8 replies to this topic

[ihatesnow]

http://www.whec.com/...3.shtml?cat=565
http://www.physorg.c...dna-stress.html

Edited by ihatesnow, 17 June 2011 - 07:57 PM.

[ihatesnow]

http://www.dailymail...s.html?ITO=1490

[Elus]

http://www.dailymail...s.html?ITO=1490

That's my Lab. I work at the Gorbunova lab. Lemme know if you have any questions (I'm an undergrad there, so I can direct questions to the researchers as well)

Edited by Elus, 17 June 2011 - 09:10 PM.

[niner]

Here's the abstract:

Nature. 2011 Jun 15;474(7351):395-8. doi: 10.1038/nature10165.
Converting nonsense codons into sense codons by targeted pseudouridylation.
Karijolich J, Yu YT.

Department of Biochemistry and Biophysics, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, New York 14642, USA.

All three translation termination codons, or nonsense codons, contain a uridine residue at the first position of the codon. Here, we demonstrate that pseudouridylation (conversion of uridine into pseudouridine (Ψ), ref. 4) of nonsense codons suppresses translation termination both in vitro and in vivo. In vivo targeting of nonsense codons is accomplished by the expression of an H/ACA RNA capable of directing the isomerization of uridine to Ψ within the nonsense codon. Thus, targeted pseudouridylation represents a novel approach for promoting nonsense suppression in vivo. Remarkably, we also show that pseudouridylated nonsense codons code for amino acids with similar properties. Specifically, ΨAA and ΨAG code for serine and threonine, whereas ΨGA codes for tyrosine and phenylalanine, thus suggesting a new mode of decoding. Our results also suggest that RNA modification, as a naturally occurring mechanism, may offer a new way to expand the genetic code.
Comment in
Nature. 2011 Jun 16;474(7351):289-90.
PMID: 21677757

This is pretty cool, but it sounds like it's still a long way from implementation in humans.

[niner]

http://www.dailymail...s.html?ITO=1490

That's my Lab. I work at the Gorbunova lab. Lemme know if you have any questions (I'm an undergrad there, so I can direct questions to the researchers as well)

That's awesome! We have people in high places! So how do they get the little H/ACA RNA expressed in the cell? That sounds like full-blown genetic engineering...

[Elus]

http://www.dailymail...s.html?ITO=1490

That's my Lab. I work at the Gorbunova lab. Lemme know if you have any questions (I'm an undergrad there, so I can direct questions to the researchers as well)

That's awesome! We have people in high places! So how do they get the little H/ACA RNA expressed in the cell? That sounds like full-blown genetic engineering...

This is the paper the OP was talking about :

http://www.sciencema...t/332/6036/1443

[niner]

http://www.dailymail...s.html?ITO=1490

That's my Lab. I work at the Gorbunova lab. Lemme know if you have any questions (I'm an undergrad there, so I can direct questions to the researchers as well)

That's awesome! We have people in high places! So how do they get the little H/ACA RNA expressed in the cell? That sounds like full-blown genetic engineering...

This is the paper the OP was talking about :

http://www.sciencema...t/332/6036/1443

Oh, oops. I thought that paper got included by mistake because it was also from Rochester! The other links were about a different paper- Karijolich & Yu. That was the one that I was interested in.

[ihatesnow]

http://www.dailymail...s.html?ITO=1490

That's my Lab. I work at the Gorbunova lab. Lemme know if you have any questions (I'm an undergrad there, so I can direct questions to the researchers as well)

im from the rochester area .....best of luck to you

[ihatesnow]

http://www.urmc.roch...dex.cfm?id=3226