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TRIIM Protocol without HGH and with some additional modifications

epigenetics triim telomeres

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#1 GreenPower

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Posted 22 September 2019 - 01:35 PM


After the most interesting results of the recent TRIIM study I've been reading up on the subject.

 

First I was kind of overwhelmed with the requirements, time and money needed for trying to replicate and/or incorporate the protocol in my own regimen. Then I started to feel it's actually a challenge :).

 

I've therefore started to draft a regimen based on the TRIIM Protocol which I think I might be able to start in a month or so. Currently I'm collecting the necessary substances and tests needed. I've excluded HGH (Omnitrope) due to its lack of easy availability and will instead include some other substances which I think might be beneficial. From a testing point of view there will be no MRI's but some other tests with an emphasis on telomeres.

 

Because the the modified protocol is kind of ambitious I wonder if you see any obvious mistakes or easy improvements to it.

 

Below I've added is my first draft.

 

TRIIM Protocol without HGH and with some additional modifications

HGH has been left out because it seem to be a bit hard to obtain.

Pregnenolone has been included on a hunch.

Astragalus has been included since I'm currently using it (baseline/what I'm using today: once per day in the morning).

Gingko Biloba has been included since I'm currently using it (baseline: 1-3 times per week, usually around lunch).

 

Besides the substances below I consider the following to be included:

- Training sessions 2-3 times per week (baseline: currently 1 time per week)

- Regular saunas 4-5 times per week in order to decrease heavy metals (baseline: 1 time per half year)

- Transcendental Meditation at least 4-5 times per week around lunch (baseline: non-existent)

- Lower environmental stress (baseline: quite high)

- Low Carb High Fat diet (baseline: I'm currently in ketosis only one week every month or so)

 

1st week

Baseline tests

  • Baseline blood tests: "Healthcheck XL+" with added Zinc, Cortisol, DHEAS, Thyroid tests. All tests during the morning.
  • Baseline blood test: myDNAge Epigenetic Aging Clock test, around lunch
  • Baseline blood test: RepeatDX 6-Panel assay Telomere length test, around lunch.
  • Baseline blood test: LifeLength TAT, around lunch
  • Baseline Saliva tests: Cortisol (morning), DHEA (morning)
  • Baseline Heavy Metal Urine Test: 17 substances (Aluminum, Antimony, Arsenic, Lead, Cadmium, Chromium, Cobalt, Copper, Nickel, Palladium, Mercury, Iron, Platinum, Silver, Thallium, Zinc and Pewter), morning.
  • uBiome Five Site bacterial tests: Gut, Mouth, Nose, Genitals, Skin)

 

Morning

1 x 25 mg DHEA

1 x 30 mg Pregnenolone

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

Lunch

1x 105 mg Gingko Biloba

 

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

 

2nd week

Adjustment tests

Blood

  • CRP, Hb, LPK, TPK, MCH, MCHC, MCV, EPK, EVF
  • DHEAS
  • HbA1c (glycated hemoglobin)
  • Glucosis
  • C-Peptide

Saliva

  • Cortisol (morning), DHEA (morning)

 

Morning

1 x 25 mg DHEA

1 x 30 mg Pregnenolone

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

Lunch

1x 105 mg Gingko Biloba

 

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

 

3rd week

Morning

2 x 25 mg DHEA (dose change to 50 mg if adjustment test results support this)

1 x 100 mg Pregnenolone (dose change to 100 mg if adjustment test results support this)

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

Lunch

1 x 500 mg Glucophage (Metformin)

1x 105 mg Gingko Biloba

 

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

 

4th week

Adjustment tests

Blood

  • CRP, Hb, LPK, TPK, MCH, MCHC, MCV, EPK, EVF
  • DHEAS
  • HbA1c (Glycated hemoglobin)
  • Glucosis
  • C-Peptide

Saliva

  • Cortisol (morning), DHEA (morning)

 

Morning

2 x 25 mg DHEA (dose change if adjustment test results support this)

1 x 100 mg Pregnenolone (dose change if adjustment test results support this)

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

Lunch

1 x 500 mg Glucophage (Metformin)

1x 105 mg Gingko Biloba

 

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

 

5th to 52nd week

Morning

1 x 25 mg DHEA

1 x 30 mg Pregnenolone

2 x 25 mg Zinc, organic

1 x 2500 IE Vitamin D3

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

Lunch

1 x 500 mg Glucophage (Metformin)

1 x 105 mg Gingko Biloba

 

Evening

1 x 250 mg Root Powder/225 mg Root Powdered Extract Astragalus

 

 

