4019 replies to this topic

[BigPapaChakra]

 

 

- Full blown cold adaptation

- CryoHelmet

 

 

I bet BPC, is mainly hacking his wife's sleep as well as his own :-)

 

Haha, fortunately our daughter sleeps through the night, so we're blessed/lucky in that sense. I am doing some sleep hacking though!

 

 

glad to hear about the daughter :-). My son still doesn't sleep through the night, but it's not much of a hassle for us with all our biohacking.

 

On your cold adaptation. I am doing the Wim Hof Method thing and so far so good. It's actually quite fun. Not at the part of the icebaths yet (10 minute cold showers is where I am at).

 

Can you tell a bit more about the cryohelmet?

 

the latest fad in sf is apparently http://www.cryohealthcare.com/. Got this through a Tim Ferris podcast.

 

Haha, you guys have been doing quite a lot of hacking. Still doing the intranasal LLLT and PQQ and all that? How old is your son now if you don't mind me asking?

 

This was the cryohelmet I was speaking about: http://iceyourhead.com/cryohelmet/I know of 3 people on facebook now that are using it (four if including one guy's wife) and all have spoken highly of it. The literature is there to support its use, as well. I'm going to get that, the Cool Fat Burner, and the Cool Gut Buster soon. Thinking about using a snorkel or something and dunking my entire head in ice water as well for the mammalian dive reflex, but holding my breath as opposed to 'hacking' it with a snorkel may provide better effects.

 

I'll post an interesting review of 'hydrotherapy' later, which really I just used for nice citations on ice water immersion + CO2 (kinda like combining the diametrically opposed Jack Kruse and Dr. Peat, lol). 

[lostfalco]

For Acquisition: take an acetylcholinesterase inhibitor 30 min before studying. 

For Consolidation: take a phosphodiesterase 5 inhibitor right after studying.  

Disclaimer: if you're a rodent learning object information. =)

 

I just started testing out sildenafil/Viagra in place of tadalafil/Cialis. We'll see how it compares. I promise to get back to ICES soon! So many experiments, so little time.

 

http://www.pubmed.com/pubmed/15630588

 

Psychopharmacology (Berl). 2005 Feb;177(4):381-90. Epub 2004 Jul 24.

Dissociable effects of acetylcholinesterase inhibitors and phosphodiesterase type 5 inhibitors on object recognition memory: acquisition versus consolidation.

Prickaerts J1, Sik A, van der Staay FJ, de Vente J, Blokland A.

Author information

Abstract

RATIONALE: 

Phosphodiesterase enzyme type 5 (PDE5) inhibitors and acetylcholinesterase (AChE) inhibitors have cognition-enhancing properties. However, it is not known whether these drug classes affect the same memory processes.

OBJECTIVE: 

We investigated the memory-enhancing effects of the PDE5 inhibitor sildenafil and AChE inhibitors metrifonate and donepezil in the object recognition task to find out whether acquisition or consolidation processes were affected by these drugs.

METHODS: 

The object recognition task measures whether rats remembered an object they have explored in a previous learning trial. All drugs were given orally 30 min before or immediately after learning to study acquisition and consolidation, respectively.

RESULTS: 

Sildenafil given immediately after the first trial improved the memory performance after 24 h and resulted in an inverted U-shaped dose-effect curve with the peak dose at 3 mg/kg. When given before the first trial, sildenafil also improved the memory performance. However, the dose needed for the best performance under this condition was 10 mg/kg, suggesting that the dose-effect curve shifted to the right. This can be explained by the metabolic clearance of the high dose of sildenafil. Donepezil had no memory improving effect when given after the first trial. However, when given before the first trial, a gradually increasing dose-effect curve was found which had its maximum effect at the highest dose tested (1 mg/kg). Likewise, only when metrifonate (30 mg/kg) was given before the first trial did rats show an improved memory performance.

CONCLUSION: 

Our data strongly suggest that PDE5 inhibitors improve processes of consolidation of object information, whereas AChE inhibitors improve processes of acquisition of object information.

 

Edited by lostfalco, 11 May 2015 - 04:50 PM.

