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The Official Milk Thistle / Silymarin Thread


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#91 Jiminy Glick

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Posted 03 February 2018 - 01:15 PM

What is a good silymarin and silybin ratio?



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#92 Olive_skin

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Posted 08 March 2023 - 06:10 PM

I have come across a patent application from 2012 [1]. Its inventor claims, that his composition of extracts can increase the telomere length in humans. The main ingredient was milk thistle (50%). He choose the substances with following lab test:

 

 

An outside laboratory, Sierra Sciences of Reno, Nevada, was engaged to screen samples to determine induction of hTERT mRNA in normal human BJ fibroblast cells.

Composition of the samples was not disclosed to the laboratory, and test results were recorded by sample number.
[0017] Each sample was diluted in DMSO to create a 50.00 mg/mL stock solution. After DMSO addition, each stock solution was sonicated for 2 minutes and centrifuged at 291 x g for 1 minute. An aliquot of the supernatant from each 50.00 mg/mL stock solution was taken and diluted in an 8-point dilution series (n=8, d=3, CH=50.00 mg/mL, CL=0.02 mg/mL). 1.00 of each dilution point was used to treat 10,000 (or 2,000 for CV90) BJ cells in triplicate with 150 of cell media in each well.
[0018] Final treatment concentrations were: 333.33 μg/mL; 111.11 μg/mL; 37.04 μg/mL; 12.35 μg/mL; 4.12 μg/mL; 1.37 μg/mL; 0.46 μg/mL; and 0.15 μg/mL. A 96-well format was implemented using 10,000 cells per well for hTERT RT-PCR and CV24 (24 hour treatment). 2,000 cells per well were used for CV90 (90 hour treatment).
[0019] When RT-PCR produced two or more replicates at a particular concentration wherein the number of induced hTERT transcripts was greater than zero, the sample was considered an hTERT Positive Sample (Hit)....
 [Silymarin at 80% concentration] Three positive replicates at 111.11 μg/mL, with an average hTN of 47.34 (4-fold less than C0057684)....

The test was proceeded for all the other samples. C0057684 is a drug-like compound, that activates telomerase, and was used as a positive control.

Then he mashed all "hit" ingredients together into his composition, which ended up to be: horny goat weed (Epimedium sagittatum), Grape Seed (Vitis vinifera), Turmeric (Curcuma longa), Bacopa (Bacopa monnieri), Pomegranate (Punica granatum), DL-alpha lipoic acid, Asian ginseng, (Panax ginseng), Green Tea, White Tea, Black Tea (Camellia sinensis), Acacia (Acacia nilotica), Plantain (Plantago major), L-glutathione, Velvet Bean (Mucuna pruriens), Hawthorn (root) (Crataegus pinnatifida), Quercetin, Boswellia, (Boswellia serrata), Maca (Lepidium meyenii), Hawthorn (fruit) (Crataegus pinnatifida), Resveratrol, Harada (Terminalia chebula), Shilajit, Chia (Salvia hispanica), N-Curcusorb (trade name for version of Turmeric), Polygonum Cuspidatum (trans resveratrol), pterostibene, (a synthetic form of resveratrol developed by ChronaDex Company), Tumipure (trade name for Turmeric ingredient by Naturex Company). A 52 year old male was then used as a "working example". After taking 6 capsules of the final product daily for four months his average telomere length from his nucleated white blood cells went from 8.3 kp to 8.44 kp. 

[1] ANDERSON, John W. (2012-08-09). Compound and method for increasing telomere length. https://patents.goog...2012106692A1/en


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