Week 53

Tests for results

  • Blood tests: Healthcheck XL+ with added Zinc, Cortisol, DHEAS, Thyroid tests. All tests during the morning.
  • Blood test: myDNAge Epigenetic Aging Clock test, around lunch.
  • Blood tests: RepeatDX 6-Panel assay Telomere length test, around lunch.
  • Blood test: LifeLength TAT, around lunch.
  • Saliva tests: Cortisol (morning), DHEA (morning)
  • Heavy Metal Urine Test: 17 substances  (Aluminum, Antimony, Arsenic, Lead, Cadmium, Chromium, Cobalt, Copper, Nickel, Palladium, Mercury, Iron, Platinum, Silver, Thallium, Zinc and Pewter), morning.
  • uBiome Five Site bacterial tests: Gut, Mouth, Nose, Genitals, Skin

 


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#2 Kentavr

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Posted 23 September 2019 - 09:44 AM

HGH is available. It is used by bodybuilders. There are even instructions on YouTube how to inject it with a syringe.

However, if you do not want to use growth hormone, you can try using Ubiquinol in an amount of 200-300 mg. It will stimulate the production of ATP, which approximately can be taken as an acceleration of metabolism.
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#3 Kentavr

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Posted 23 September 2019 - 09:55 AM

There is one feature when taking ubiquinol: its effect is not immediately felt.

It is necessary to drink at least 1 month to feel the powerful effect. And you have to take it every day.

Only the effect can be a little smaller. Growth hormone works after injecting for 2 hours, then its effect drops sharply. I think that the feature of the TRIIM protocol is this: when IGF-1 was reduced using metformin, we inject growth hormone at that moment. In this case, forced cell division occurs with reduced IGF-1. A decrease in IGF-1 gives increased expression of FOXO1, which stimulates the symmetrical division of its own stem cells.

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#4 Kentavr

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Posted 23 September 2019 - 10:00 AM

I’m afraid without growth hormone it won’t work. But you can try to stimulate the division with the help of Ubiquinol. But it will be a constant weak "pressure", and not a strong "pressure" for 2 hours.
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#5 Kentavr

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Posted 23 September 2019 - 02:23 PM

Yes, and again: it seems that the activation of telomerase accelerates epigenetic drift.

You can get the opposite effect - the acceleration of epigenetic hours due to the activation of telomerase.

#6 Kentavr

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Posted 23 September 2019 - 02:35 PM

What drugs would I include in addition?

L-threonine is likely to facilitate the division of specific stem cells.

Before proceeding to your protocol, I advise you to fully read the topic:

https://www.longecit...newal-with-c60/

There you will find information on L-threonine.

#7 Kentavr

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Posted 23 September 2019 - 03:02 PM

In addition, I would increase the dosage of metformin from 500 mg to 650 mg, or 750 mg.

Probably, in this case the effect will be stronger (because the decrease in IGF-1 will be more significant).

#8 Fafner55

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Posted 25 September 2019 - 03:12 PM

10 to 15 mg / day MK-677 at bedtime should sufficiently increase hGH. MK-677 is readily available on the internet without prescription.

  1.  “Effects of an Oral Ghrelin Mimetic on Body Composition and Clinical Outcomes in Healthy Older Adults: A Randomized Trial” (2008)  http://annals.org/article.aspx?articleid=743451
  2. “MK-677, an Orally Active Growth Hormone Secretagogue, Reverses Diet-Induced Catabolism” (1998) https://academic.oup.com/jcem/article/83/2/320/2865101
  3. “Two-Month Treatment of Obese Subjects with the Oral Growth Hormone (GH) Secretagogue MK-677 Increases GH Secretion, Fat-Free Mass, and Energy Expenditure” (1998) http://press.endocrine.org/doi/full/10.1210/jcem.83.2.4539
  4. “Adult GH deficiency throughout lifetime” (2009) http://eje-online.org/content/161/suppl_1/S97.full
  5. “Evening versus morning injections of growth hormone (GH) in GH-deficient patients: effects on 24-hour patterns of circulating hormones and metabolites” (1990) https://www.ncbi.nlm.nih.gov/pubmed/2294131

 


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#9 GreenPower

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Posted 25 September 2019 - 08:38 PM

I think MK-677 might be the most interesting option.

- It seem to mimic HGH.

- It can be taken orally (no needles).

- It seem to have been widely used without serious side-effects reported.

- High glucose levels and insulin resistance have been reported, but this should be handled by adhering to a Low Carb High Fat diet in combination with Metformin.

- Does not affect cortisol levels.

 

It understand it to be sort of legal, although not many serious vendors in Europe seem to sell it. I suppose this is because it's still experimental and also considered forbidden for usage in sports.