[BieraK]

Hello to all
I was doing some questions to the Dr. Gonzalez-Lima about Methylene Blue and LLLT
That was initiated by this study that reviews the use of mitochondrial enhancers for cognitive enhancement and protection: http://www.sciencedi...06295213007417 
The study addresses the use of Methylene Blue, LLLT and Ketogenic Diet. Ketogenic Diet combined with Methylene Blue or LLLT does not show problems, the question in this thread was always concerned to the combination of Methylene Blue and LLLT

Dr. Gonzalez-lima allowed me to share the some of the information that he gave me in conversation via email

Well I asked to Dr. Gonzalez-lima if is safe or dangerous to combine LLLT and MB, because the potential phototoxicity effects of MB an he reply the next:
 

 

The research takes into account only the use of one intervention at the same time because I am not aware of any combinations reported so far. But in principle they could be combined. The MB phototoxicity does not apply to near-infrared light as used for LLLT, and ketogenesis is also compatible with MB and LLLT.

I hope this helps. Thank you for your interest.

 

and then in a next email:

 

There is nothing to be concerned about low-dose MB with near-infrared light for transcranial LLLT. This is a concern apparently raised by some people that do not understand what they are talking about. In my previous reply I stated "The MB phototoxicity does not apply tonear-infrared light as used for LLLT"

Methylene blue is a "blue" dye when oxidized. Like all blue dyes, this means that by definition MB reflects light in the blue part of the visible light spectrum and absorbs light in the red part. And red light has much lower tissue penetration than near-infrared, so I recommend only near-infrared or infrared light for transcranial human brain studies of LLLT.  I used infrared (1064 nm) in my human studies:
http://journal.front...2014.00036/full

 

This is a very interesting information, it is important to thank to him for his good availability and for agreeing to share the information, I hope this could clear some doubts about this two interventions.

Edited by BieraK, 12 May 2015 - 03:12 AM.

[Q did it!]

Thanks for clearing up the MB and LLLT/TULIP combination issue/concern. Been wanting to try MB but not wanting to quit or cycle TUILP. This is deff good news! Hopefully myself and others will try this combo to find if it yields much nootropic value

[BieraK]

Yes... I asked to him other things. There is important to note that for the use of LLLT while MB is already in the body the light needs to be near infrared or infrared. 

 

1) All the last explanation was clear, near infrared or infrared ligh is safe with the use of MB, but concerning to red light? Is the safety applicable here?... 
Reply: I do not know the answer but to be safe I only recommend low-dose MB together with near-infrared or infrared light.

So for LOW DOSE Methylene blue, the safe side is near infrared or infra red.

I've done sessions with 660 nm (red light), but with the application of LLLT first and then with the application of MB. 

Other important thing is the MB dose, in the study of Dr Gonzalez-lima are stated the range of the low dose methylene blue:
http://www.sciencedi...006295213007417

Cited from the study:

 

MB shows a hormetic dose–response, with opposite effects at low and high doses [32]. At low doses of 0.5–4 mg/kg, MB is an electron cycler in the mitochondrial electron transport chain, with unparalleled antioxidant and cell respiration-enhancing properties that affect neural function in a versatile manner. The unique auto-oxidizing property of MB and its pleiotropic effects on a number of tissue oxidases explain its potent neuroprotective and metabolic effects at low doses. A major role of the mitochondrial respiratory enzyme cytochrome oxidase on the neuroprotective and cognitiveenhancing effects of MB is supported by available data [26].

LOW DOSE USP MB: 0,5 - 4 mg/kg

 

Here's another important thing he clarified to me:
Cited again from the same study

 

Low-dose MB does not affect the nitric oxide-guanylyl cyclase system as high-dose MB, but low-dose MB’s auto-oxidizing property acts as an electron cycler that allows MB to redirect electrons to the mitochondrial electron transport chain, thereby enhancing ATP production and promoting cell survival [26]. In bypassing complex I–III activity to generate ATP, low-dose MB reduces reactive oxygen species (ROS) production from the mitochondrial electron transport chain, which has the potential to minimize AD physiopathology [5].

Low dose MB does not inhibit Nitric Oxide, compatible with PDE5 inhibitors

And finally

 

What happens with the interaction of antioxidants and LLLT... I was thinking in the interaction of N-Acetyl Cysteine with LLLT, Could antioxidant intake be detrimental for the effects of LLLT?

