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Running our own longevity studies?

mice laboratory study

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#31 AceNZ

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Posted 19 February 2019 - 10:52 PM

As mentioned earlier, I've been thinking about the food prep side of things.

 

Four aspects come to mind:

  1. Basic ingredients: food and nutrients
  2. Moisture content
  3. Compatibility with the pelletizer machine
  4. How palatable the result is for the mice

Coming up with a mix that forms good pellets is one thing. However, an equally important issue is whether the mice will be willing to eat the result -- and to confirm that, one would need actual mice (which I don't have).

 

I think you could approach it something like this:

  • Start with a basic mouse food like the one from Oxbow (which is already in pelletized form): https://amzn.to/2GWNbDA
  • Make sure the mice are happy with the out-of-the-bag food.
  • Put the food in a good blender and mix with just enough water to develop a paste that's compatible with the pelletizer. Be careful not to overheat the mix in the blender.
  • Run a small batch through the pelletizer, let it dry/cure (overnight?) and make sure the mice are still willing to eat the result.
  • Measure and add supplements to the mix in the blender (proportional by weight), again being careful not to overheat. Adjust moisture content if needed.
  • Repeat the pelletizer/dry/cure process.
  • Test again with the mice.
  • Store the blenderized and pelletized food in the refrig.

It may take a few iterations to perfect, but shouldn't be too bad. Should get the process nailed down with a few test mice, before the full numbers arrive.

 

Control mice should have everything the same, including blenderizing and pelletizing -- just not the added supplements.



#32 theone

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Posted 21 February 2019 - 09:20 PM

Thank you for your input, its greatly appreciated.


I will provide a full update tomorrow evening.
.

 

 



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#33 theone

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Posted 23 February 2019 - 06:48 AM

Hi Everyone, and welcome to the end of Week 3.


I am happy to report that we have installed two inline duct boosters. We will be performing the initial positive air pressure tests early next week.

We are now also protected against outages with standby generator power.

I will provide some pictures of the installation shortly.


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#34 theone

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Posted 24 March 2019 - 04:19 PM

Welcome to the beginning of week 8.

 

Here's a quick update on our recent progress.

 

1. Been experimenting with the pelletizing process. I'm starting to get the hang of how this works.

 

2. Inline HEPA filters have now arrived. Please see attached pic.

 

HEPA-filter.jpg

 

3. Positive air pressure tests successful. Please see attached pic.

 

Temp.jpg

 

top-left.jpg

 

top-right.jpg

 

We plan to make the final decision on the  IVC cage system within the next 2 weeks. 


Edited by theone, 24 March 2019 - 04:28 PM.

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#35 AceNZ

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Posted 24 March 2019 - 07:39 PM

Awesome. Looks like great progress!

 



#36 Phoebus

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Posted 24 March 2019 - 09:08 PM

Welcome to the beginning of week 8.

 

Here's a quick update on our recent progress.

 

 

 

Hey this looks great! 

 

have you decided if you are going to test NR and/or NMN yet? 



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#37 theone

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Posted 25 March 2019 - 08:18 PM

I would prefer to use both NR and NMN  (with a larger sample size).    We can also go with multiple experimental groups at one time.

Please let me know your thoughts.



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#38 theone

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Posted 09 July 2019 - 06:28 PM

My apologies for the delayed update.  Took a leave of absence due  getting married. :wub:   So many things to catch up on. Hope to get this project back on track within the next few weeks

 

 

 

 







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