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DNA Methylation Analysis

osiris green aging biomarkers dna methylation

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#1 Mind

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Posted 23 January 2017 - 10:57 PM


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#2 Mind

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Posted 23 January 2017 - 11:05 PM

For Immortality Institute  Members:

Osiris Green is offering a 30% discount. You can find the discount code here: http://www.longecity...ember-discount/

 

 

Attached File  osirisgreen2.png   2.12KB   0 downloads


Edited by caliban, 24 January 2017 - 02:13 AM.
name & pic


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#3 neilcopes

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Posted 24 January 2017 - 10:04 PM

Hi, I’m Dr. Neil Copes, CEO of Osiris Green.  The service that we’re offering is a mail-in saliva testing service that can give an estimate of your chronological age based on observed epigenetic changes in your DNA.  I am very interested in the life extension community and I’m hoping that this service might be as a useful tool for people who want to monitor their age from an epigenetic standpoint.  The FAQ page on our website is a useful place to start if you’re interested.  Also, I recently gave an interview at FightAging.org that might provide some additional background on what we’re trying to do.
 
Feel free to ask me any questions, and I’ll replay as time permits.


#4 Tom Andre F. (ex shinobi)

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Posted 29 January 2017 - 05:52 PM

Hi,

 

can you please explain deeper this topic please ? for instance, whats the difference between DNA methylation and mtDNA methylation please though aging ?

 

Is it more reliable than the telomeres lenght test ? your opinion please

 

And status of γH2AX ? wich is used as very reliable to give DNA damage status

 

Is saliva reliable to know what state are generally our others cells ?

 

And finally is it possible to directly reverse this clock biomarker ?

 

thank you !



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#5 neilcopes

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Posted 13 February 2017 - 03:57 PM

Hi.  Thank you for the questions, and I apologize for the EXTREMELY long delay in responding (a combination of being out of town, along with a wicked head cold).  
 
The main difference between DNA methylation and mtDNA methylation, as far as I’m aware, is that mtDNA methylation has been much less studied.  Likely due to its low levels and higher degree of variability, mtDNA methylation wasn’t fully confirmed by the scientific community until the early 2000s.  As far as age-related methylation is concerned, I know that several mtDNA methylation sites have shown a linear correlation to chronological age (Mawlood SK, et al. 2016), but the coefficient of correlation (the tightness of the scatter in the data) was reported as lower than in other methods.
 
As far as I’m aware, epigenetic methods of chronological age determination are more accurate than telomere length measurements.  Telomere length is affected by multiple factors, including oxidative stress.  (Global DNA methylation too is affected by a huge number of environmental factors, but multiple specific DNA regions seem to be more affected by chronological age than anything else.)  As for y-H2AX, as you stated it is a very reliable marker for double-strand breaks in DNA, and the level of y-H2AX expression has been shown to increase with age until around the age of 57, when it’s increase plateaus (Schurman SH, et al. 2012).  I have not seen y-H2AX levels used as a predictor of chronological age, although I suppose it potentially could be for individuals below 60 years old. 
 
The method I’m using relies on DNA isolated from buccal cells (epithelial cells of the cheek and gums).  From my personal experience, these are more than adequate for purposes of DNA methylation analysis for estimating chronological age.  I can’t state as to how these cells in general compare to other cell types within the body, but if I had to make an educated guess I would say that they are probably fairly similar in physiology and gene expression to other epithelial cells.
 
As to your last question: is it possible to reverse this clock biomarker?  I certainly hope so.  My assumption is that most age-related DNA methylation changes are the result of random epigenetic drift (hypermethylation) and inadequate recovery of epigenetic state after DNA repair (hypomethylation).  Some of these age-related epigenetic changes though likely reflect gene regulation that is part of the general developmental inertia that occurs past puberty (there’s an excellent book by Dr. Richard Walker that discusses this topic – he doesn’t get enough attention in my opinion) as well as cellular changes happening in response to body-wide factors such as senescent cell accumulation and immune system decline.  Likely, any effective antiaging therapy would at least slow those epigenetic changes, if not reverse them entirely.

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