Reply: I do not think so because LLLT-induced cytochrome oxidase effects increase energy production. So antioxidant actions should not be detrimental. 

Taking antioxidants like NAC do not interfere with the effects  

We need more people doing academic research in this forum  ... doing studies about all this things jojojo 

Edited by BieraK, 12 May 2015 - 06:58 AM.

[BieraK]

Thanks for clearing up the MB and LLLT/TULIP combination issue/concern. Been wanting to try MB but not wanting to quit or cycle TUILP. This is deff good news! Hopefully myself and others will try this combo to find if it yields much nootropic value

 I agree with you, please if you try the combo report your experience.

The other interesting thing, if antioxidants like NAC or if the antioxidants properties of MB does not block the LLLT effects, PQQ taken before LLLT is a good option. 
 

[mettmett]

Good stuff bierak. I'm going to start a pqq/mb/lllt combo right away

[De La Torre]

Good stuff bierak. I'm going to start a pqq/mb/lllt combo right away

What is the recommended dosage, how is it taken, and what potential contraindications are there?

Edited by De La Torre, 14 May 2015 - 01:09 AM.

[mettmett]

I will be taking 10mg pqq daily (to save money), I'm experimenting with my optimal dose of MB per Joseph Cohen's recommendations at selfhacked (http://selfhacked.co...itive-enhancer/), and the standard every other day with the laser.

So mornings = pqq, then laser, then mb is how I'm doing it.

Well if what bierak posted is true I don't see many negative interactions if any.

Typing hastily on my phone, sorry for any misspellings

[De La Torre]

So, you're basically just using MB as a substitute for coq10/ubiquinol?

[BieraK]

Please try to read the studies done by Dr Gonzalez-Lima
http://www.sciencedi...006295213007417

In this study is stated what is considered low dose Methylene Blue, that is  0,5 - 4 mg/kg for a human.
Lower dosages work  but with less potency, I don't know why dosages in the mcg range, like 60 mcg was presented as the proper dosage for mitochondrial enhancement.

I will quote another part of the study:
 

Whether a beneficial use of low-dose MB in aging populations may be generalized to treat MCI and early AD patients should be investigated. For example, a research group garnered a great deal of media attention after a 2008 Alzheimer’s Association international conference where they presented preliminary data using MB in a study showing that low-dose MB prevented cognitive deterioration in AD patients. Using the trade name of RemberTM, and the MB synonym methylthioninium chloride, MB was given orally at 60 mg three times a day (a low dose of 2.57 mg/kg/day based on a body weight of 70 kg) for 24 months to patients with mild to moderate AD [64]. There was an 81% reduction in the rate of cognitive decline compared to controls after 50 weeks. This is an effect that, if reproducible, would be without precedent in pharmacological interventions against AD. But data from this abstract and meeting presentation should be regarded as preliminary since it has not been published after a peer-reviewed process. The authors suggested that the cognitive benefit of MB on AD patients was linked to prevention of Tau aggregation, based on prior in vitro studies with high-dose MB [65]. Since MB improves cognition only at low doses, while it prevents while it prevents Tau aggregation only at high doses, it is more likely that low-dose MB is acting via mitochondrial respiration mechanisms for neuroprotection and cognitive enhancement, as proposed here (Fig. 1).

 

 

I will be taking 10mg pqq daily (to save money), I'm experimenting with my optimal dose of MB per Joseph Cohen's recommendations at selfhacked (http://selfhacked.co...itive-enhancer/), and the standard every other day with the laser.

So mornings = pqq, then laser, then mb is how I'm doing it.

Well if what bierak posted is true I don't see many negative interactions if any.

Typing hastily on my phone, sorry for any misspellings

 

If you are using MB after the application of LLLT I don't see any problem using the dosage of 0.5 - 4 mg/kg. 60 mcg is very low, however in my first application of LLLT+MB I've used 160 mcg after the application of LLLT, I worked, but as today I don't feel any thing with that low dosage.

I've not used 20 mg of MB yet (o.5 - 4 mg/kg range), I'm hoping that methylene blue powder comes from the UK.

I've attached a picture of the conversation to prove that all this information is not my invention, I hope that Dr. Gonzalez-lima do not bother about this

Attached Files

•  picture about the truth.JPG   52.79KB   6 downloads

Edited by BieraK, 14 May 2015 - 04:31 AM.