 

If I get hold of it, I will try to go with 15mg/day. It seem to be well below the maximum limit of 25mg/day which some sites seem to recommend.


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#10 lukas_93

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Posted 03 October 2019 - 07:57 PM

MK-677 sounds like a great idea! If you are located in Europe, importing Omnitrope from Russia might also be an approach. Rupharma sells HGH as Getropin for example. Getting it through customs might be a problem, though. 


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#11 Brundel

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Posted 29 October 2019 - 01:30 AM

For increased HGH levels Impamorelin and cjc works exceptionally well.

100mcg of each 5 days a week is enough.

GH secretagogues.

Relatively inexpensive online at bodybuilding research chem sites.


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#12 Fafner55

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Posted 29 October 2019 - 10:51 AM

 

10 to 15 mg / day MK-677 at bedtime should sufficiently increase hGH. MK-677 is readily available on the internet without prescription.

 

 

10 mg MK-677 is not enough. I took that dose before bed for more than 2 weeks then had an early morning blood test. The results showed a hGH level of nearly zero. Since then I upped the dose to 20 mg. I am 64 years old.


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#13 Fafner55

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Posted 29 October 2019 - 10:59 AM

Here are additional references for MK-677 and other growth hormone secretagogues including Impamorelin.

 

“Effect of Alendronate and MK-677 (a Growth Hormone Secretagogue), Individually and in Combination, on Markers of Bone Turnover and Bone Mineral Density in Postmenopausal Osteoporotic Women” (2001) https://academic.oup.com/jcem/article/86/3/1116/2847590/

 

“The Safety and Efficacy of Growth Hormone Secretagogues” (2017) https://www.smr.jsexmed.org/article/S2050-0521(17)30032-X/pdf


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#14 Brundel

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Posted 29 October 2019 - 08:29 PM

10 mg MK-677 is not enough. I took that dose before bed for more than 2 weeks then had an early morning blood test. The results showed a hGH level of nearly zero. Since then I upped the dose to 20 mg. I am 64 years old.

 

Have you retested?

Where did you obtain your Mk-677? Lots of the research stuff is fake.

One of the benefits of Ipamorelin and CJC is the right dr can script it for you. Its sort of expensive but nothing compared to serostim or the like.

I think I pay 475$ for 75 days worth. Aside from its use in the TRIIM protocol I can show from labs that its very effective and I can say anecdotally that its effects are noticeable.

Muscle and strength does rise and bodyfat drops over the course of the first round of treatment.

 


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#15 Fafner55

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Posted 30 October 2019 - 12:51 PM

Have you retested?

Where did you obtain your Mk-677? Lots of the research stuff is fake.

One of the benefits of Ipamorelin and CJC is the right dr can script it for you. Its sort of expensive but nothing compared to serostim or the like.

I think I pay 475$ for 75 days worth. Aside from its use in the TRIIM protocol I can show from labs that its very effective and I can say anecdotally that its effects are noticeable.

Muscle and strength does rise and bodyfat drops over the course of the first round of treatment.

 

I have not retested since upping the dose to 20 mg.

 

Directly testing hGH is not particularly reliable in the sense that it can vary widely within a day. In hindsight, I should have tested for IGF-1 instead as most researchers did in the papers I cited. Next time I'll test for both hGH and IGF-1.

 

I don't doubt that MK-677 is effective. Even at 10 mg when I started, I could sense firmer muscle tone and some aches in the joints of my hands within the first week. The mimor aches subsided within a week as my body adjusted to the changes.



#16 Smith

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Posted 12 November 2019 - 08:45 PM

I also agree that without some form of HGH the protocol will not likely work.  The whole point of HGH was rejuvenation of the Thymus, w/o which, the major benefit is lost.

 

MK-677 should work as a substitute, as it is an analog of HGH with even fewer side effects and is known to boost IGF-1.  The question remains the dosing.   5mg might be enough to boost IGF-1 levels, but without testing, its hard to know.  Hope someone can post their test results, or point out where one can obtain inexpensive IGF-1 blood tests.

 

EDIT: Found that you can get IGF-1 blood test for $69 and IGF-1 + HGH test for $97 at UltaLabTests.  You can also find some coupon codes to drop the price even further.


Edited by Smith, 12 November 2019 - 08:55 PM.


#17 William Sterog

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Posted 18 November 2019 - 09:32 AM

Well. Reliable source af MK-667 in Europe? It is worth it to try this protocol at 27 if you already feel like shit?

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#18 aribadabar

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Posted 18 November 2019 - 06:52 PM

It is worth it to try this protocol at 27 if you already feel like shit?

 

Your thymus is involuting since puberty so chances are it can benefit from restoration even at 27. You just have less work to do than older people  :cool:







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