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LongeCity .                       Advocacy & Research for Unlimited Lifespans

Microglia Stem Cells



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Transplanted microglia - where did they go?

Posted by Mondey , 10 May 2017 - - - - - - · 1,296 views
microglia therapy
Hi,
yes it has been a while since I wrote something here.  Here are two papers pusblished including the work funded by you:
Leovsky C, Fabian C, Naaldijk Y, Jäger C, Jang HJ, Böhme J, Rudolph L, Stolzing A. Biodistribution of in vitro-derived microglia applied intranasally and intravenously to mice: effects of aging. Cytotherapy. 2015 Nov;17(11):1617...


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Amyloid load

Posted by Mondey , 08 October 2012 - * * * * * · 1,424 views

Amyloid load As for the effect the transplanted microglia have on the pathology: I do not see a big difference in the amount of amyloid in the Alzheimer mouse brain without cells (A) and with microglia transplanted (B). So we have to wait until we get all brains analysed by sterology for the exact amyloid quantification.

Greetings,
Dey


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Some transplantations done - more to come

Posted by Mondey , 08 October 2012 - - - - - - · 934 views

Some transplantations done  - more to come Hi again,
so we have started with our transplantations and some of our animals have been analysed. You see a picture showing our transplanted microglia in green and the amyloid in red. You can notice that the microglia are around and in the vessels. We also know from our PCR data that we get quite some microglia into the brain. This data was gathered 4...


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Brain histology - microglia

Posted by Mondey , 20 May 2012 - * * * * * · 3,590 views

Brain histology - microglia To visualize microglia and amyloid plaques in vivo, we established different staining protocols (including histology and immunohistology) to later evaluate microglia number after transplantation and also amyloid load. Top left is showing an immuno staining for amyloid and top right is an overview for microglia in the...


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Charaterisation of microglia

Posted by Mondey , 19 May 2012 - - - - - - · 1,555 views

Charaterisation of microglia We used several markers to prove that what looks like microglia really are microglia. We used so called surface markers (CDs) including CD11b and CD45. These markers are also used to distinguish between what people all macrophages and what people think microglia are (there are a lot of discussions about the identity of microglia). The cells in the red...


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Selfmade microglia

Posted by Mondey , 19 May 2012 - - - - - - · 1,610 views

Selfmade microglia To start with the microglia replacement therapy we need lots of cells and they must resemble the original microglia. In the pictures you see microglia isolated from mice (left) or self-made microglia derived from bone marrow (in vitro differentiated).
In both cases they have basically the same morphology. In case of the self-made microglia we only have more...


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Background

Posted by Mondey , 18 May 2012 - - - - - - · 1,626 views
microglia
Background As a reminder WHY we are doing this:

What are microglia:

Microglia comprise about 10% of the cell population of the brain and form the main first immune defense of the CNS. They are phagocytic, cytotoxic, present antigens and they can promote repair after injury (Simard 2007).  
Microglia in the brain display normally a quiescent state in which...


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Microglia Stem Cells: Community update

Posted by Mondey , 18 May 2012 - - - - - - · 1,140 views
microglia, stem cells, therapy and 1 more...
Microglia Stem Cells: Community update In 2011, the Longecity community raised money to support a research project on microglia stem cell therapy.  Over the next few days, we will be reporting interim results in this blog.





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