt have but I have clicked on the link...!

Now if anyone ever hear's or know's of another potential JAK inhibitor please do let us know

Edited by youandme, 08 February 2010 - 12:25 AM.

[youandme]

Going thru all the suppliers in the list...no one seems to state the Emodin content....perhaps because of the side effects !

Found one supplier who states 10% Emodin and 20% Resveratrol

Is this a reputable supplier guys ?

NutraceuticsRX

http://www.vitasprin...aceuticsrx.html

Edited by youandme, 08 February 2010 - 01:43 AM.

[2tender]

If you want to get whatever benefits emodin may have, it should probably be taken EOD, then stop when you experience bowel intolerance. Cycled like that may work better. If you purchase the cheapest Res. you can find, it should have enough emodin for you needs. Just my thoughts.

[Anthony_Loera]

The issue is, and has been that resveratrol with emodin, causes bowel issues... some very bad ones for some folks.

If that company you mention has high emodin content, I would advise most folks to to stay away from it. Looking at the label... it appears it has 50mg of emodin per capsule. 50mg! per 100mg resveratrol capsule...

From a commercial perspective, that is a horrible way to start a relationship with new customers... take a pill, run to the bathroom? Heck no, I could not do this to our customers, not even as a joke....

A

[youandme]

A

Believe me Autoimmunity is no "Joke"

[Anthony_Loera]

youand me... Neither is crapping on yourself.

I once asked a customer this hypothetical question (we were talking about bubble boy, rapamycin, and other things)
I asked "If this drug made you run to the bathroom, would you prefer to have the runs and live longer, or not have the runs and live life as you know it.... "

His answer was: to live life without the runs... he then told me he had cancer.


I believe most folks would answer the same way.

[youandme]

Thanks A



Getting back on topic.

Please could anyone verify if this supplier is a reputable..or not

NutraceuticsRX

Thanks

Edited by youandme, 10 February 2010 - 03:06 AM.

[Anthony_Loera]

Do they have a COA on the website?

If they don't (which I can't find one, do you?) then it appears they are less forthcoming than others on the resveratrol list:
http://www.imminst.o...ers-t36436.html

Of course, you want emodin... so buy it and tell us about it.

[youandme]

Thanks A for your responses in this thread, certainly understand your post intentions very clearly.

[maxwatt]

Thanks A for your responses in this thread, certainly understand your post intentions very clearly.

I am familiar with the knotweed extract that contains 20% resveratrol and 10% emodin. I'd check the manufacture date on the bottles, though the extract could be much older than the date of encapsulation.

Some people can tolerate as much as 60mg of emodin without complaint, but most people are very uncomfortable after taking 30 mg or more. I'd start with the lowest possible dose of a single capsule for a few days, and increase by that amount every few days. You'll know when you've reached your limit.

Emodin shows some desirable effects with cell cultures in the lab, but as yet no one has found them with people. Long term use may cause hepatitis.

Curcumin is probably a better alternative as a JAK2 inhibitor:

Curcumin regulates signal transducer and activator of transcription (STAT) expression in K562 cells

Biochemical Pharmacology
Volume 72, Issue 11, 30 November 2006, Pages 1547-1554

Romain Blasiusa, Simone Reutera, Estelle Henrya, Mario Dicatoa and Marc Diederich, Laboratoire de Biologie Moléculaire et Cellulaire du Cancer, Hôpital Kirchberg, 9, Rue Edward Steichen, L-2540 Luxembourg, Luxembourg
Received 14 April 2006; accepted 31 July 2006. Available online 7 September 2006.

