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methylene blue & nitric oxide

nitric oxide

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#1 farshad

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Posted 08 October 2018 - 12:50 AM


Hello, I just wanna make sure on some things that I have been reading.

 

https://en.wikipedia...#Classification

 

''There are three known isoforms in mammals, two are constitutive (cNOS) and the third is inducible (iNOS).''

 

Im assuming these three: iNOS - eNOS - nNOS

 

So what are: mtNOS & bNOS? 

 

and my second question is, is it true methylene blue decreases all types of NO enzymes (those in red)?.

 

And my third question is: since methylene blue inhibits nitric oxide and I read on wikipedia NO (nitric oxide) is involved in Smooth muscles movement. Could MB (methylene blue) possibly help your accuracy or am I reading it wrong?

 
 
''Nitric oxide (NO) contributes to vessel homeostasis by inhibiting vascular smooth muscle contraction''
 
Extra question: Since the Half-Life of MB is 5.25 h, how many times would I need to dose to keep an constant effect?

Edited by farshad, 08 October 2018 - 12:54 AM.


#2 farshad

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Posted 08 October 2018 - 05:38 AM

iNOS - inducible Nitric Oxide Synthase [this is the one we mostly want to keep down, is induced by (LPS) endotoxin and immune response and can do lots of damage and release pro-inflam chemicals]

 

eNOS - endothelial Nitric Oxide Synthase [essential for a healthy cardiovascular system; this increases vascular dilation and permeability; we want to modulate this one]
 
nNOS - neuronal Nitric Oxide Synthese [this one is found in the brain and in body's nervous tissue system, too much of this releases calcium and does damage]
 
Inducible NOS iNOS = NOS2
Endothelial NOS eNOS = NOS3 or cNOS
Neuronal NOS nNOS = NOS1
 
mitochondrial NOS (mtNOS) & bacterial nitric oxide synthase (bNOS)
 
mtNOS: Carnosine was shown to protect the mitochondria of cultured brain cells (astroglia) against nitric oxide-induced damage. (not really inhibiting tho or possible/should?)
 
bNOS: ? (missing)
 
BTW if anyone know source for MB inhibiting iNOS, eNOS and nNOS please link.
 
I also heard Aspirin inhibits these need source tho.

Edited by farshad, 08 October 2018 - 06:02 AM.


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#3 John250

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Posted 08 October 2018 - 04:34 PM

You wouldn’t want NO inhibited I would assume for blood flow.

#4 farshad

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Posted 08 October 2018 - 04:41 PM

You wouldn’t want NO inhibited I would assume for blood flow.

 

I have excess of it which is worse. Having lower NO is always better for your health. Check out what Ray Peat has to say about it.



#5 John250

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Posted 08 October 2018 - 07:10 PM

I have excess of it which is worse. Having lower NO is always better for your health. Check out what Ray Peat has to say about it.


Very interesting. The Berkeley test strips show my NO is very depleted which can’t be healthy so I assume a balance would be best. Seems the ultimate goal would be to have normal NO levels while still keeping oxidative stress low.
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#6 farshad

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Posted 09 October 2018 - 04:37 AM

MB (methylene blue) is believed to promote neuronal cell health by supporting mitochondrial function - mitochondrial NOS (mtNOS).

 

So I think there are just four NO enzymes with diff functions in the body:

bNOS

iNOS

eNOS

nNOS

 

from what I have read MB inhibits those in red but I haven't read anything about what inhibits bNOS.

And Im wondering what dosage MB inhibits NO lvls at I wanna keep the dose as low as possible to avoid interactions with other drugs since it is an MAOI-a inhibitor also and im currently on a TCA (clomipramine), Im worried it might cause serotonin syndrome.


Edited by farshad, 09 October 2018 - 05:05 AM.






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