2 replies to this topic

[MikeDC]

Scientists have spent decades studying aging caused by oxidative stress and damage accumulation. 4-HNE is a toxic waste from oxidative damage, especially related Linoleic acid oxidation. But no one can conclusively confirm that 4-HNE is the root cause of aging. I am sure you all have heard that OSKM and OSK reprogramming cured blind mice, monkeys. It is in clinical trial. Ryan Lu studied OSK therapy further and found that what cured the blind sight is due to GSTA4 over expression. GSTA4 is a well known gene that clears 4-HNE with glutathione as cofactor. GSTA4 is sufficient and required to remove 4-HNE and restore RPE function. So this paper has shown that 4-HNE is the most important factor in aging.

https://www.biorxiv.....08.30.673239v1


So it has been proven that we can rejuvenate cells by removing 4-HNE. There are perfect supplements just for this purpose. Sulforaphane can upregulate NRF2 which upregulate GSTA4. There are studies that show Sulforaphane can increase GSTA4 by a few hundred percent. 100mg/kg glycine and NAC has been proven to increase glutathione by a significant amount. So combining these 3 together could replicate OSK reprogramming’s rejuvenation effect. If you want the references you can ask AI on Google. I think this is the most significant finding in our hunt for why we age and how to slow it down.

Outside of this I want to tell people again, liposomal CaAKG has tremendous aging reversal effects. I feel like I am at least 20 years younger after taking it for 3 years. If you combine these two schemes, age reversal is reality. I am going to add Sulforaphane and glycine plus NAC to my daily supplement soon. Fully expecting to get even younger.

[MikeDC]

The general safe upper limit for daily oral N-Acetylcysteine (NAC) supplementation is typically considered to be 3,000 mg per day, though most standard and effective doses range from 600 mg to 1,200 mg per day.Standard and High DosagesStandard Daily Dose: 600 mg to 1,200 mg split into one or two doses is commonly used for general antioxidant and respiratory support.Supervised Maximum Dose: Clinical studies and therapeutic protocols safely utilize up to 2,400 mg to 3,000 mg per day for specific respiratory or psychiatric conditions under medical supervision.Toxicity Threshold: Doses exceeding 3,000 mg daily significantly increase the likelihood of side effects without added clinical benefit, and total cysteine intake should stay well below toxic limits.

[MikeDC]

There is no clinical evidence that standard doses of N-acetylcysteine (NAC) cause damage to the blood-brain barrier (BBB) in humans.Current Evidence on NAC and the Blood-Brain Barrier. Low Human Data: No human clinical trials or standard medical data link standard oral or intravenous NAC administration to structural or functional breakdown of the blood-brain barrier.Limited Permeability: NAC has relatively poor and limited permeability across the human blood-brain barrier due to its low bioavailability (often around 6% to 10%) and negative charge at physiological pH.Preclinical Animal Findings: Isolated animal studies (such as in specific stroke-prone or genetically hypertensive rodent models) have raised theoretical questions about high doses or specific pathological states altering barrier dynamics, but these findings have not translated to evidence of human BBB damage during standard use.Protective Effects: In several preclinical models of brain injury or inflammation, NAC actually helps protect or restore vascular and blood-brain barrier integrity by reducing oxidative and nitrosative stress.

Edited by MikeDC, Today, 04:15 PM.