Abstract
Signal transducers and activators of transcription (STATs) play important roles in numerous cellular events as for example differentiation, inflammation or immune response. Furthermore, constitutive STAT activation can be observed in a high number of tumors. In our hands, curcumin treatment induced a decrease of nuclear STAT3, -5a and -5b, without affecting neither STAT1, nor the phosphorylation state of STAT1, -3 or -5 in the K562 cell line. Most interestingly, the decrease of nuclear STAT5a and -5b after curcumin treatment was accompanied by an increase of truncated STAT5 isoforms, indicating that curcumin is able to induce the cleavage of STAT5 into its dominant negative variants lacking the STAT5 C-terminal region. Interferon (IFN)-β and -γ treatment induced IFN-stimulated responsive element (ISRE) transcriptional activity, which was efficiently inhibited by curcumin pre-treatment. In parallel, IFN-γ treatment induced an increase of the amount of nuclear STAT1 and -3, as well as their phosphorylated isoforms. Again, curcumin pre-treatment inhibited these increases. Finally, curcumin treatment inhibited Jak2 mRNA expression as well as cyclin D1 and v-src gene expression in K562 chronic leukaemia cells.

Edited by maxwatt, 12 February 2010 - 10:34 AM.

[youandme]

Wow, first I heard of Curcumin being a JAK2 inhibitor..!

Is JAK2 inhibition Curcumins sole anti-cancer anti-inflammatory mechanism..or has it more ?

Good Find

Thanks Maxwatt !

Now what kind of dosage could we use for effective inhibition....anybody's guess ....I guess

Curcumin is probably a better alternative as a JAK2 inhibitor:

Curcumin regulates signal transducer and activator of transcription (STAT) expression in K562 cells

Biochemical Pharmacology
Volume 72, Issue 11, 30 November 2006, Pages 1547-1554

Romain Blasiusa, Simone Reutera, Estelle Henrya, Mario Dicatoa and Marc Diederich, Laboratoire de Biologie Moléculaire et Cellulaire du Cancer, Hôpital Kirchberg, 9, Rue Edward Steichen, L-2540 Luxembourg, Luxembourg
Received 14 April 2006; accepted 31 July 2006. Available online 7 September 2006.

Abstract
Signal transducers and activators of transcription (STATs) play important roles in numerous cellular events as for example differentiation, inflammation or immune response. Furthermore, constitutive STAT activation can be observed in a high number of tumors. In our hands, curcumin treatment induced a decrease of nuclear STAT3, -5a and -5b, without affecting neither STAT1, nor the phosphorylation state of STAT1, -3 or -5 in the K562 cell line. Most interestingly, the decrease of nuclear STAT5a and -5b after curcumin treatment was accompanied by an increase of truncated STAT5 isoforms, indicating that curcumin is able to induce the cleavage of STAT5 into its dominant negative variants lacking the STAT5 C-terminal region. Interferon (IFN)-β and -γ treatment induced IFN-stimulated responsive element (ISRE) transcriptional activity, which was efficiently inhibited by curcumin pre-treatment. In parallel, IFN-γ treatment induced an increase of the amount of nuclear STAT1 and -3, as well as their phosphorylated isoforms. Again, curcumin pre-treatment inhibited these increases. Finally, curcumin treatment inhibited Jak2 mRNA expression as well as cyclin D1 and v-src gene expression in K562 chronic leukaemia cells.

Edited by youandme, 13 February 2010 - 05:47 AM.

[maxwatt]

chemical and Biophysical Research Communications
Volume 388, Issue 2, 16 October 2009, Pages 377-382

Curcumin activates AMPK and suppresses gluconeogenic gene expression in hepatoma cells