[BieraK]

 

Good stuff bierak. I'm going to start a pqq/mb/lllt combo right away

What is the recommended dosage, how is it taken, and what potential contraindications are there?

 

 

Mitochondrial respiration as a target for neuroprotection and cognitive enhancement
http://www.sciencedi...006295213007417

MB shows a hormetic dose–response, with opposite effects at low and high doses [32]. At low doses of 0.5–4 mg/kg, MB is an electron cycler in the mitochondrial electron transport chain, with unparalleled antioxidant and cell respiration-enhancing properties that affect neural function in a versatile manner

 

Edited by BieraK, 14 May 2015 - 04:34 AM.

[mettmett]

So, you're basically just using MB as a substitute for coq10/ubiquinol?

Well it's more because pqq is the only thing I have currently

[BieraK]

Low dose methylene blue: 0.5 - 4 mg/kg

For a 60 kg person: 30 mg - 240 mg
For a 70 kg person: 35 mg - 280 mg

http://www.sciencedi...006295213007417
Using the trade name of RemberTM, and the MB synonym methylthioninium chloride, MB was given orally at 60 mg three times a day (a low dose of 2.57 mg/kg/day based on a body weight of 70 kg) for 24 months to patients with mild to moderate AD [64]

 

Edited by BieraK, 14 May 2015 - 09:57 PM.

[lostfalco]

 

I was doing some questions to the Dr. Gonzalez-Lima about Methylene Blue and LLLT

BieraK! Awesome work, man. Thanks for checking with Dr. Gonzalez-Lima, getting his insights, and reporting back. 

 

I'm with you on the dosing. When I ran a methylene blue self experiment a couple of months ago I dosed in the mg range. I bought mine off of Niles on ebay. http://www.ebay.com/...e-/171140878350

 

Apparently, there is also another user here on Longecity who sells 99.8% pure MB. http://www.longecity...s/#entry727834 

 

Let me just warn you that your urine will be bright blue at this dose and MB stains everything! I also ran into some gastrointestinal issues with it but I'm willing to give it another go now that school is over. 

 

Since a number of us are going to be testing this I checked with /u/MisterYouAreSoDumb over on reddit and he indicated that Ceretropic would be interested in creating a MB product. He wanted to get some insights from us on the dose that we find to be the most effective so if everyone who tests this out could report back we might be able to determine a reasonably optimal range. Pretty damn cool that we might be able to contribute to the creation of a Ceretropic product!

 

Anyway, I just ordered some MB from here and I'll see how it compares to Niles' batch. https://www.bluebrai...ylene-blue-usp/

 

Thanks again for getting the ball rolling on this BieraK. I'm looking forward to seeing how this combines with LLLT. 

Edited by lostfalco, 17 May 2015 - 04:01 PM.

[mettmett]

I'll update with the study later, but I was disappointed to find out that MB inhibits nitric oxide production

[lostfalco]

I'll update with the study later, but I was disappointed to find out that MB inhibits nitric oxide production

This one? http://www.ncbi.nlm....les/PMC3770994/

 

It's a review article but it goes into a fair amount of detail on the relationship between MB and NO. 

 

"Most recently, methylene blue has been used as a treatment for refractory distributive shock through a lesser known property: inhibition of the downstream effect of nitric oxide (NO)."

 

"There is experimental and clinical evidence that support the use of a selective NO–cGMP pathway inhibitor, methylene blue, as a treatment in cases of refractory vasodilatory shock with no response to conventional treatment."

 

"Plasma nitrate, a marker of NO release, did not differ between groups. This finding suggests that methylene blue does not mediate its action through NOS inhibition or NO scavenging, and suggests that its primary action may be mediated through GC inhibition [56]."

 

"The dosing regimen for methylene blue based on experimental and clinical data is not entirely clear but does seem to be similar to what is used for the treatment of methemoglobinemia: 1–2 mg/kg."

 

"It is clear based on other studies that the use of high doses of methylene blue, typically doses greater than 7 mg/kg, is associated with adverse effects such as paradoxical induction of methemoglobinemia, acute hemolytic anemia, and detrimental effects on pulmonary function [86, 87]."

