I have been wondering whether or not Doxycline has some sort of notropic value, I've been on it for a infected scratch for a week now and I feel very clear headed. I found this abstract and it had me thinking perhaps there is a longevity value in taking Doxycline every so often?
control of gene expression in vitro and in vivo
J K Koponen1, H Kankkonen1, J Kannasto1, T Wirth1, W Hillen2, H Bujard3 and S Ylä-Herttuala1
1A.I.Virtanen Institute and Department of Medicine, University of Kuopio, Kuopio, Finland
2Institut für Mikrobiologie, Universität Erlangen, Germany
3Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Germany
Correspondence: S Ylä-Herttuala, Department of Molecular Medicine, A.I. Virtanen Institute, University of Kuopio, POB 1627, FIN-70211 Kuopio, Finland
Received 7 June 2002; Accepted 13 August 2002.
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Abstract
Regulated expression of therapeutic genes is required for long-term gene therapy applications for many disorders. Here we describe a doxycycline (dox)-regulated lentiviral vector system consisting of two HIV-1-based self-inactivating viruses. One of the vectors is constitutively expressing a novel improved version of the tetracycline reverse transactivator rtTA2S-M2 and the other has a rtTA responsive promoter driving the expression of -galactosidase gene (lacZ). The rtTA2S-M2 has highly improved properties with respect to specificity, stability and inducibility. Functionality of the system by dox was confirmed after in vitro cotransduction of Chinese hamster ovary and human endothelial hybridoma (EAhy926) cells. Regulation of the system showed tight control of the gene expression. Dose dependence for dox was seen with concentrations that can be obtained in vivo with doses normally used in clinical practice. LacZ expression could be switched on/off during long-term (3 months) culturing of cotransduced cells. The system was next tested in vivo after cotransduction into rat brain and studying expression of the lacZ gene in dox-treated and control rats. Nested RT-PCR confirmed that the tight control of the gene expression was achieved in vivo. Also, X-gal staining showed positive cells in the dox-treated rats, but not in the controls 10 days after cotransduction with 4 days preceding treatment with dox. It is concluded that our doxycycline-regulated vector system shows significant potential for long-term gene therapy treatments.
source
http://www.nature.co...s/3301889a.html