Teayoun Kima, Jessica Davisa, Albert J. Zhanga, Xiaoming Hea and Suresh T. Mathews, a,
aDepartment of Nutrition and Food Science, Boshell Diabetes and Metabolic Diseases, Research Program, Auburn University, Auburn, AL 36849, USA
Received 27 July 2009. Available online 8 August 2009.
Abstract
Curcumin, the bioactive component of curry spice turmeric, and its related structures possess potent anti-oxidant and anti-inflammatory properties. Several lines of evidence suggest that curcumin may play a beneficial role in animal models of diabetes, both by lowering blood glucose levels and by ameliorating the long-term complications of diabetes. However, current understanding of the mechanism of curcumin action is rudimentary and is limited to its anti-oxidant and anti-inflammatory effects. In this study we examine potential anti-diabetic mechanisms of curcumin, curcumin C3 complex®, and tetrahydrocurcuminoids (THC). Curcuminoids did not exert a direct effect on receptor tyrosine kinase activity, 2-deoxy glucose uptake in L6-GLUT4myc cells, or intestinal glucose metabolism measured by DPP4/α-glucosidase inhibitory activity. We demonstrate that curcuminoids effectively suppressed dexamethasone-induced phosphoenol pyruvate carboxy kinase (PEPCK) and glucose6-phosphatase (G6Pase) in H4IIE rat hepatoma and Hep3B human hepatoma cells. Furthermore, curcuminoids increased the phosphorylation of AMP-activated protein kinase (AMPK) and its downstream target acetyl-CoA carboxylase (ACC) in H4IIE and Hep3B cells with 400 times (curcumin) to 100,000 times (THC) the potency of metformin. These results suggest that AMPK mediated suppression of hepatic gluconeogenesis may be a potential mechanism mediating glucose-lowering effects of curcuminoids.
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The Journal of Immunology, 1999, 163: 3474-3483.

Curcumin Blocks Cytokine-Mediated NF-B Activation and Proinflammatory Gene Expression by Inhibiting Inhibitory Factor I-B Kinase Activity1

Christian Jobin2,*,, Cynthia A. Bradham*,, Maria Pia Russo*,, Booker Juma§, Acharan S. Narula¶, David A. Brenner*,, and R. Balfour Sartor*,
Departments of * Medicine, Microbiology, and Immunology, Biochemistry and Biophysics, and Center for Gastrointestinal Biology and Disease, University of North Carolina, Chapel Hill, NC 27599; § Department of Natural Sciences, Fayettsville State University, Fayettesville, NC, 28301; and ¶ Narula Research, Chapel Hill, NC 27516


Abstract

NF-B plays a critical role in the transcriptional regulation of proinflammatory gene expression in various cells. Cytokine-mediated activation of NF-B requires activation of various kinases, which ultimately leads to the phosphorylation and degradation of IB, the NF-B cytoplasmic inhibitor. The food derivative curcumin has been shown to inhibit NF-B activity in some cell types. In this report we investigate the mechanism of action of curcumin on cytokine-induced proinflammatory gene expression using intestinal epithelial cells (IEC). Curcumin inhibited IL-1ß-mediated ICAM-1 and IL-8 gene expression in IEC-6, HT-29, and Caco-2 cells. Cytokine-induced NF-B DNA binding activity, RelA nuclear translocation, IB degradation, IB serine 32 phosphorylation, and IB kinase (IKK) activity were blocked by curcumin treatment. Wound-induced p38 phosphorylation was not inhibited by curcumin treatment. In addition, mitogen-activated protein kinase/ERK kinase kinase-1-induced IL-8 gene expression and 12-O-tetraphorbol 12-myristate 13-acetate-responsive element-driven luciferase expression were inhibited by curcumin. However, IB degradation induced by ectopically expressed NF-B-inducing kinase or IKK was not inhibited by curcumin treatment. Therefore, curcumin blocks a signal upstream of NF-B-inducing kinase and IKK. We conclude that curcumin potently inhibits cytokine-mediated NF-B activation by blocking a signal leading to IKK activity.
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Curcumin down-regulates AR gene expression and activation in prostate cancer (37). Inhibitory effects of curcumin on c-Jun/AP-1 and/or NF-κB activation have been observed in both ...
www.charakinternational.com/pdfs/article5.pdf
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[Anthony_Loera]

Sounds like someone maybe considering some Curcumin, instead of emodin now....

Good find Maxwatt

A