 

"Methylene blue may cause serotonin syndrome because both the parent compound and metabolite, azure B, inhibit MAO-A [92]. Methylene blue causing serotonin syndrome is relatively rare occurring mostly with the use of very high doses."

Edited by lostfalco, 17 May 2015 - 04:15 PM.

[mettmett]

It was actually this one: http://www.ncbi.nlm..../pubmed/7679577

"Our data suggest that methylene blue acts as a direct inhibitor of NO synthase"

What I'm confused about is this:
Your study says
"Plasma nitrate, a marker of NO release, did not differ between groups. This finding suggests that methylene blue does not mediate its action through NOS inhibition"

Ok well the NOS pathway doesn't use nitrates to produce NO, it uses arginine.

It is the nitrate->nitrite->nitric oxide pathway that uses Nitrates.

Therefore NOS inhibition wouldn't mess with nitrate levels anyway....

So let's say MB only inhibits the NOS pathway but leaves the Nitrate pathway untouched.

Well the research is still saying that MB inhibits the cGMP that NO increases, and It is this increased cGMP that makes NO so beneficial.

In other words it doesn't matter if MB inhibits both pathways or not because it still interferes with the beneficial downstream effects.

I just received berkelys nitric oxide test strips in the mail and I tested very low for NO as I suspected.

So considering these new findings I won't be moving forward with MB and will instead be focusing on hacking NO to reach optimal levels.

[BieraK]

http://www.sciencedi...006295213007417
 

Low-dose MB does not affect the nitric oxide-guanylyl cyclase system as high-dose MB, but low-dose MB’s auto-oxidizing property acts as an electron cycler that allows MB to redirect electrons to the mitochondrial electron transport chain, thereby enhancing ATP production and promoting cell survival [26]. In bypassing complex I–III activity to generate ATP, low-dose MB reduces reactive oxygen species (ROS) production from the mitochondrial electron transport chain, which has the potential to minimize AD physiopathology [5].

 

[mettmett]

Well that's reassuring. And I'm assuming that 60MG is considered a low dose?

Would this also mean that the Mao inhibition and serotonin syndrome aren't issues at low doses?

I really need to buy that study for full access -_-

Edited by mettmett, 17 May 2015 - 11:24 PM.

[Q did it!]

A quick question, Would something like this  with the hydrogen water be a swell idea? I know it hasn't been talked about much for a while. And will be testing MB+TUlIP soon

[APBT]

FULL TEXT:

 

 

Attached Files

•  Mitochondrial respiration as a target for neuroprotection and cognitive enhancement.pdf   512.52KB   22 downloads

[middpanther88]

So, if anything, what would one expect the hydrogen water to do?  Just wondering.

[BieraK]

I've a question, perhaps Lostfalco could answer this

In what way I can use the LED only in my hair follicles? 
I wan't to use the LED in brain just one time in the week, but in the other days I wan't to use this in my hair follicles for alopecia. What I need to do? Perhaps use the LED in a longer distance?

I don't want to use the LED always in my brain for two reasons:
1) I want to stimulate just certain areas and not all the brain:
I wan't to stimulate only Wernicke, Broca, Left Dorsolateral pre frontal cortex, Right posterior parietal cortex and right anterior temporal lobe but alopecia has it effects in more areas.

2) I don't wan't to generate an hyperconnected brain

Edited by BieraK, 22 May 2015 - 04:50 AM.

[lostfalco]

I've a question, perhaps Lostfalco could answer this

In what way I can use the LED only in my hair follicles? 
I wan't to use the LED in brain just one time in the week, but in the other days I wan't to use this in my hair follicles for alopecia. What I need to do? Perhaps use the LED in a longer distance?

I don't want to use the LED always in my brain for two reasons:
1) I want to stimulate just certain areas and not all the brain:
I wan't to stimulate only Wernicke, Broca, Left Dorsolateral pre frontal cortex, Right posterior parietal cortex and right anterior temporal lobe but alopecia has it effects in more areas.

2) I don't wan't to generate an hyperconnected brain

Hey, BieraK. The primary wavelength that has been tested for alopecia is 650nm. It is currently unknown if the 810nm to 850nm range will work for hair loss. What is the wavelength of the LEDs that you have?

