4 replies to this topic

[pi-]

Trichostatin A is an HDAC inhibitor.

 

http://www.wikidoc.o.../Trichostatin_A says:

 

Trichostatin A is an organic compound that serves as an antifungal antibiotic and selectively inhibits the mammalian histone deacetylase family of enzymes. TSA inhibits the eukaryotic cell cycle during the beginning of the growth stage. TSA can be used to alter gene expression by interfering with the removal of acetyl groups from histones and therefore altering the ability of DNA transcription factors to access the DNA molecules inside chromatin. Thus, TSA has some uses as an anti-cancer drug. By promoting the expression of apoptosis-related genes, it may lead to cancerous cells surviving at lower rates, thus slowing the progression of cancer.

 

I've read a paper (http://www.ncbi.nlm....les/PMC3848041/) demonstrating that a weak HDAC inhibitor (valproate) promotes plasticity.

 

Wikipedia lists 5 sets of HDAC inhibitors (http://en.wikipedia...._classification) in order of decreasing effectiveness.  Valproate is at the bottom.  Trichostatin A is at the top!

 

I made a separate post about HDAC inhibitors: http://www.longecity...dac-inhibitors/ but I am now looking at Trichostatin A.

 

I can't figure out whether it is harmful or safe.

• A safety sheet (https://www.caymanch...s/10006391m.pdf) doesn't suggest anything more dangerous than a skin/eye irritant.
• A pubmed report says it kills cancer cells (http://www.ncbi.nlm....les/PMC2984103/).
• In fact I can't see a bad word about it in PubMed http://www.ncbi.nlm....D loprovpmc[sb] (although I just skimmed the first page)

 

So, what's the catch?  COST!  It seems to cost $80/mg, with a typical dose being ~50mg ($4000).

http://anabolicminds...n-mystatin.html

https://www.molport....OGQSA-N/3959819

 

meh.  That is a little out of my budget range.

 

Is it only so expensive because it is produced in trace quantities because there is no demand for it? Or is there something inherently difficult in its synthesis?

 

i.e. Is there any chance of persuading some lab in China to make a large enough quantity that the price comes down to something affordable like $5/dose?

Edited by pi-, 12 July 2014 - 10:33 AM.

[Nattzor]

The price is most likely based on sigma aldrich or the like, they supply very pure stuff to universities/companies and charge a fortune, I'd guess the price is much lower for a RC seller.

[pi-]

I'm just going to call it TSA from now on.

 

I've just searched through longecity for TSA; here are the interesting posts:

 

http://www.longecity...e-2#entry583228

Erebus suggests TSA as a good candidate for HDACi, and valproate bad

 

http://www.longecity...nd/#entry666450

http://www.longecity...-aging-protein/

http://www.longecity...er/#entry662013

^ LexLex seems to have posted a few times on TSA

 

http://www.longecity...cs/#entry550525

^ interesting quote, HDACi-s proving useful fighting degenerative brain diseases

 

http://www.longecity...ic-experiments/

^ LostFalco mentions it in a supremely interesting post / thread

 

http://www.longecity...-67#entry662273

^ phoenicis seems to have found 'tributyrin' acts similarly to TSA at a fraction of the cost

also that post references http://www.ncbi.nlm....pubmed/22771460

 

http://www.longecity...kled-psoriasis/

^ HDACi seems to have some bearing on psoriasis, phoenicis again

 

http://www.longecity...15-follistatin/

^ TSA seems to have some positive effect on muscle growth, I think I've seen it mentioned in bodybuilding forums also

 

http://www.longecity...trichostatin-a/

^ TSA has a thread in the supplements forum

 

it seems to be frequently mentioned as anti-cancer and anti-ageing.

[joelcairo]

Using a high-powered HDAC inhibitor is a bit like hitting your DNA with a hammer. Some healthful genes that gradually become diminished with age may be re-expressed, but there is also the possibility for genes that are supposed to be repressed to become active, including tumor promoter genes. TSA is a very exciting anticancer drug, but if you don't have cancer I'm not sure I'd mess with it. OTOH, valproate is cheap and has mild side effects and the effects of longterm use are known.

 

Note that more and more polyphenols are being discovered to have HDAC inhibitory effects and/or gene demethylating effects. The major ones like curcumin and EGCG and sulforaphane and genistein of course. I'm certain the list will grow much longer as more substances are tested for this effect.

Edited by joelcairo, 12 July 2014 - 07:28 PM.

[gintrux]

As I'm getting interested in HDAC inhibitors the more I look into them, bumping this thread, have you managed to obtain and try it?