• Log in with Facebook Log in with Twitter Log In with Google      Sign In    
  • Create Account
  LongeCity
              Advocacy & Research for Unlimited Lifespans

Photo
- - - - -

4-Hydroxy-TEMPO (Tempol)

tempol 4-hydroxy-tempo

  • Please log in to reply
3 replies to this topic
⌛⇒ write a quiz!

#1 John250

  • Member
  • 1,286 posts
  • 89
  • Location:Temecula
  • NO

Posted 09 October 2018 - 09:34 PM


Not very many discussions about this. Any experiences? Thanks

#2 Iuvenale

  • Member
  • 46 posts
  • 15
  • Location:Boulder
  • NO

Posted 27 October 2018 - 01:08 PM

I just bought some. I haven’t used it yet and I’m spooked by this article on genotoxicity.

Comparative Genotoxicity of TEMPO and 3 of Its Derivatives in Mouse Lymphoma Cells.

TEMPO (2, 2, 6, 6-tetramethylphiperidine-1-oxyl) and its derivatives are stable free radical nitroxides widely used in the field of chemistry, biology, and pharmacology. TEMPO was previously found to be mutagenic and to induce micronuclei in mammalian cells. In this study, we investigated and quantified the genotoxicity of 4 structurally similar nitroxides, TEMPO and 3 of its derivatives (4-hydroxy-TEMPO, 4-oxo-TEMPO, and 4-methoxy-TEMPO), using the mouse lymphoma assay (MLA) and Comet assay in L5178Y Tk+/- cells. The results showed that all tested nitroxides were cytotoxic and mutagenic in the MLA, both in the presence and absence of S9, with metabolic activation significantly enhancing the cytotoxicity and/or mutagenicity. In addition, the 4 nitroxides caused DNA-strand breakage. The mutagenicity and DNA damaging dose-responses of the test articles were compared using the PROAST benchmark dose software package. The potency ranking of the 4 nitroxides for mutagenicity was different from the ranking of the DNA damaging effects. The mode of action analysis by a multi-endpoint DNA damage pathway assay classified all 4 nitroxides as clastogens. In addition, the majority of the induced Tk mutants showed loss of heterozygosity at the Tk and D11Mit42 loci (ie, chromosome damage <31 Mbp). These results suggest that TEMPO and its 3 derivatives are cytotoxic and mutagenic in mouse lymphoma cells through a mechanism that involves strand breakage and large alterations to DNA. The potency rankings indicate that the different TEMPO derivatives vary in their mutagenic and DNA damaging potential.

sponsored ad

  • Advert
Click HERE to rent this advertising spot for BRAIN HEALTH to support LongeCity (this will replace the google ad above).

#3 Iuvenale

  • Member
  • 46 posts
  • 15
  • Location:Boulder
  • NO

Posted 27 October 2018 - 01:12 PM

Nature is always a chaotic mess when it comes to human values and desires. A chemical is protective, protective, protective, and then —oops— it turns out to damage DNA or have some other major destructive side effect.
  • Agree x 1

sponsored ad

  • Advert
Click HERE to rent this advertising spot for BRAIN HEALTH to support LongeCity (this will replace the google ad above).

#4 John250

  • Topic Starter
  • Member
  • 1,286 posts
  • 89
  • Location:Temecula
  • NO

Posted 27 October 2018 - 04:19 PM

Well,.... looks like my Tempol powder will remain in the freezer :(




0 user(s) are reading this topic

0 members, 0 guests, 0 anonymous users