 

http://www.ncbi.nlm....es/PMC3944668/ 

 

"Most studies investigating effects of LLLT on hair growth have used wavelengths that range from 635 to 650 nm, but as of today no study has compared the effect of near-infrared wavelengths such as 810 nm, which have deeper penetrating capacities, to red light."

[lostfalco]

So, if anything, what would one expect the hydrogen water to do?  Just wondering.

Hey middpanther88, here's a very thorough compilation of many of the studies. http://www.molecular...te.com/studies/

 

LeBaron also has some decent articles here as well. =)   http://www.molecular...re-information/

[lostfalco]

A quick question...

Hey, what's up Q? I've enjoyed your YouTube videos btw. Thanks for getting the word out on some of the stacks we've been experimenting with here on Longecity. Here are his two TULIP videos if anyone is interested. =)  

 

 

 

As far as vortexing water goes...I'm not totally sure one way or the other but Dr. Pollack seems to think it's a good idea. Speaking of which.... 

 

Joe just posted his recent interview with our old friend Dr. Gerald Pollack and I definitely recommend checking it out to everyone who has the time. 

 

http://selfhacked.co...optimal-health/

 

Edited by lostfalco, 22 May 2015 - 10:16 PM.

[BieraK]

 

I've a question, perhaps Lostfalco could answer this

In what way I can use the LED only in my hair follicles? 
I wan't to use the LED in brain just one time in the week, but in the other days I wan't to use this in my hair follicles for alopecia. What I need to do? Perhaps use the LED in a longer distance?

I don't want to use the LED always in my brain for two reasons:
1) I want to stimulate just certain areas and not all the brain:
I wan't to stimulate only Wernicke, Broca, Left Dorsolateral pre frontal cortex, Right posterior parietal cortex and right anterior temporal lobe but alopecia has it effects in more areas.

2) I don't wan't to generate an hyperconnected brain

Hey, BieraK. The primary wavelength that has been tested for alopecia is 650nm. It is currently unknown if the 810nm to 850nm range will work for hair loss. What is the wavelength of the LEDs that you have?

 

http://www.ncbi.nlm....es/PMC3944668/ 

 

"Most studies investigating effects of LLLT on hair growth have used wavelengths that range from 635 to 650 nm, but as of today no study has compared the effect of near-infrared wavelengths such as 810 nm, which have deeper penetrating capacities, to red light."

 

Hello
My LED is of 850 nm.... I've a laser of 650 nm, however I do no use that very much because is waste much time, the laser has a tinny point.

I can't see why a 850nm laser can't work for alopecia... however the empirical contrastation is needed.
For now I wan't how to only stimulate my hair and not to stimulate my brain... because I don't wan't to do only a one session of LLLT in the week, the reasons are that I don't what to stimulate all the brain areas, but I wan't to stimulate all the scalp area :P

[Jochen]

Falco, apologise if this was already mentioned, but I stumbled upon this while reading Norman Doidge new book: "The Brain's way of healing". It has a whole chapter (and more dedicated to LLT).

 

Apparently one of the persons that has really delved into this in the west is Dr Fred Khan.  (80+ years old btw)

He really has a lot of case material and has a clinic dedicated to LLT treatments of his patients.

http://www.bioflexlaser.com/

 

He developed a customisable laser system (you can program the wavelength) and it looks pretty nifty from the studies I have now read.

 

this is his blog: http://drkahnblog.bioflexlaser.com/

 

Need to read through a lot more before I can synthesize a bit :-)

[lostfalco]

Falco, apologise if this was already mentioned, but I stumbled upon this while reading Norman Doidge new book: "The Brain's way of healing". It has a whole chapter (and more dedicated to LLT).

 

Apparently one of the persons that has really delved into this in the west is Dr Fred Khan.

Thanks, Jochen. I'll have to check out Doidge's new book. I read 'The Brain that Changes Itself' awhile back and thought it had some pretty interesting stuff. 

 

Dr. Khan's website looked interesting as well. I especially liked the blog entry from April 13th, 2015 which explains why more power is not better with LLLT, the role of wavelength, and why contact and pressure are important. Thanks for the link! 

 

"With contact, more light it forced into the tissue. And by using pressure, blood, being the main absorber of the light, is reduced in the area and the light can more easily penetrate the ischemic tissue."