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Cycling Protocols for NAD+ Precursors

nad+ nmn cycling supplements sulforaphane sublingual bioavailability tolerance downregulation

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#61 LawrenceW

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Posted 24 November 2019 - 12:10 AM

And a very speedy response countering Chromadex's Dr. Brenner:

 

"We appreciate the authors’ insightful comments and the opportunity to clarify the important issues related to our recent publication2. However, their claim of ‘absence of evidence’ has no concrete foundation and the data in our article strongly support our conclusion that Slc12a8 is an NMN transporter, although there are still many interesting questions regarding the structure and function of Slc12a8 as an NMN transporter."

 

https://www.nature.c...DUOdTkcoS9pdnYo


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#62 MikeDC

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Posted 24 November 2019 - 02:46 AM

https://www.nature.c...2255-019-0085-0

The new Fig. 2 in the response by Grozio et al. makes matters worse because they did not analyse what an NMN standard looks like in the hepatocyte extract and decided to quantify a peak that has inexplicably drifted from a 22-min retention time to a 19-min retention time.
Previous work on FK866-resistant NMN incorporation showed quantitative dependence on nicotinamide riboside kinase 1, quantitative extracellular conversion of NMN to nicotinamide riboside and intracellular detection of nicotinamide riboside before formation of intracellular NMN1. Moreover, it has been demonstrated that modification of the phosphate in extracellularly applied NMN is required to activate the widely expressed sterile alpha and TIR motif-containing protein 1 (SARM1) to induce an NMN-dependent cell death pathway because NMN itself is not transported7.

Edited by MikeDC, 24 November 2019 - 03:05 AM.

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#63 able

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Posted 14 June 2020 - 09:55 PM

https://www.nature.c...2255-019-0085-0

The new Fig. 2 in the response by Grozio et al. makes matters worse because they did not analyse what an NMN standard looks like in the hepatocyte extract and decided to quantify a peak that has inexplicably drifted from a 22-min retention time to a 19-min retention time.
Previous work on FK866-resistant NMN incorporation showed quantitative dependence on nicotinamide riboside kinase 1, quantitative extracellular conversion of NMN to nicotinamide riboside and intracellular detection of nicotinamide riboside before formation of intracellular NMN1. Moreover, it has been demonstrated that modification of the phosphate in extracellularly applied NMN is required to activate the widely expressed sterile alpha and TIR motif-containing protein 1 (SARM1) to induce an NMN-dependent cell death pathway because NMN itself is not transported7.

 

 

Interesting how technical you suddenly became.

 

That sounds nothing like the language you or any laymen normally use.  Did Dr Brenner or someone at Chromadex feed you that quote, or did you copy from somwhere?


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#64 elc202

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Posted 23 May 2021 - 06:39 AM

If you are going to take NR two times per week, is it better to take it two days in a row, or separate days?

I take sulforaphane everyday.


Edited by elc202, 23 May 2021 - 06:40 AM.

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#65 LawrenceW

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Posted 27 May 2021 - 03:04 PM

If you are going to take NR two times per week, is it better to take it two days in a row, or separate days?

I take sulforaphane everyday.

 

 

Back in 2016 I took 1,000 mg of NR daily for 3 months and had zero beneficial changes in my blood biomarkers. In my opinion, it doesn't matter what regimen you use on NR, as you are not going to get any objective results anyhow.


Edited by LawrenceW, 27 May 2021 - 03:14 PM.

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#66 Phoebus

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Posted 28 May 2021 - 06:41 PM

Interesting how technical you suddenly became.

 

That sounds nothing like the language you or any laymen normally use.  Did Dr Brenner or someone at Chromadex feed you that quote, or did you copy from somwhere?

 

 

google shows its a copy and paste from a nature.com article that is behind a paywall 

 

https://www.google.c...chrome&ie=UTF-8


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#67 Phoebus

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Posted 28 May 2021 - 07:37 PM

If you are going to take NR two times per week, is it better to take it two days in a row, or separate days?

I take sulforaphane everyday.

 

 

are you eating broccoli or taking a sulforaphane supplement? 

 

Dr Jeff Fahey tested all the SF supplements on the market and found them to be junk except for one from Europe, name escapes me. He talked about this with Rhonda recently, interview can be found on YT

 

To get the most SF from broccoli try this 

 

https://www.reddit.c...large_doses_of/


Edited by Phoebus, 28 May 2021 - 07:37 PM.

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#68 aribadabar

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Posted 01 June 2021 - 06:28 PM

If you are going to take NR two times per week, is it better to take it two days in a row, or separate days?

I take sulforaphane everyday.

 

If you plan to take NR two times per week, save your money and don't bother. You WILL NOT achieve anything significant with such regimen.


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#69 elc202

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Posted 05 June 2021 - 07:25 AM

are you eating broccoli or taking a sulforaphane supplement? 

 

Dr Jeff Fahey tested all the SF supplements on the market and found them to be junk except for one from Europe, name escapes me. He talked about this with Rhonda recently, interview can be found on YT

 

To get the most SF from broccoli try this 

 

https://www.reddit.c...large_doses_of/

 

I think you mean prostaphane? it is very expensive, I saw an online store selling it now.

Jed fahey recommends others too but he says they are not as good as prostaphane, since the other ones need to be converted to sulforaphane.

He is involved with a company that produces their own broccoli extract (truebroc) and sell it to supplement companies (thorne, swanson..etc) but oddly enough I don't see myrosinase in their extract, even though several times he mentioned how important myrosinase is.

Thanks for the link, the post is great. The subreddit seems good too, i will be following it.


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#70 elc202

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Posted 05 June 2021 - 07:30 AM

If you plan to take NR two times per week, save your money and don't bother. You WILL NOT achieve anything significant with such regimen.

 

What is the minimum times per week do I need? the dosage I use is 200 or 300mg.
 

Thanks,


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#71 aribadabar

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Posted 05 June 2021 - 03:42 PM

What is the minimum times per week do I need? the dosage I use is 200 or 300mg.
 

Thanks,

 

300mg per day , every day, at minimum.


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#72 frederickson

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Posted 06 June 2021 - 01:46 PM

I think you mean prostaphane? it is very expensive, I saw an online store selling it now.

Jed fahey recommends others too but he says they are not as good as prostaphane, since the other ones need to be converted to sulforaphane.

He is involved with a company that produces their own broccoli extract (truebroc) and sell it to supplement companies (thorne, swanson..etc) but oddly enough I don't see myrosinase in their extract, even though several times he mentioned how important myrosinase is.

Thanks for the link, the post is great. The subreddit seems good too, i will be following it.

 

Great discussion.

 

Avmacol was the first product to market to contain myrosinase, which is necessary to convert glucoraphanin to sulforaphane.

 

While Prostaphane is a good product, it is very heat sensitive and hard to find in many places in the world. 

 

Avmacol has the most clinical trials published/underway of any of the commercially-available supplements (including Prostaphane) and I know that Fahey and his team have studied the product.

 

As a result, I think Avmacol is probably the best choice.


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Also tagged with one or more of these keywords: nad+, nmn, cycling, supplements, sulforaphane, sublingual, bioavailability, tolerance, downregulation

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