8 replies to this topic

[pleb]

i came across this in a reply by a poster on Vince Giuliano's blog and thought it might prove interesting especially to those who have much more knowledge than me, (thats most of the guys on here) :>)


fullerene C60 results in increased expression of SIRT6 mRNA –http://rgd.mcw.edu/r...3128&id=1305216
(see the supplemental table in the mmc1.xls attachment for PMID 19167457)

[AdamI]

and how much was the increase, if they could measure it...?

[pleb]

i have no idea, but it seems to confirm that C60 is more than just an antioxidant,

I'm sure some of the more knowledgeable posters may have more of an idea,,

[niner]

Here's the original research, and it doesn't even mention sirt6. Perhaps more importantly, this is not molecular c60, but rather a case of rats breathing nanoparticle dispersions. This is probably about the body's response to particulate junk in the lungs. In the paper, they find that c60 particles don't change expression much, while nickel oxide particles change it a lot. If Sirt6 shows up with c60 but not nickel oxide, then it would be very interesting, because Sirt6 knockouts develop progeria and die early, while overexpression results in ~15% life extension in male mice. (see wiki, and links therein.)

Toxicology. 2009 Apr 5;258(1):47-55. doi: 10.1016/j.tox.2009.01.005. Epub 2009 Jan 9.
Gene expression profiles in rat lung after inhalation exposure to C60 fullerene particles.
Fujita K, Morimoto Y, Ogami A, Myojyo T, Tanaka I, Shimada M, Wang WN, Endoh S, Uchida K, Nakazato T, Yamamoto K, Fukui H, Horie M, Yoshida Y, Iwahashi H, Nakanishi J.

Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Onogawa 16-1, Tsukuba, Ibaraki 305-8569, Japan. ka-fujita@aist.go.jp

Concern over the influence of nanoparticles on human health has risen due to advances in the development of nanotechnology. We are interested in the influence of nanoparticles on the pulmonary system at a molecular level. In this study, gene expression profiling of the rat lung after whole-body inhalation exposure to C(60) fullerene (0.12mg/m(3); 4.1x10(4) particles/cm(3), 96nm diameter) and ultrafine nickel oxide (Uf-NiO) particles (0.2mg/m(3); 9.2x10(4) particles/cm(3), 59nm diameter) as a positive control were employed to gain insights into these molecular events. In response to C(60) fullerene exposure for 6h a day, for 4 weeks (5 days a week), C(60) fullerene particles were located in alveolar epithelial cells at 3 days post-exposure and engulfed by macrophages at both 3 days and 1 month post-exposures. Gene expression profiles revealed that few genes involved in the inflammatory response, oxidative stress, apoptosis, and metalloendopeptidase activity were up-regulated at both 3 days and 1 month post-exposure. Only some genes associated with the immune system process, including major histocompatibility complex (MHC)-mediated immunity were up-regulated. These results were significantly different from those of Uf-NiO particles which induced high expression of genes associated with chemokines, oxidative stress, and matrix metalloproteinase 12 (Mmp12), suggesting that Uf-NiO particles lead to acute inflammation for the inhalation exposure period, and the damaged tissues were repaired in the post-exposure period. We suggest that C(60) fullerene might not have a severe pulmonary toxicity under the inhalation exposure condition.

PMID: 19167457

[pleb]

thanks niner , i simply copied the entire paragraph from the guy who had responded to VG's article on the other site including the sentence before and after the link,

[Logic]

Hmmm... I wonder if the upregulation of SIRT6 by C60 might be mentioned later in the study and not in the abstract as this link to an annotation looks legitimate:
http://rgd.mcw.edu/r...3128&id=1305216

Thx for the very interesting info Pleb. This is certainly worth looking into further!

The 3 blog posts are also really worth reading as they outline a supp stack that is both anti-cancer and life extending.

[niner]

If you want to look at the supplemental data, here it is, all 1705 entries. There is no magnitude information, only "increases" or "decreases", so it's not very useful. Hardly useful at all, actually. In the paper, they really downplayed the gene expression alterations from c60, which leads me to think that most of the magnitudes are small. Expression change data without magnitudes is pretty much just noise, imho.

[Logic]

Thx Niner

Its a pity this info is next to useless.

[pleb]

i agree, thanks niner can you delete the thread,

Edited by pleb, 07 June 2013 - 09:48 PM.